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Citations for PerkinElmer :
201 - 250 of 854 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The 96-well plate was incubated for 24h at 37 °C in a Victor3 microtiter plate reader (PerkinElmer, Waltham, MA, USA), and absorbance was measured every three minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Bioluminescence was recorded every 30 min in DD at 25°C and 65% humidity using a TopCount Multiplate Reader (Perkin Elmer). An sCMOS camera (DMK33UX178 ...
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bioRxiv - Biochemistry 2023Quote: ... Filter-bound [3H]VUF15485 was measured by adding 25 µL Microscint-O per well to the dried GF/C-plate and radioactivity was consecutively quantified using the Wallac Microbeta counter (Perkin Elmer). The sigmoidal dose-dependent displacement curves of [3H]VUF15485 binding by unlabelled ligands were analysed using Graphpad Prism 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then maintained at constant 37°C for 36 hours before moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Biophysics 2023Quote: ... and their proliferation was recorded at 37 °C with a Nikon Ti inverted microscope using an Ultraview spinning disk confocal system (Perkin Elmer). For determining TEM opening ...
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bioRxiv - Biochemistry 2021Quote: Cellular and mitochondrial copper levels were measured by inductively coupled plasma (ICP) mass spectrometry using NexION 300D instrument from PerkinElmer Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were carefully added and plates were incubated for 10 min before luminescence reading in the EnVision 2103 Multilabel Reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... Plates were incubated with lids in place for 15 min at room temperature and then luminescence readings were taken using a ViewLux ultra HTS Microplate Imager (Perkin Elmer) with a 2 second exposure time per plate ...
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bioRxiv - Cancer Biology 2024Quote: ... for 72 h followed by reading the screen using a storage phosphor system (Cyclone Plus, PerkinElmer™, Waltham, MA, United States). The detected activity ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM FAM-12-dCTP (Perkin Elmer) and 0.5 U/µL DNA Polymerase I Large (Klenow ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Cell Biology 2022Quote: PDE activity was measured at 30°C with the two-step method described by [48] using [3H]cGMP (Perkin Elmer, MA, USA). Aliquots of eluted fraction from SEC were incubated in 60 mM HEPES pH 7.2 assay buffer containing 0.1 mM EGTA ...
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bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
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bioRxiv - Immunology 2020Quote: ... The pyroptotic process was monitored at 34°C and 7.5% CO2 using a fully automated spinning disk confocal microscope (PerkinElmer Technologies, OPERA QEHS) with a 40x water immersion objective (Aulner et al. ...
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bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
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bioRxiv - Plant Biology 2020Quote: ... The reaction was incubated for four hours at 37°C and the results were visualised using the LabChip GX Touch 24 (PerkinElmer, Waltham, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
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bioRxiv - Immunology 2022Quote: ... and the reaction was continued for 24 hours at 37 °C before FP measurement using a Victor X5 Multilabel plate reader (PerkinElmer, Shelton, CT). DP4.1-Clip (250 nM) ...
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bioRxiv - Microbiology 2023Quote: ... in 96-well plates in 28°C with shaking and the OD600 measurements were performed hourly using an EnVision Multilabel Reader (Perkin Elmer, USA).
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bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...
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bioRxiv - Molecular Biology 2024Quote: ... This RNA unwinding assay was performed at 37 °C in 384-well plates using a Victor Nivo plate reader (PerkinElmer, Basel, Switzerland). The excitation wavelength was set to 640 nM and the emission was measured at 685 nm ...
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bioRxiv - Cell Biology 2020Quote: ... solid-state laser lines and a cooled 14-bit frame transfer EMCCD C9100-50 camera (Hamamatsu) and is run by Volocity (PerkinElmer). When imaging strains expressing the CRY2-Cdc42ΔCaaX and/or CRY2 systems ...
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bioRxiv - Cell Biology 2019Quote: ... solid-state laser lines and a cooled 14-bit frame transfer EMCCD C9100-50 camera (Hamamatsu) and is run by Volocity (PerkinElmer). When imaging strains expressing the OptoQ61L and/or Opto systems ...
