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Citations for PerkinElmer :
2851 - 2900 of 3046 citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-and glucose-free DMEM for 2 hrs before a 3-hr incubation in low-glucose DMEM/fatty acid-free BSA 0.2% supplemented with radiolabeled glucose (Glucose, D-[3-3H]; 1 μCi/ml; PerkinElmer). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer) confocal screening microscope.
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bioRxiv - Molecular Biology 2021Quote: ... The medium was removed and 125I-GLP-1(7-36)NH2 (60 pM) or 125I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled tirzepatide or peptide 20 were added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in a goat anti-rabbit horse radish peroxidase-conjugated IgG (1:200, NEF812001EA, PerkinElmer, Waltham, MA) for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Two 100-μl fractions were collected in 50 μl of 1.5 M Tris-HCl (pH 8.0) and 1 ml of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity was measured using a liquid scintillation analyser (Packard).
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Bioengineering 2021Quote: ... background luminescence was measured for each rat (1 sec exposure, field of view D, PerkinElmer IVIS, Waltham, MA, USA). Both knees were then injected with either 50 μL NanoLuc™ Luciferase (NL ...
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide (1:50; SAT701001KT; Tyramide Signal Amplification FITC Systems, PerkinElmer) for 3 min ...
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bioRxiv - Immunology 2023Quote: ... Samples were incubated in triplicate (106 per sample) with 1 µCi of 2-[1,2-3H]-deoxyglucose (20 Ci/mmol; PerkinElmer) in uptake buffer for 4 min at room temperature and immediately spun through a layer of bromo-dodecane into a layer of 20% perchloric acid/8% sucrose to stop the reaction and separate cells from unincorporated 2-[3H]-deoxyglucose ...
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bioRxiv - Microbiology 2024Quote: The lipid composition of bacterial strains was determined after labeling with [1-14C] acetate (60 mCi/mmol; Perkin Elmer), [32P] phosphate (1 Ci/mmol ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5/1 μM fluoxetine) containing 50 pM of the DAT ligand (125I) RTI55 (PerkinElmer Life Sciences, Boston, USA) for 60 min ...
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bioRxiv - Plant Biology 2023Quote: ... samples were incubated for 1h in 1:50 of a digoxigenin-tyramide signal amplification solution (TSA Plus DIG, PerkinElmer) prepared according to manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed 3 times with wash buffer (PBS + 1 mM CaCl2 + 0.1% BSA) before adding Microscint-20 (PerkinElmer) for tritium concentration measurement using a TriLux MicroBeta2 plate reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 37 °C and imaged with the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). Number of counted nuclei was determined with the image analysis software Harmony (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then separated on a HayeSep column (7’ HayeSep N 1/8’’ Sf; PerkinElmer Inc., Waltham, USA), followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf ...
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bioRxiv - Cancer Biology 2023Quote: ... 4T-1-Luc3 tumors and metastases were monitored by bioluminescence imaging on an IVIS Lumina III System (Perkin Elmer) twice a week after tumor implantation ...
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bioRxiv - Biochemistry 2023Quote: ... 100 nM of WT FCE or mutant K286M was incubated with 1 μM of 32P-α-GTP (Perkin Elmer) in a 20 μL reaction containing 1x RNA capping buffer at room temperature for 1 minute ...
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bioRxiv - Bioengineering 2023Quote: We used hairless SKH-1 mice for live animal imaging on IVIS Spectrum In vivo Imaging System (Perkin Elmer). For ovalbumin quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... was added to the media at a 1:10,000 dilution and images were acquired using the Opera Phenix High-Content Screening System (PerkinElmer). Analysis was performed using Harmony 191 High-Content Imaging and Analysis Software (PerkinElmer ...
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bioRxiv - Cancer Biology 2021Quote: In vitro methyltransferase assays were performed using 30 ul reaction buffer (50 mM Tris-HCl pH 7.8, 50 mM KCl, 5 mM MgCl2) with 1 ul 3H-AdoMet (Perkin Elmer, specifific activity = 55-85 Ci/mMole,0.55 μCi/ul ...
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the reactions were started by adding 5× NTP mixture (1 mM for ATP, CTP and GTP, 0.1 mM for UTP) containing 2 µCi of [α−32P] UTP (PerkinElmer). Transcription reactions were performed at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 nmol of chemerin-9 in 25 μL iodination buffer (0.5 M sodium phosphate, pH 7.4) were mixed with 1 mCi carrier-free Na125I (NEZ033L010MC, PerkinElmer, Waltham, USA) in an HPLC glass vial with microvolume insert ...
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bioRxiv - Biochemistry 2021Quote: ... The filter paper was air dried for 1 hour and then placed into 2 ml of scintillation liquid (Ultima Gold, PerkinElmer), and radioactivity was counted using a scintillation counter (Tri-Carb B2810TR ...
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bioRxiv - Bioengineering 2020Quote: ... Azide functionalization was confirmed with Fourier-transform infrared spectroscopy (FTIR, Supplemental Figure 1, F Perkin Elmer Frontier MIR/NIR spectrometer) and quantified with electrospray ionization-mass spectrometry (ESI-MS ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM MgCl2 and 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc; Perkin Elmer) in 15μl reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse (1:5000) were used and detected by using the enhance Chemiluminescent Substrate (PerkinElmer). The bands were visualized using the ChemiDoc Imaging System (BioRad).
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bioRxiv - Molecular Biology 2022Quote: Undifferentiated H1-OCT4-GFP-INS:tdT-luciferase hPSC and the differentiated β-like cells were incubated with 15mg/mL D-luciferin for 1 min before imaging with an IVIS Spectrum system (PerkinElmer), with an exposure of 1 min and mid-bin setting ...
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bioRxiv - Neuroscience 2022Quote: ... 022-105721-00321-0001, POLATOM, as a marker for K+ transport, and 4 μCi ml-1 3H-mannitol as an extracellular marker, PerkinElmer) for 2min (influx ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were washed in PBSTw and then incubated in FITC-Tyramide (Perkin-Elmer) 1:100 in amplification reagent (Perkin Elmer), followed by several washes in PBSTw ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Neuroscience 2020Quote: Larval stage 1 animals (L1) were imaged in a UltraView VoX spinning-disc confocal microscope (PerkinElmer Life and Analytical Sciences). Embryonic imaging was performed via dual-view inverted light sheet microscopy (diSPIM)64,65 ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Cell Biology 2019Quote: A centrifuge-free RABiT-II system was used for automated sample processing after culturing: The system consists of two part: the first (Figure 1) has an automated liquid-handling system (JANUS, PerkinElmer) with integrated automated microplate handler (Twister3 ...
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bioRxiv - Plant Biology 2020Quote: ... or without (for pPcUBI (I) and (II)) 1 μM Brassinazole medium were imaged using an Ultra View Vox Spinning disc confocal imaging system (PerkinElmer) mounted on an Eclipse Ti inverted microscope (Nikon ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 20 μl of the reaction mixture was incubated at 26°C for 1 h in a 384-well AlphaPlate (PerkinElmer). Subsequently ...