Labshake search
Citations for PerkinElmer :
451 - 500 of 1032 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Immunology 2022Quote: ... and interleukin 10 (IL-10) were quantified in the supernatant from the PBMCs using an AlphaLISA assay (PerkinElmer, Waltham, MA). AlphaPlates containing 2 µL of supernatant were thawed at 25°C for 15 minutes ...
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bioRxiv - Plant Biology 2024Quote: ROS production was monitored by the use of a luminol-dependent assay as previously described (Butenko et al., 2014) using a Wallac 1420 VICTOR2 microplate luminometer (PerkinElmer). Arabidopsis leaves expressing 35S:HAE:YFP were cut into leaf discs and incubated in water overnight before measurements ...
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bioRxiv - Bioengineering 2023Quote: ... RNA integrity was analyzed using automated capillary electrophoresis (RNA Pico Sensitivity Assay, LabChip GX II Touch HT instrument, Perkin Elmer). Samples were stored at –80°C until further analysis.
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bioRxiv - Immunology 2022Quote: ... using the RNA Assay and DNA 5K/RNA/CZE HT Chip on a LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer). TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 48 hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo Luciferase Assay buffer containing Nano-Glo Luciferase Assay substrate diluted 1:200 and luminescence was measured after 3 minutes on an IVIS Specturum CT (PerkinElmer). To monitor merosome detachment at 65 hpi ...
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bioRxiv - Systems Biology 2022Quote: ... All ELISA and colorimetric assays were performed according to manufacturer’s instructions in combination with a microplate reader (VICTOR3 Multilabel Plate Reader, Perkin Elmer).
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bioRxiv - Microbiology 2023Quote: ... The tissue lysates were mixed with an equal volume of Nano-GLO Luciferase Assay Reagent (Nanolight) and luciferase activity was quantified on an Envision 2103 plate reader (PerkinElmer). Decoy concentration and half-life were determined based on a standard curve derived from the mixture of serial diluted ACE2.1mb-nLuc proteins and tissue lysate from wild-type ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μM [35S]GTPγS (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
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bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05□μl of 5□mM stock compounds in DMSO were dispensed into assay microplates with a Janus 384 MDT NanoHead (PerkinElmer). Final concentration of the screening compounds in the assay was 16LμM ...
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bioRxiv - Genetics 2024Quote: ... were directly loaded into wells of white strip plates prefilled with 200 µL of Assay Buffer (Perkin Elmer, Waltham, Massachusetts). Finally ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with horseradish peroxidase-conjugated IgG as a secondary antibody and the Western Lightning®-ECL system (PerkinElmer Inc., Waltham, MA) for detection.
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bioRxiv - Genetics 2024Quote: DNA was extracted from oral mucosal tissue using commercial kits (chemagic™ DNA Buccal Swab Kit, PerkinElmer, Waltham ...
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bioRxiv - Cell Biology 2020Quote: ... Lysis and luciferase activity detection was performed using the Gaussia-Juice Luciferase Assay (PJK Biotech, Kleinblittersdorf, Germany) according to the manufacturer’s description in white opaque 96-well plates (OptiPlate, PerkinElmer, Waltham, MA) and using a Synergy Mx microplate reader (BioTek ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Neuroscience 2021Quote: ... 100 µL of 2% ACN/0.1% TFA was added to the reaction mixture and the mixture was analyzed by mobility shift assay (LabChip EZ Reader II; PerkinElmer, U.S.A.). The kinase activity is the percentage of phosphorylated peptide signal over the total substrate peptide signal ...
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bioRxiv - Biophysics 2021Quote: ... Validation assays for 5-HT5AR-Gi1 expression were performed as described above using 10nM [3H]-LSD (82.4 Ci/mmol, PerkinElmer, Waltham, MA).
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bioRxiv - Cell Biology 2022Quote: ... An equal volume of cell culture media and esterase substrate (100 μM in esterase assay media at pH 5) was transferred to a black walled clear bottomed plate (Perkin Elmer) and fluorescence was measured after 1 h.
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bioRxiv - Physiology 2020Quote: ... The total concentration and purity of resulting RNA was determined using the RNA Pico Sensitivity Assay (Perkin Elmer, Cat. No. CLS960012) as per manufacturer’s instructions and loaded onto the DNA 5K/RNA/Charge Variant Assay LabChip (Perkin Elmer ...
