Labshake search
Citations for PerkinElmer :
151 - 200 of 854 citations for Human Mitochondrial Open Reading Frame Of The 12S rRNA c MOTS c CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The plate was sealed with parafilm and placed in a pre-heated (37°C) Opera Phenix microscope with 40x or 60x water-immersion lens (PerkinElmer) with 5% CO2.
-
bioRxiv - Molecular Biology 2022Quote: ... was next added and incubated with the mixture at 4 °C for 1 hour before the measurements performed on Envision Readers (PerkinElmer). The fluorescently-labeled m6A-containing mRNA was used to adjust the gain factor ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stored in PBS at 4°C until imaged in the Opera Phenix (PerkinElmer) at 40x magnification and images analysed using a Harmony software to assess protein expression and morphology (see supplementary).
-
bioRxiv - Bioengineering 2023Quote: ... of approximately 5.7 ± 3.0 pmol/mg protein measured by the manufacturer using [3H]-DAMGO (ES-542-C OP3 ValiScreen, PerkinElmer, Waltham, MA) were obtained and cultured for a series of binding assays ...
-
bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
-
bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
-
bioRxiv - Immunology 2023Quote: ... After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer) according to the manufacturer’s protocol.
-
bioRxiv - Genetics 2023Quote: ... Live imaging was performed with a Nikon TE2000 microscope at 37°C using Volocity software (Nikon Corporation, Tokyo, Japan; PerkinElmer). Images were captured with 488 nm excitation and 516 nm emission at a rate of 20 images per second for 30 seconds ...
-
bioRxiv - Neuroscience 2023Quote: ... Live cell imaging was performed at 37°C in 5% CO2 for 24 hours using the Opera Phenix High Content Screening System (PerkinElmer). Acquired images were analyzed by using the Harmony software (PerkinElmer).
-
bioRxiv - Cancer Biology 2024Quote: ... was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer). For longer-term proliferation assays ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were read once every 30 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 550 and 591 nm ...
-
bioRxiv - Microbiology 2023Quote: ... purified 6×His-tagged SfTIR-STING was added to the reaction to a final assay volume of 50 μL and plates were read once every 15 s for 10 min at 37°C using a EnSpire Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of 410 and 300 nm ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were blocked with 5% BSA in Tris / HCl-buffered saline / 0.05% Tween (TBST) for 24 h at 4 °C and afterwards membranes were incubated for 1 h with HRP-Streptavidin (PerkinElmer; #NEL750001EA ...
-
bioRxiv - Bioengineering 2024Quote: ... The plates were incubated for 4 h at 37°C and then the supernatant was removed and incubated with scintillation fluid (PerkinElmer) overnight at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer], 3H uridine [0.1 μCi, Perkin Elmer] or 3H thymidine [0.32 μCi ...
-
bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer] ...
-
bioRxiv - Cell Biology 2024Quote: Images of living embryos were acquired at 23°C using a PerkinElmer ERS spinning disk confocal system mounted on a Zeiss Axiovet 200M microscope using Volocity software (PerkinElmer). A 63X ...
-
bioRxiv - Neuroscience 2023Quote: ... Mitochondrial ROS was visualised using an OPERA high content imager (488 nm excitation, 530/30 nm emission) (Perkin Elmer).
-
bioRxiv - Physiology 2023Quote: ... Mitochondrial uptake into organs was determined ex vivo using epifluorescent bioimaging (Xenogen IVIS Lumina, Perkin Elmer Akron, OH, USA) at 30 min ...
-
bioRxiv - Genetics 2019Quote: ... and fluorescein-12-dUTP (Perkin Elmer), respectively ...
-
bioRxiv - Microbiology 2023Quote: ... A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
-
bioRxiv - Microbiology 2023Quote: ... Lipid peroxidation was assessed by measuring the change in fluorescence of C11-BODIPY at 488nm using direct fluorescence reading with the plate reader (Envision PerkinElmer).
-
bioRxiv - Biophysics 2019Quote: ... The plate was then incubated for 20-48 hours at 30°C in a Victor X3 multimode plate reader (Perkin Elmer). Every 10 minutes ...
-
bioRxiv - Cell Biology 2019Quote: ... Reactions were then rapidly filtered under vacuum through GF/C glass fiber filter-bottom 96-well microplates (Perkin Elmer, MA, USA) pre-treated with 0.1% polyethyleneimine (PEI ...
-
bioRxiv - Immunology 2020Quote: ... Plates were stored in the dark at 4°C prior to imaging using a 40x objective mounted on an Operetta High-Content Imaging System (Perkin Elmer) controlled by Harmony 4.1 software.
-
bioRxiv - Cell Biology 2019Quote: ... AMC fluorescence released by cleavage of substrate by proteolytic activity was recorded in a black 96-well plate after 60 min incubation at 37°C using the Victor 3V Multilabel counter (Perkin Elmer). Specific proteasomal activity was determined by subtracting unspecific AMC fluorescence obtained in the presence of proteasome inhibitor MG132 (20 μM) ...
-
bioRxiv - Biochemistry 2021Quote: ... Complexes were incubated for 1h at 37°C and luminescence released by the [14C]-RITA-excited beads was measured in standard microplate reader (Perkin Elmer).
-
bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
-
bioRxiv - Biochemistry 2021Quote: ... and mRNA was translated for 1 hour at 30 °C in presence of 10 µCi/µL EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). The mixtures were centrifuged for 2 min at 10,000 x g at 4 °C after inhibiting protein synthesis with 1 mM cycloheximide and translocated CFTR was retrieved from the pellet fraction ...
-
bioRxiv - Cell Biology 2020Quote: ... and recorded on a 37°C heated stage of Nikon Ti inverted microscope using Ultraview spinning disk confocal system (Perkin Elmer). For the TEM opening ...
-
bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
-
bioRxiv - Developmental Biology 2022Quote: ... For signal detection sections were incubated with the channel specific HRP for 20 min at 40 °C and incubated with the respective fluorophores (Perkin Elmer: Fluorescein ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
-
Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... were hybridized for 2 h at 40°C followed by signal amplification and developing the HRP channel with TSA Plus fluorophore Cyanine 3 (1:1500) (NEL744001KT, Perkin Elmer) according to the manual ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
-
bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
-
bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
-
bioRxiv - Neuroscience 2020Quote: ... Imaging was performed at 37°C and 3% CO2 using an Opera Phenix™ high-content confocal microscopy system (Perkin Elmer). Images were acquired at 40x magnification using a 425 nm laser for excitation of CFP and emissions detected at 435-515 nm (CFP ...
-
Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Microbiology 2021Quote: ... Liquid cultures were incubated for seven days at 29 °C in the dark and the bioluminescence signal was measured daily with an EnVision plate reader (Perkin Elmer) three times for three seconds per well ...
-
bioRxiv - Microbiology 2021Quote: ... 200 µl of the diluted cultures were transferred onto 96-well plate and OD490 at 37 °C was measured at fixed interval using Victor X4 plate reader (Perkin Elmer). For manual growth curve and CFU enumeration ...
-
bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 200 μL of these dilutions were then pipetted into opaque 96-well microplates and incubated at 30°C in a Victor X3 multilabel microplate reader (Perkin Elmer) that periodically measured absorbance (OD600 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
-
bioRxiv - Microbiology 2023Quote: Growth of bacterial cultures to study phage infection or toxicity of SAMase was carried at 37°C in a 96-well format using EnSpire Multimode Plate Reader (Perkin Elmer). Overnight bacterial cultures were diluted 100-fold in LB medium with appropriate antibiotics and grown at 37°C in 10 mL of LB ...
-
bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...