Labshake search
Citations for PerkinElmer :
3001 - 3046 of 3046 citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Virus-mAb/plasma-containing media was then aspirated from cells and 100 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Neuroscience 2019Quote: ... The secondary and Opal™ steps were then repeated using the anti-rabbit secondary and Opal™ 650 reagent (1:1000; PerkinElmer). Sections were then washed 3x for 2 min each in TBS-T and counterstained with DAPI to label nuclei.
-
bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
-
bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
-
bioRxiv - Bioengineering 2022Quote: ... permeabilized for 30 min in 0.5% Triton X-100 in PBS and stained overnight with 1 μmol/mL CNA35-OG488 [30] and 0.2 nmol/ml OsteoSenseTM 680 (NEV10020EX, PerkinElmer, Waltham, MA, USA) at 4 ºC to visualize collagen and hydroxyapatite ...
-
bioRxiv - Biochemistry 2022Quote: ... Detection reagents were added stepwise with 30 min incubation of anti-H3K4me3 antibody and Ulight-Streptavidin with reaction time points followed by 1 hr incubation with Europium anti-rabbit antibody in 384 well white microplates (PerkinElmer OptiPlate-384). TR-FRET emission at 665 nm and at 615 nm by 320 nm excitation with 50 μs delay and 100 μs integration time was measured using a Molecular Devices SpectraMax M5e plate reader ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:3 dilution steps) using a Janus Gripper with 384-channel Modular Dispense Technology dispensing head (revvity, previously Perkin Elmer, MA, USA). Afterwards ...
-
bioRxiv - Microbiology 2023Quote: Prokaryotic (bacteria and archaea) heterotrophic production was estimated using the 3H-leucine incorporation method (Perkin Elmer, specific activity 100 Ci mmol−1). Water samples (1.7 mL each ...
-
bioRxiv - Biochemistry 2023Quote: ... Reactions were initiated by adding an equal volume of assay buffer containing 1 μm [γ-32P]GTP (~50 cpm/fmol, Perkin Elmer) at 30 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... BRB80 was mixed with a 1/10 volume of 10X nucleotide exchange buffer (10 mM GTP, 30 nM GTP α-32P (PerkinElmer BLU506H250UC), 1 mM nocodazole ...
-
bioRxiv - Molecular Biology 2023Quote: ... GLuc production was measured as a surrogate for IFN-β promoter activation by adding 25 µl coelenterazine-solution (1 µg/ml in H2O) was to 25 µl supernatant.reader (Perkin Elmer, Waltham, USA) and luminescence was measured ...
-
bioRxiv - Cancer Biology 2023Quote: ... The amount of eATP was analyzed according to the protocol described by the manufacturer of ATPlite 1-step Luminescence Assay System (PerkinElmer, Cat# 6016736) either at 0 hours (eATP concentrations were determined immediately ...
-
bioRxiv - Cell Biology 2024Quote: ... we used the following secondary antibodies: HRP-labeled goat anti-rabbit and mouse IgG (1:10000, NEF812 and NEF822, PerkinElmer, Waltham, MA). For immunofluorescence ...
-
Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with Hoechst (1/5000) for 30 min and fluorescence was monitored using victor x4 2030 Multilabel Reader (PerkinElmer, Waltham, MA, USA) with excitation wavelength at 350 nm ...
-
bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... All the videos (2 min, 4 frames s-1) were acquired at 37 °C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
-
bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Oxaliplatin loaded Cedrus libani Sporopollenin (CD-SP-OX) were analyzed in the range of 4000-650 cm-1 using an FT-IR spectrometer (Perkin Elmer 100, USA) under ambient conditions.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
-
bioRxiv - Cell Biology 2020Quote: In vitro kinase reactions were developed in a final volume of 50 μl containing 0.02 mM [γ-32P]ATP (1 µCi per tube, Perkin Elmer, Massachusetts, USA), 50 mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were washed again in PBS with 0.1% Tween-20 six times for 1 hour and incubated in fluorescein tyramide reagent (Perkin Elmer, Cat. No. SAT701001EA) diluted 1:70 in amplification diluent for 50 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... They were stored in PBS + 0.1% w/v sodium azide before imaging on a X-ray micro-CT device (Quantum FX Caliper, Life Sciences, Perkin Elmer, Waltham, MA) hosted by the PIV Platform ...
-
bioRxiv - Cancer Biology 2024Quote: ... then separated in an 1% formaldehyde-containing agarose gel and transferred onto a GeneScreen Plus hybridization transfer membrane (Perkin Elmer, Waltham, MA, #NEF987001PK). RNAs on the membrane were crosslinked by exposing to UV light ...