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bioRxiv - Biochemistry 2022Quote: Michaelis-Menten kinetics of the untagged purified MtCM variants were determined by a continuous spectroscopic chorismate depletion assay (Lambda 20 UV/VIS spectrophotometer, Perkin Elmer). The enzymes were diluted to working stock solutions in 20 mM potassium phosphate ...
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bioRxiv - Cancer Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured using the Twinlite dual luciferase reporter gene assay system (Perkin Elmer, Wellesley, MA, USA) on an automated TECAN luminometer (TECAN Group Ltd ...
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bioRxiv - Microbiology 2022Quote: ... and serum IgA antibodies to the secreted form of S-2P were measured by europium ion-enhanced DELFIA-TRF assay (Perkin Elmer) following the supplier’s protocol.
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... the Her2 VHH library was resuspended in 500 µL Triton X-100 based stain buffer (AlphaLISA immunoassay assay buffer from Perkin Elmer; 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... half the medium was removed from all wells and cells were lysed by the addition of 50 µl of BriteLite assay readout solution (Perkin Elmer) to make a a 1:1 dilution into each well ...
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bioRxiv - Immunology 2023Quote: ... Libraries were assessed for concentration and fragment size using the DNA High Sensitivity Assay on the LabChip GX Touch (Perkin Elmer). The resulting libraries were sequenced on a multiplexed paired end 100bp Illumina NovaSeq 6000 S4 flowcell ...
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bioRxiv - Neuroscience 2023Quote: ... Horseradish peroxidase activity was visualized using an enhanced chemiluminescence assay in a Western Lightning Plus system (Perkin Elmer, Waltham, MA, USA). Chemiluminescent imaging was performed on a FUSION FX system (Vilber ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Plasma Lithium was measured using Inductively Coupled Plasma Mass Spectrometry assay (NexION 2000™ spectrometer, Perkin Elmer, Villebon-sur-Yvette, France). At the end of the protocol (6 months) ...
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bioRxiv - Molecular Biology 2023Quote: ... After 24h the luciferase assay was performed in white microplates and luminescence was measured with the EnVision 2104 Multilable plate reader (Perkin Elmer). GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm-pore size filter and quantified for viral Cap24 content using enzyme-linked immunosorbent assay (ELISA) (Perkin Elmer, # NEK050A) as previously published (41 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: V2R binding studies were based on time-resolved fluorescence resonance energy transfer (TR- FRET) measurements using Tag-Lite assays (PerkinElmer Revvity) as previously described19,34,51 ...
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bioRxiv - Genomics 2024Quote: ... DNA concentration and fragment size was conducted using the DNA High Sensitivity Assay on the LabChip GX (Perkin Elmer, Waltham, MA) tool ...
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bioRxiv - Systems Biology 2021Quote: ... Imaging was performed by HRP luminescence using Femto kit (ThermoFischer Scientific) or Western Lightning PlusECL kit (Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... Chemiluminescence was determined in a Fusion® SL chemiluminescence reader (Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany) from membranes incubated in Western Lightening® Plus-ECL solution (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... and TSA Plus Fluorescein amplification kit (PerkinElmer) for 1 hour and 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... amplified using TSA Plus Biotin kit (PerkinElmer) and visualized with Alexa Fluor 488-conjugated streptavidin (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... amplified using TSA Plus DIG kit (PerkinElmer) and visualized with DyLight 594-conjugated anti-DIG antibody (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: The Lance Ultra cAMP kit (Perkin Elmer) was used to measure the effect of unacylated ghrelin on cAMP production ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with tyramide (TSA kit, PerkinElmer) for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Enhanced Chemiluminescence Substrate kit (PerkinElmer ref. NEL103E001EA). The percentage of m6A in the main “band” co-migrating with the 2 kb molecular weight standard was measured by the intensity of the “band” divided by the signal intensity of the whole lane.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the LANCE Ultra cAMP kit (Perkin Elmer) was used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... using the ATPLite kit (6016943; Perkin Elmer), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... TSA Plus Fluorescence Kit (Perkin Elmer, # NEL741B001KT) was used for signal amplification ...
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bioRxiv - Cell Biology 2023Quote: ... the cAMP-Gi kit (Perkin Elmer, TRF0263) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...
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bioRxiv - Biochemistry 2022Quote: ... 48 hours post infection 50 µL of medium was removed from all cells were lysed by the addition of 50 µL BriteLite™ assay readout solution (Perkin Elmer) into each well ...
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Cell Biology 2021Quote: ... The ATPLite™ assay followed standard recommended no wash one step protocol and was read on an EnSight™ Multimode Plate Reader (PerkinElmer), without any deviation from the manufacturers protocol ...