Labshake search
Citations for PerkinElmer :
351 - 400 of 1032 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2022Quote: ... the membrane was transferred to a fresh falcon tube and incubated with 1ml of Western LightningPlus-ECL chemiluminescent reagent (PerkinElmer). Proteins bands were visualised with an iBright instrument (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... the membranes were washed 5 min 3 times and developed using enhanced chemiluminiscence reagent (Western Lightning Plus-ECL; Perkin Elmer). The following primary antibodies were used ...
-
bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
-
bioRxiv - Microbiology 2022Quote: Radioactivity of cell wall and membrane isolates was measured by scintillation counting using a Liquid Scintillation Analyzer Tri-Carb 4910 TR (PerkinElmer). To do this ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA samples were separated on a 7 M urea denaturing polyacrylamide gel at 4°C at 100V and transferred onto a charged nylon transfer membrane (PerkinElmer), followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
-
bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
-
bioRxiv - Developmental Biology 2023Quote: ... membrane staining was added to single wells immediately before imaging on an Opera Phenix™ High-Content Screening System (PerkinElmer).
-
bioRxiv - Neuroscience 2024Quote: ... The membranes were washed in TBST at least three times and probed with Western Lightning Plus ECL reagent (PerkinElmer, NEL105001EA) and exposed to GE Healthcare Hyperfilm ECL (28906839) ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... and membranes were incubated 1 min with in a 1:1 mixture of ECL solutions A and B (PerkinElmer, USA). Excess ECL solution was removed ...
-
bioRxiv - Neuroscience 2024Quote: ... the membranes were incubated for 90 seconds in 1 mL of enhanced chemiluminescence (ECL Pro) reagent (catalog number NEL122001EA, PerkinElmer Health Sciences Canada Inc. ...
-
bioRxiv - Cell Biology 2022Quote: Assay ready plates with pre-dispensed compounds were prepared in black 384-well imaging plates (Perkin Elmer, PhenoPlate 384-well microplates ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then lysed using Britelite plus luminescence reporter gene assay system (Perkin Elmer Catalog no. 6066769) and RLU were measured using the Biotek plate reader ...
-
bioRxiv - Cancer Biology 2021Quote: ... firefly luciferase activity was measured using the Britelite Plus Reporter Gene Assay System (Perkin Elmer, Waltham, MA) on a Synergy HT luminometer (BioTeck ...
-
bioRxiv - Cell Biology 2022Quote: ... Luminescence signals of the CellTiter-Glo assay were detected on the EnVision 2104 Multilabel plate reader (PerkinElmer). Signals of the Ferrostatin-1-treated wells were set to 100% activity ...
-
bioRxiv - Microbiology 2022Quote: ... The AlphaLISA assay development was performed in 96-well 12 area Alphaplate (reference 6052340, PerkinElmer, Waltham, MA) with a final reaction volume of 40 μL ...
-
bioRxiv - Neuroscience 2022Quote: ... Aβ oligomers were detected by 30 μl of 0.2 mg/ml 82E1 in DELFIA assay buffer (PerkinElmer) for 1 h at 25 °C with shaking at 400 RPM ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... All assay plates used were OptiPlate-384 white well microplates (product number: 6007290, PerkinElmer LAS Ltd, UK) unless otherwise stated ...
-
bioRxiv - Molecular Biology 2022Quote: ... and luciferase activity was measured with the twinlite Firefly and Renilla Luciferase Reporter Gene Assay System (PerkinElmer). Means of triplicate were calculated for each sample and Firefly/Renilla ratio of stimulated wells was normalized to that obtained in non-stimulated wells.
-
bioRxiv - Microbiology 2023Quote: ... cells were lysed using the Britelite plus reporter gene assay system (PerkinElmer, Waltham, MA, USA, Cat# 6066769), and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
-
bioRxiv - Biophysics 2023Quote: ... Infectivity was measured by using the Steady-lite plus luminescence reporter gene assay system (PerkinElmer, Waltham, MA). Luciferase activity was measured by adding 100 μL of Steady-lite plus buffer (PerkinElmer ...
-
bioRxiv - Immunology 2024Quote: ... the luciferase activity was measured using a dual-luciferase reporter assay system (EnVision™; PerkinElmer Co., Ltd.), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Luciferase activity was measured using Dual-Glo luciferase assay system by a Envision II plate reader (PerkinElmer) 48 hours after compound addition.
-
bioRxiv - Biophysics 2024Quote: Compounds dissolved in DMSO were dispensed into white ProxiPlate-384 Plus 384-well assay plates (PerkinElmer,6008280) using an acoustic liquid handler (Echo 650 ...