-
bioRxiv - Cell Biology 2023Quote: All the videos (2 min, 4-5 frames s-1) were acquired at 37°C using a Nikon TIRF microscopy system (Perkin Elmer, Waltham, MA) on an inverted Ti microscope equipped with a 100× objective and an ImageEM C9100-13 camera (Hamamatsu Photonics ...
-
bioRxiv - Biochemistry 2024Quote: ... The lysed cells were diluted 1:2 with assay buffer after which 10 μL/well was pipetted to a white 384-well OptiPlate (PerkinElmer, Waltham, MA, USA). Next ...
-
bioRxiv - Immunology 2021Quote: ... Plasma 2-[1-14C]-Deoxy-D-glucose radioactivity was directly measured in a liquid scintillation counter (Tri-Carb 2810 TR, Perkin Elmer, Waltham, MA, USA). Tissue lysates were processed through ion exchange chromatography columns (Bio Rad Laboratories Inc. ...
-
bioRxiv - Microbiology 2021Quote: ... in a ratio of 5:95 and analyzed at the mid-IR region (400-4,000 cm-1) of the spectrometer (Perkin Elmer Spectrum 100, United States). The biodegraded products of AB1 were identified using LC-MS analyses (Dionex Ultimate 3000 ...
-
bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated for 10 min in Cy3 Plus Amplification Reagent diluted 1:300 in 1x Plus Amplification Diluent (TSA Plus Cyanine 3 System, Perkin Elmer, Waltham, MA, NEL744001KT), washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
-
bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at room temperature with alkaline phosphatase-coupled goat antirabbit antibodies and developed with Western Lightning Plus (Perkin Elmer, Inc., Waltham, MA) according to manufacturer directions.
-
bioRxiv - Cell Biology 2023Quote: ... Spectrum Spotlight 400 FT-IR Imaging system was used to acquire spatial resolution images in the mid-IR region from 4000 to 800LJcm−1 (Perkin Elmer, Waltham, MA, USA) of three consecutive sections/disc to minimize section-based variation ...
-
bioRxiv - Cancer Biology 2024Quote: ... The tumor burden was quantified weekly by bioluminescent signal intensity on isoflurane-anesthetized mice that received a subcutaneous injection of D-luciferin (150 μg luciferin/1 g mouse body weight) 10 min prior to IVIS® imaging (PerkinElmer, Waltham, MA,USA). Survival data were presented and reported in Kaplan–Meier plots.39
-
bioRxiv - Biophysics 2019Quote: ... The solubilized virus was diluted in HB buffer with 1% Triton-X and the fluorescence emission spectrum of the sample was measured using a fluorimeter (Perkin Elmer LS 55, Waltham, MA). The integrated fluorescence intensity across the spectrum was calculated and compared to a standard curve of known dye concentration in the same buffer to determine the concentration of fluorescent dye in the viral suspension ...
-
bioRxiv - Genetics 2019Quote: ... were mounted on infrared windows where spectral images were collected at a 4 cm-1 spectral resolution and ~7 μm spatial resolution from a Spotlight 400 Imaging system (Perkin Elmer Instruments, Shelton, CT USA). Background spectra were collected under identical conditions from clear Ba2F windows and subtracted from sample data by instrumental software ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences) and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-mouse Chd7 primary antibody was followed by signal amplification with goat anti-rabbit IgG horseradish peroxidase (1: 500) (Perkin Elmer Inc: NEF812001EA, Waltham, MA). The tyramide signal amplification plus Cy3 Kit (1:1500 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit conjugated to peroxidase (Perkin Elmer and Sigma, Aldrich respectively). The latter were developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences).
-
bioRxiv - Developmental Biology 2024Quote: ... The heads were rinsed five times in PBST at room temperature and then incubated in 50x diluted Cy3 Tyramide Reagent (PerkinElmer Life Science, Inc. 1:50 dilution) in Amplification Dilution buffer for 90 minutes at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... Images were acquired for 60 min at 1 frame/15-30 s using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective). Images were analyzed using Volocity Suite (PerkinElmer ...
-
bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
-
bioRxiv - Microbiology 2023Quote: ... collected water samples (45 ml in 50 ml Falcon tubes, with small aerobic headspace) were spiked with 5 µCi of NaH14CO3 (Perkin Elmer, specific activity 56 mCi mmol−1). The samples were incubated for 24 h at ∼ 20 °C in the dark ...