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Citations for PerkinElmer :
201 - 245 of 245 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Biophysics 2020Quote: ... 100 nM hNatB was mixed with a saturating concentration of “MDVF” peptide substrate (500 μM) and varying concentrations (1.95 μM to 1 mM) of acetyl-CoA (14C-labeled, 4 mCi mmol−1; PerkinElmer Life Sciences) for 10-minute reactions ...
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bioRxiv - Cell Biology 2020Quote: ... Glass fibre prefilters were air dried for 5 min and the amount of 35S-labeled methionine was analyzed with 5 ml liquid scintillation cocktail Ultima Gold™ (6013329, PerkinElmer) by Liquid Scintilation Analyzer Tri-Carb 4910 TR (PerkinElmer).
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Microbiology 2020Quote: ... cells were metabolically pulse labeled with methionine- and cystine-free DMEM supplemented with 70 μCi/ml 35S-labelled Met/Cys (EasyTag EXPRESS35S, Perkin Elmer). Total protein was boiled in SDS sample buffer and then either fractionated by SDS PAGE and exposed to film or precipitated in 10% Trichloroacetic acid (TCA ...
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bioRxiv - Microbiology 2020Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [a32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Cell Biology 2020Quote: ... washed twice with the same medium and pulse labeled for 15 min with 35S-Cys/Met (300 μCi, Cat. No. NEG072007MC, Perkin Elmer). After pulse ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Neuroscience 2023Quote: ... probes were fluorescently labeled with Opal Dyes (Opal 520 product no. FP1487001KT, Opal 570 product no. FP1488001KT, Opal 690 product no. FP1497001KT; Perkin Elmer). Autofluorescence from lipofuscin was quenched using the reagent TrueBlack (Biotium ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested after 48 hr transfection and nuclear extracts were prepared as previously described.78 The two strands of short probe was labeled with [γ-32P]-adenosine triphosphate (PerkinElmer). After that ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
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bioRxiv - Microbiology 2022Quote: ... Probes corresponding to the 5’ and 3’ flanks of the laeA gene in each strain were labeled using [α32P] dCTP (PerkinElmer, USA) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the supernatant was counted for [14C]-labeled acid-soluble β-oxidation products using a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer). These [14C]-labeled acid-soluble β-oxidation products are deemed to be mainly ketone bodies and to a lesser extent intermediates of the tricarboxylic acid cycle (Fromenty et al ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was counted for [14C]-labeled acid-soluble β-oxidation products using a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer). Results were normalized to cell number as described for oxygen consumption.
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bioRxiv - Cell Biology 2020Quote: ... Cells were pulse labeled for 1 hr with 120 μCi of EasyTag Express35S Protein Labeling Mix [35S]-Cys/Met (PerkinElmer; Waltham, MA). Immediately after the radioactive pulse ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The reverse primer R3 (5′-GCGCCGGTAGTTGCCCTTCTC-3′) was labeled at the 5′ end using [γ-32P]-ATP (PerkinElmer Inc., Waltham, MA, USA) and T4 polynucleotide kinase (Takara) ...
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer). The signal was normalized to the relative cellular content as determined by immunoblotting of whole cell lysates probed with antibodies.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells transfected with FTTTRA25T and were labeled for 30 min with EasyTag Express [35S] Protein Labeling Mix (0.1 mCi/mL; Perkin Elmer CAT# NEG772014MC) in DMEM lacking both Cys and Met (Gibco CAT# 21013024 ...
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bioRxiv - Molecular Biology 2020Quote: 100 pmol of WT or mutant RNA oligonucleotides (synthesized by Integrated DNA Technologies, Inc.) were 5’- end labeled with [γ32P] ATP (Perkin Elmer, NEG035C001MC) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM SpNatC (WT or mutants) was mixed with 50 μM Ac-CoA (14C-labeled, 4 mCi mmol-1; PerkinElmer Life Sciences) and varying peptide concentrations (ranging from 1.95 μM to 500 μM ...
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bioRxiv - Biochemistry 2021Quote: ... the R2R and primer extension (PE) DNA primers (100 pmol) were labeled with [γ-32P]ATP (125 pmol; 6,000 Ci/mmol; 150 μCi/ μl; PerkinElmer Life Sciences) by incubating the DNA with T4 polynucleotide kinase (10 units ...