-
bioRxiv - Biophysics 2021Quote: ... add 10 mL scintillation solution and measured the radioactivity retained on the membranes in liquid scintillation counter Tri-carb 4910TR (Perkin Elmer) [44].
-
bioRxiv - Cell Biology 2020Quote: ... the PVDF membrane was washed with TBST and incubated with appropriate secondary antibodies (horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG), followed by enhanced chemiluminescence (ECL; Visual protein, Taipei, Taiwan) and the membrane was examined by ECL spectrophotometry (Perkin Elmer, Geliance 600 Camera Imaging system ...
-
bioRxiv - Plant Biology 2020Quote: ... 10.5 μg of gDNA was digested with the restriction enzymes EcoRV and XbaI from New England Biolabs (http://www.neb.com) and blotted onto a nylon membrane (GeneScreenPlus; Perkin Elmer, http://www.perkinelmer.com) according to the manufacturer’s instructions.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were separated on 7% tris-glycine or 8% tricine gels by SDS-PAGE and transferred to a PVDF membrane (Perkin Elmer). The proteins were revealed using a V5-monoclonal antibody (V5-mAb V2660 ...
-
bioRxiv - Molecular Biology 2021Quote: ... total RNA was separated in 2% glyoxal agarose gels and blotted onto a positively charged nylon membrane (GeneScreen Plus, Perkin Elmer). [32P]-labelled probes specific for each transcript were generated by linear PCR ...
-
bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
-
bioRxiv - Microbiology 2022Quote: ... Samples were separated by SDS-PAGE using 10% or 15% (w/v) polyacrylamide gels and transferred to Protran nitrocellulose membranes (Perkin Elmer) using the Mini-Trans blot systems (BioRad ...
-
bioRxiv - Microbiology 2024Quote: ... Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer). OD600 of cell-free medium was removed from all cell density values ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
-
bioRxiv - Genetics 2023Quote: ... DNA was extracted from oral mucosal tissue using a commercial kit (chemagic™ DNA Buccal Swab Kit, PerkinElmer and DNAdvance Kit, Beckman Coulter). We performed real-time PCR to determine genotypes of DM-associated mutations (SOD1:c.118G
-
bioRxiv - Cell Biology 2020Quote: ... Luciferase activity measurement was performed using the Steadylite plus™ High Sensitivity Luminescence Reporter Gene Assay System (PerkinElmer). β-galactosidase activity measurement was performed using the Galacto-Light Plus system (Applied Biosystems).
-
bioRxiv - Neuroscience 2021Quote: ... 100 µL of 2% ACN/0.1% TFA was added to the reaction mixture and the mixture was analyzed by mobility shift assay (LabChip EZ Reader II and operation software version 2.2.126.0; PerkinElmer, U.S.A.).
-
bioRxiv - Cancer Biology 2021Quote: ... For the Alamar Blue assay and fluorescence emission was read on an EnVision 2101 multilabel plate reader (PerkinElmer; excitation = 540 nm ...
-
bioRxiv - Genomics 2021Quote: ... RNA quality was determined via the RNA Pico Sensitivity Assay on a LabChip GXII Touch (PerkinElmer, Waltham, Massachusetts).
-
bioRxiv - Pathology 2021Quote: – For palmitate oxidation assay: Cells were preincubated for 3 hours with 14Cpalmitate (1uCi/mL; Perkin Elmer, Boston MA) and non labeled (cold ...
-
bioRxiv - Cancer Biology 2021Quote: Protein synthesis assays were performed by measurement of the incorporation of 3H-Leucine (Perkin Elmer, Waltham, MA USA) into protein ...
-
bioRxiv - Microbiology 2023Quote: ... ATP production was measured as an indicator of viability using ATPlite™ Luminescence ATP Detection Assay System (PerkinElmer,). Luminescence was read using BioTek Synergy Hybrid Multi-Mode reader (BioTek,) ...
-
bioRxiv - Immunology 2023Quote: ... Libraries were QC’ed using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer, Waltham, MA). Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... All the assays were conducted at either 25 or 37°C in white 384-well Optiplate plates (PerkinElmer) using the PHERAstar FSX microplate reader (BMG Labtech) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 14C content was determined by direct DPM assay using a beta counter (TriCarb liquid scintillation counter, Perkin Elmer) and normalised to total protein.
-
bioRxiv - Physiology 2020Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols(Wang et al. ...
-
bioRxiv - Biochemistry 2022Quote: ... (PerkinElmer AlphaScreen Histidine Detection Kit) were added under low light conditions ...