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Citations for PerkinElmer :
151 - 200 of 245 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA probes were prepared by end-point PCR and labeled with dCTP [α-32P] (PerkinElmer) using the Cytvia Amersham Megaprimer labeling kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Tris buffer containing trace amount of 32P-labeled orthophosphoric acid (∼1.2 pmol and ∼1 µCi, PerkinElmer) and different concentrations of cold phosphate (NaH2PO4 ...
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bioRxiv - Genetics 2020Quote: ... DNA methylation was measured by the incorporation of tritiated methyl groups from radioactively labeled SAM (Perkin Elmer) into the biotinylated substrate using an avidin–biotin methylation plate assay47 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then placed in a tracer buffer containing 1-125-labeled OVTA ligand (Perkin Elmer NEX2540) for 60 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were UV cross-linked and hybridized with miRNA probes labeled with [γ-32P] ATP (PerkinElmer), with U6 served as loading control ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were incubated for 5 min before addition of 200μCi 35S-labeled methionine/cysteine mixture (PerkinElmer) and incubation for 1 hr at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 0.5 uCi of 56 mCi/mmol [14C] acetic acid (Perkin Elmer Cat# NEC084H001MC) per well overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... the samples were immunostained as described above after detection of the Emi2 or cyclin B1 RNA probe with tyramide-FITC (PerkinElmer, Inc.) in in situ hybridization analysis ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were grown until OD600 ∼ 0.02 and labeled with 50 μCi/ml 32P-orthophosphate (900 mCi/mmol; PerkinElmer). When OD600 reached 0.2-0.4 ...
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bioRxiv - Microbiology 2020Quote: ... was used for hybridization with oligonucleotides 5’-end labeled with 6,000 Ci/mmol γ-32P ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled using an 35S Met/Cys labeling mixture at 200 µCi/ml (EasyTag Express35S; Perkin Elmer) for 30 minutes in media that contained drug treatments and that was 20 percent MEF culture medium as above and 80 percent DMEM lacking Met/Cys and with dialyzed FBS ...
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bioRxiv - Biochemistry 2023Quote: ... the beads were suspended in 25 µl of cytoplasmic buffer containing 2 µCi of 3H-labeled SAM (PerkinElmer) and incubated overnight at 32 °C without shaking ...
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bioRxiv - Immunology 2024Quote: ... and the fraction of displaced labeled peptide was quantified with a gamma counter (Perkin Elmer, Wallac 1272 CliniGamma). Binding curves were determined by plotting the amount of displaced labeled peptide vs the concentration of unlabeled competitor ...
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bioRxiv - Microbiology 2020Quote: ... FISH detection was performed using anti-biotin antibody (SA-HRP) and a FITC/Cy5 coupled secondary antibody with the TSA Plus system (Perkin Elmer, USA). Coverslips were stained with Hoechst ...
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with canine rough microsomes (tRNA probes, College Station, TX) and EasyTag 35 S-labeled methionine (PerkinElmer, Waltham, MA) for one hour at 30 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were labeled with 250-1000 µCi/ml of [35S]-Express label (1100 Ci/mmol; PerkinElmer, Boston, MA) at 37°C depending on the length of the pulse ...
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bioRxiv - Immunology 2021Quote: ... The 3H-labeled products were isolated using DEAE-Sephadex A-50 columns and quantitated by liquid scintillation counting (PerkinElmer). Non-isotopic methylated RNA fragments were prepared using cold SAM (Biolabs ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified RNA oligos were either phosphorylated with unlabeled ATP or 5’ end-labeled using [γ-32P] ATP (Perkin Elmer) and T4-Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... after 20 min proteins newly synthetized in the mitochondrial compartment were labeled with 100 μCi [35S]-methionine (Perkin Elmer) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and area of Map2-labeled somatodendritic region of neurons was quantified using Harmony® high-content analysis software (PerkinElmer). For Tau aggregation analysis ...
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bioRxiv - Microbiology 2022Quote: ... blood plasma collected from the tip of the tail vein was diluted with equal volume of PBS (pH 7.4) and analyzed for DX-4000-FITC concentration with a fluorescence spectrophotometer (HTS-7000 Plus-plate-reader; Perkin Elmer, Wellesley, Massachusetts, USA) at an excitation wavelength of 485 nm and emission wavelength of 535 nm ...
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bioRxiv - Immunology 2024Quote: ... After an additional 2 hours blood was collected and serum FITC-dextran levels were measured with a Victor V multiparameter plate reader (Perkin Elmer, Waltham, MA). Quantification of serum FITC-dextran levels was performed with the WorkOut 2.5 software by extrapolation against know concentrations of FITC-dextran standard.
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bioRxiv - Cell Biology 2020Quote: ... and labeled for 5 min with with 1 μL per OD of cells of EXPRESS 35S Protein Labeling Mix (PerkinElmer) for 5 or 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then treated with a mixture of [3H]-labeled steroids (final concentration, 10 nM T and 10 nM progesterone; ~1,000,000 cpm/well; PerkinElmer, Waltham, MA) at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pulse-labeled for the indicated times with 143 µCi/dish EasytagTM 35S Express Protein Labeling Mix (Perkin Elmer). After the pulse ...
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bioRxiv - Developmental Biology 2022Quote: ... A series of incubations was then performed to amplify the signal of the hybridized probe and label target probes for the assigned fluorescence detection channel (target probe was labeled for the assigned fluorescence detection channels Opal 520, Opal 570, Opal 650 and Opal 780, PerkinElmer). Nuclei were stained using a DAPI nuclear stain (RNAscope ref 323108 ...
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Neuroscience 2021Quote: ... The signal of CB1 receptor hybridization was revealed by a TSA reaction using cyanine 3 (Cy3)-labeled tyramide (1:100 for 10 minutes, NEL744001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Molecular Biology 2022Quote: ... Radioactively labeled tRNAs were produced by T7 in vitro transcription in the presence of [α-32P]-UTP (PerkinElmer, 10mCi/ml) using PCR products containing an upstream T7 promotor (see DNA constructs) ...
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bioRxiv - Cell Biology 2023Quote: ... The biodistribution of the fluorescently labeled nanoalgosomes was determined by in vivo near-infrared fluorescence imaging using an IVIS Spectrum scanner (IVIS Spectrum CT; PerkinElmer). Athymic nude mice (n=6 ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were labeled for 15 min at 37 °C with 100 µCi of 35S-methionine (PerkinElmer Life Sciences, Boston, MA). After incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µL of RNA was 3’-end-labeled with 32pCp (prepared by incubating 16.5 µl of γ-32P-ATP (PerkinElmer), 1 µl of T4 polynucleotide Kinase ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Cell Biology 2024Quote: ... using a HyBEZ® oven Probes were detected using Tyramide signal amplification (TSA) reaction (1:600 dilution) using an Alexa-flour labeled TSA kit (PerkinElmer) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... LPS-stimulated plasmablasts were starved for methionine and cysteine for 60 minutes and pulse labeled for 15 minutes with 55 µCi/1×106 cells of Express 35S protein labeling mix (Perkin Elmer). Samples were then diluted 10x with cell culture media containing 5mM unlabeled methionine and cysteine ...
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bioRxiv - Molecular Biology 2020Quote: ... The 100-bp mini-circle was prepared as follows: oligos cb1027 and cb1028 were labeled with [γ-32P]-ATP (Perkin Elmer) by T4 polynucleotide kinase ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes (listed in Supplementary Table S2) were 5′ 32P-end labeled with 0.3 mCi of γ-32P ATP (Perkin Elmer) by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs ...