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Citations for PerkinElmer :
301 - 350 of 1506 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase measurements were taken every other day beginning from day 2 (the day after the injection) – mice were anaesthetised (isoflurane inhalation) and subcutaneously injected with Xenolight D-luciferin substrate (PerkinElmer). Mice were imaged after 5min using the IVIS Lumina II (Caliper Life Sciences) ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3.7 MBq/mL) and [2-14C] acetic acid (2.183 GBq/mmol, 37 MBq/mL) were purchased from PerkinElmer (Waltham, MA). For the ultra[performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was scanned and images were acquired in real-time every 2 hours from 0 to 24 h post-treatment by Opera Phenix High Content Screening System by PerkinElmer, Inc ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1-2 μg of total RNA was used to isolate poly-A RNA with NEXTFLEX Poly(A) beads 2.0 (Perkin Elmer), while libraries were prepared using the NEXTFLEX Rapid Directional RNA-seq Kit 2.0 (PerkinElmer) ...
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bioRxiv - Microbiology 2024Quote: ... plates were incubated in the dark at 37°C + 5% CO2 before their fluorescent signal was measured at 2 hours post-incubation at 485/535nm using the Victor3V microplate reader (PerkinElmer). Background signal from unstimulated cells were subtracted from zymosan stimulated samples for analysis ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with Ham’s F-10 media supplemented with 1.5 mCi/well myo[2-3H(N)] inositol (Perkin Elmer/Revvity) for 16-18 h ...
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bioRxiv - Biochemistry 2024Quote: ... Radioactivity was measured (counts per minute, CPM) using a scintillation counter (MicroBeta 2 Plate Counter) with a scintillation cocktail (OptiPhase SuperMix, PerkinElmer). Data were analyzed via nonlinear regression in GraphPad Prism v10.
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bioRxiv - Developmental Biology 2020Quote: ... Staining was visualized with DAB or TSA Plus Cyanine 5 (NEL745001KT, PerkinElmer; 1:750). Combined ISH and immunofluorescence (IF)/immunohistochemistry (IHC ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was initiated with addition of 12 pmol rrnBP1 PCR promoter fragment pre-mixed with 1 mM ATP and 4 μCi α-[32P]-CTP (3,000 Ci mmol−1; Perkin Elmer) at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ppm and 1.5 ppm (Multi-element calibration standard 3 for ICP, 10µg/ml, PerkinElmer, L2-20MKBY1). The metal homeostasis analysis is done in Perkin-Elmer Optima 700 DV ICP optical emission spectrometer version 5.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 41.3 Ci mmol−1) and radiocarbon-labelled serotonin binoxalate (14C-serotonin; 54.0 mCi mmol−1) were purchased from Perkin Elmer (Waltham, Massachusetts, USA). Serotonin creatinine sulphate ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the amount of tritiated acetate in the organic phase was measured by liquid scintillation counting for 2 min (Tri-Carb 2900TR, Perkin Elmer). Each sample was assayed in triplicate and counts were corrected for background by subtraction of counts obtained using whole blood samples devoid of cholinesterase activity and treated in a similar fashion ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Developmental Biology 2021Quote: ... Successfully mounted embryos were then imaged starting between 2 and 7 ss using a 20X objective on a Perkin-Elmer UltraVIEW Vox spinning disk confocal microscope (Perkin Elmer) while maintained at 32°C ...
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bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
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bioRxiv - Microbiology 2020Quote: Infected mice were anesthetized using isofluorane and 1 μl of decyl aldehyde was instilled into each nostril before placement in a BSL-2 chamber within the IVIS instrument (IVIS Spectrum, Perkin Elmer). Isofluorane anesthesia was sustained during IVIS imaging ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was separated in 2% glyoxal agarose gels and blotted onto a positively charged nylon membrane (GeneScreen Plus, Perkin Elmer). [32P]-labelled probes specific for each transcript were generated by linear PCR ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 6-well plates were incubated with 2 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [14C(U)]-glutamine (Perkin Elmer) for 6 hours ...