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bioRxiv - Neuroscience 2021Quote: ... Biotin-labeled second-round aRNA was generated with an NTP-mix containing Biotin-11-CTP and Biotin-16-UTP (PerkinElmer, Boston, MA) at 2 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
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bioRxiv - Biophysics 2023Quote: ... stand and a 40 nt oligo-dT at the 5′ end was labeled at the 3′ terminus with [α-32P] dCTP (Perkin Elmer) and annealed to the single-stranded DNA M13mp18(24) ...
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bioRxiv - Cell Biology 2022Quote: ... Nascent proteins are labeled during translation reactions of 15-30 min at 32°C in RRL by incorporation of radioactive 35S-methionine (Perkin Elmer, USA). Nascent TA proteins were released from the ribosome with 1 mM puromycin and then incubated with 5% (v/v ...
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bioRxiv - Cell Biology 2024Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Microbiology 2024Quote: ... RNA transcripts from the ffh and zapA promoters were labeled with 2– 10 µCi of [α-32P] ATP or [α-32P] UTP (PerkinElmer), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... we used the following secondary antibodies: HRP-labeled goat anti-rabbit and mouse IgG (1:10000, NEF812 and NEF822, PerkinElmer, Waltham, MA). For immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Biotinylated human recombinant KRAS WT, G12C, G12D, and G12V proteins (Amid Bioscience, Santa Clara, CA) were pretreated with Terbium-labeled streptavidin (Tb-SA) (PerkinElmer, Waltham, MA) and BODIPY-FL-GDP (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Hairpin substrates with a two-nucleotide 5′-overhang end for EM-SAs were assembled by self-annealing oligonucleotides that were 5′-end labeled with [γ-32P]-ATP (Perkin Elmer) and T4 polynucleotide kinase (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... Transcripts were fluorescently labeled with Opal 520 (1:1500) and Opal 570 (1:500) using the Opal 7 Kit (Perkin Elmer; NEL797001KT). Images were acquired on a Zeiss LSM 880 inverted confocal system with 20X air and 40X oil objectives using Zen Black software (Carl Zeiss) ...
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bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
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bioRxiv - Biochemistry 2021Quote: ... alone or in the presence of 100 μM Na2S was assayed for its ability to autophosphorylate in a reaction containing 50 µCi of [γ-32P]-labeled ATP (6000 Ci/mmol, PerkinElmer Health Sciences), 100 mM Tris-HCl ...
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bioRxiv - Physiology 2022Quote: ... The assay was a mobility shift assay based on charge differences before and after electrophoretic separation of product from fluorescently labeled substrate read using a Caliper EZ Reader (Perkin Elmer, Boston, MA). All reactions took place in the presence of excess nicotinamide adenine dinucleotide and the known SIRT1 inhibitor suramin was used as a control.
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bioRxiv - Microbiology 2021Quote: ... SVC1-scramble and isogenic non-invasive SVC1 bacteria (lacking pSiVEC-scramble) were fluorescently labeled using the XenoLight RediJect 750 near-infrared fluorescent probe (Perkin Elmer, Waltham, MA). Nine-week-old female BALB/c mice (Jackson Laboratory ...
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bioRxiv - Molecular Biology 2022Quote: U10 and CUGCUGUUUU (20 pmol) were chemically synthesized (Integrated DNA Technologies (IDT)) and 20 pmol RNA was radioactively labeled using [γ-32P]-ATP (PerkinElmer, 10mCi/ml) and 10 units T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Plant Biology 2024Quote: ... Assignment of proposed structures was performed by reference to labeled standards and analysis of the fragmentation spectra using ChemDraw (PerkinElmer, Waltham, Massachusetts, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [U-14C] labeled acid-soluble β-oxidation products were measured in a Tri-Carb 4910TR liquid scintillation counter (PerkinElmer, Villebon-Sur-Yvette, France). Results were normalized to total protein content and expressed as count per minute (CPM)/ µg of proteins.
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 min at 37℃ and hybridized overnight at 42℃ with probe that was labeled with γ-32P-ATP (Perkin Elmer Cat # NEG035C; 6000 Ci/mmol). Expose as needed after washing with 2X SSC and 0.5% SDS for 3 times.