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bioRxiv - Cell Biology 2020Quote: ... was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer, USA). Measurements were done at least in triplicates and relative luciferase units (RLU ...
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bioRxiv - Microbiology 2022Quote: ... β-galactosidase activity was measured by adding 50 μL/well of a 4.8 mg/mL solution of chlorophenol red-β-D-galactopyranoside (CPRG; Boehringer Mannheim) and reading the absorbance at 560 nm on a plate reader (Perkin Elmer). This assay was repeated at least three times.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40 µL per well of Nano-Glo reagent was added and the plates were incubated at room temperature for 30 min before reading the luminescence signal on an EnVision multimode plate reader (PerkinElmer, Waltham, MA). Compound 1 was used as positive control and DMSO was used as negative control ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was incubated at 37 °C for 20 min before the RFU of fluorescent signals were read on an Envision microplate reader (Perkin Elmer, Waltham, MA) with excitation wavelength set at 320 nm and emission wavelength set at 400 nm ...
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bioRxiv - Physiology 2020Quote: ... Progesterone was extracted with ethyl ether after overnight equilibration (4°C) of the plasma with 1500 dpm of tritiated progesterone (Perkin Elmer, Rodgau, Ermany). Separation of the organic and aqueous phase was conducted similar to the procedure described for the other hormones ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Donor and acceptor emission were measured at 3 min intervals (during a total of 66 min) at 37°C with an EnVision 2014 Multilabel Reader (PerkinElmer, Waltham, MA, USA) using a 340/60 nm excitation filter and 520/8 nm (acceptor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... lung tissues harvested from euthanized mice were soaked for 10 min in pre-warmed (37°C) PBS with 2% FBS and a 1:10 dilution of Xenolight RediJect Coelenterazine h (Cat# 706506, Perkin Elmer, Waltham, MA). Following a 12-15 min incubation ...
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bioRxiv - Microbiology 2020Quote: ... Amastigotes were incubated at 37°C for 72 hours followed by lysis and measurement of luminescence using the ATPlite assay (Perkin Elmer, Waltham, MA).
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bioRxiv - Microbiology 2023Quote: ... was added to bacterial supernatants and the enzymatic activity was monitored for 30 min at 37°C (380 nm) in a VICTOR2 fluorometer/spectrophotometer (Perkin Elmer, Waltham, MA). The enzymatic activity ([PNA generated (nM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... DPBS samples were diluted in 2% HNO3 and plasma samples were digested over night at 70°C in the 1:1 mixture of 70% HNO3 and H2O2 and analyzed using ICP-MS (Perkin Elmer NexION 300D) to determine the concentration of silver within each sample ...
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bioRxiv - Biochemistry 2024Quote: ... 2B were prepared in ICM (Molsoft LLC., San Diego, CA). Chemical structures (Fig. 5A, C) were generated using ChemDraw (PerkinElmer informatics, Waltham, MA).
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bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP, Perkin Elmer, 1:200 dilution) followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were incubated with a mixture of 30 μM of GABA and [3H] GABA (40 μCi, specific activity = 25 Ci / mmol, PerkinElmer, 15 min, 37 ° C.). The supernatant is then removed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plate was incubated in the dark for 60 min at room temperature (∼22 °C) and subsequently read on an EnVision Multilabel Reader (PerkinElmer Life and Analytical Sciences), with excitation at 340 nm and measurements of emission at 615 and 665 nm ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
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bioRxiv - Immunology 2023Quote: ... A chemiluminescence assay was conducted for continuous measurement of chemiluminescence counts per second (cps) at 37°C in an Envision multimode plate reader (PerkinElmer Life Sciences, Waltham, MA), recording measurements at 1 min intervals.
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bioRxiv - Synthetic Biology 2019Quote: ... and fluorescein-12-ddUTP (Perkin Elmer, San Jose, CA) by incubating 0.1mg of beads with 5μM nucleotide and 20U of enzyme in 20mM Tris-HCl pH7.5 ...