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bioRxiv - Immunology 2021Quote: ... Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Neuroscience 2022Quote: ... 1:200 in TNB buffer or NGS for 2 hours at room temperature and then incubated with HRP-Conjugated Streptavidin (PerkinElmer; USA) 1:100 in TNB buffer or NGS for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured for 90 minutes at 2-min intervals using a 340/460 excitation/emission filters on a Envision plate reader (Perkin Elmer). The IC50 values were calculated by fitting the curves using a four-parameter equation in GraphPad Prism ...
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bioRxiv - Microbiology 2022Quote: ... Cells (2 × 104 cells/well) were seeded into 96-well plates (Cell Carrier-96well Ultra microplate; PerkinElmer Inc., Waltham, MA, USA) and differentiated to macrophages with phorbol 12-myristate 13-acetate (10 ng/mL ...
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bioRxiv - Biochemistry 2022Quote: ... A competitor unlabelled DNA of the same sequence was added at 2 µM and FP was measured over time on a VictorV plate reader (Perkin Elmer). Experiments with PARP1 were carried out in the same conditions as for PARP2 with the exception that the reaction buffer which was 12 mM Hepes pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... 2 x 105 parasites were applied to each well of a clear-bottomed 96-well plate (Perkin Elmer cat. no. 6055300) seeded with HFF cells ...
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bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
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bioRxiv - Microbiology 2024Quote: ... 2×106 bacteria/mL in PBS were seeded in 384-well black clear-bottom plate (ViewPlate®-384 F TC, PerkinElmer) in the presence of 12.5 % BRC ...
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bioRxiv - Physiology 2023Quote: ... the animals were fasted overnight and a bolus of 2-deoxy-D-[1,2-3H]-glucose (1mC/ml; specific activity: 20Ci/mmol; PerkinElmer, Madrid, Spain) mixed with glucose (100µCi/kg body weight ...
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bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
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bioRxiv - Microbiology 2024Quote: ... Sample volumes were then normalized to 2 mL and determined by inductively coupled plasma mass spectrometry (ICP-MS) with a NexION 300X instrument (PerkinElmer, USA). To detect intracellular iron ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant and precipitate were separated and both fractions were counted in a gamma counter (Wizard 2 2480, Perkin Elmer, USA). Following this ...
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bioRxiv - Microbiology 2024Quote: ... were reactions (final volume of 200 μl) were performed in microtiter plates and recorded with a Victor-2 plate reader (Perkin Elmer) Every experiment was done with two biological samples and three technical replicates.
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bioRxiv - Cell Biology 2024Quote: Hep G2 and U-2 OS cells are counted and seeded into 384 well plates (PhenoPlate 384-well, Perkin Elmer, 6057328) using a Biotek or Multidrop microplate dispenser ...
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bioRxiv - Physiology 2024Quote: Metabolic flux assessment using the Seahorse XFe analyser and 2-[1,2-3H(N)]-deoxy-D-glucose (3H-2DOG; Perkin Elmer, USA) uptake assays were performed on scramble and siPKD1/2/3 transfected 3T3-L1 differentiated adipocytes five days post-differentiation in 24-well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2 and 1 mM DTT) supplemented with 0.76 μM radioactively labelled AdoMet (PerkinElmer) for 4 h at 37 °C or overnight at 25 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2 and 1 mM DTT) supplemented with 0.76 μM radioactively labelled AdoMet (PerkinElmer) for 4 h at 37 °C or overnight at 25 °C ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) overnight ...
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bioRxiv - Biophysics 2024Quote: ... A six-point standard curve from 200 ppb to 1 ppb (200, 100, 50, 10, 5, 1) was prepared from elemental standards (Perkin Elmer), plus a 0-ppb blank ...
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bioRxiv - Bioengineering 2020Quote: ... In vivo and ex vivo fluorescence imaging was carried out using a planar (2-D) IVIS Spectrum device (PerkinElmer, Waltham, MA, USA). Pymol (1.7 ...
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bioRxiv - Neuroscience 2020Quote: ... were harvested in Schneider’s medium on ice and transferred to Schneider’s medium containing 35S-methionine/cysteine (2 µCi/µl; Perkin Elmer Labs Inc., NEG709A) and 30 µM chloroquine (to inhibit autophagy) ...