Labshake search
Citations for PerkinElmer :
151 - 190 of 190 citations for Recombinant human IgE kappa L His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05□μl of 5□mM stock compounds in DMSO were dispensed into assay microplates with a Janus 384 MDT NanoHead (PerkinElmer). Final concentration of the screening compounds in the assay was 16LμM ...
-
bioRxiv - Microbiology 2023Quote: Brine samples BP3 and BP4 were spiked with L-[4,5-3H] leucine (Perkin Elmer, specific activity 100 Ci mmol-1) at a final concentration of 20 nmol leucine L-1 (Kirchman et al. ...
-
bioRxiv - Immunology 2024Quote: ... [3H]-thymidine was added at 0.5μl (∼0.5 μCi) per well and plates incubated for 6 h before cells were harvested to UniFilter plates (Perkin Elmer) using a Tomtec 96 Harvester ...
-
bioRxiv - Microbiology 2024Quote: ... Of each sample three replicates and two formaldehyde-fixed (final conc. 1.85 %) adsorption blanks were spiked with [methyl-3H]-thymidine and L-[14C(U)]-leucine (Perkin Elmer) at saturating concentrations of 20 nM and 150 nM ...
-
bioRxiv - Cell Biology 2019Quote: ... 3 OD600 units of early log phase cells were labeled with 80 mCi of L-[35S]-methionine/cysteine mix (Perkin Elmer). Immunoprecipitated proteins were separated on SDS-PAGE and exposed to phosphor screens ...
-
bioRxiv - Neuroscience 2019Quote: ... 100µl from each of the wells was transferred to a vial and scintillation fluid (4ml) added (Optiphase Hisafe 2, PerkinElmer, UK). Radioactivity was measured using a Packard Tri-Carb 2900TR liquid scintillation counter (PerkinElmer ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 μl of the supernatant was spotted in a scintillation tube and upon the addition of Ultima Gold scintillation liquid (Perkin Elmer) the signal was measured by scintillation counter.
-
bioRxiv - Cell Biology 2021Quote: ... 90 μL of the cell suspension was also plated in duplicate into wells of a 96-well black-walled plate (PerkinElmer OptiPlate), and Venus fluorescence was determined using an excitation wavelength of 485 nm (5 nm bandwidth ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1mM PMSF, 100mM KCl and 1 mCi of [3H]AdoMet (S-adenosyl-L-[methyl-3H] methionine, 80 Ci/mmol; SAM) (Perkin Elmer) or 0.15mM unlabeled S-adenosyl methionine for 45 min at 30°C ...
-
bioRxiv - Molecular Biology 2020Quote: Purified protein complexes were incubated at room temperature with a histone substrate at a final concentration of 500nM and approximately 500nM radioactive SAM (Adenosyl-L-methionine S-methyl3H from PerkinElmer (NET155H250UC)) in methyltransferase buffer (10% glycerol ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated 10 minutes at 37 °C by addition of 1 μCi/ml of L-[3,4-3H]-Glutamic acid (Perkin Elmer, NET490) and 10µM L-glutamate (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: ... rabbit reticulocyte lysate was incubated with 1μg of the appropriate expression plasmid and 2mL of [35S] L-methionine (1,000 Ci/mMol at 10mCi/mL) (Perkin Elmer, NEG709A) for 90 minutes at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Analytical estimation of the stock CoCl2 solution concentration (100 mmol L−1) was performed employing inductively coupled mass spectrometry (Perkin Elmer Avio 200 optical emission spectrometer ...
-
bioRxiv - Microbiology 2024Quote: ... and the uptake of 14C(U) l-arginine was measured in the bacterial lysates using a liquid scintillation counter (Perkin Elmer).
-
bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
-
bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
-
bioRxiv - Cancer Biology 2020Quote: ... proteins were first labeled for 48 h with 0.25 μM Ci ml-1 L-[U-14C] VALINE (Perkin Elmer, Waltham, MA, USA) in GIBCO-RPMI 1640 medium (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... were measured using a radiometric assay that detects transfer of a [3H] methyl from the radio-labeled cofactor S-[Methyl-3H]-adenosyl-L-methionine (SAM, PerkinElmer, cat. NET155001MC) to a biotinylated peptide Akt1-K64 (QCQLMKTERPRPNTFIIRCLQW ...
-
bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
-
bioRxiv - Molecular Biology 2023Quote: DNA methylation of replicating sperm or erythrocyte nuclei in egg extract was assayed by the incorporation of 3H-SAM (S-[methyl-3H]-adenosyl-L-methionine; Perkin Elmer, NET155H). Demembranated sperm nuclei were prepared as published previously (86) ...
-
bioRxiv - Genomics 2019Quote: ... FISH experiments were performed using human fosmids containing non-duplicated region directly labeled by nick-translation with Cy3-dUTP (PerkinElmer), Cy5-dUTP (PerkinElmer) ...
-
bioRxiv - Genomics 2021Quote: Two-color FISH experiments were performed using human fosmid (n=2) or BAC (n=2) clones directly labelled by nick-translation with Cy3-dUTP (PerkinElmer) and fluorescein-dUTP (Enzo ...
-
bioRxiv - Immunology 2020Quote: ... during the final 6 hours (murine MLR) or 15 hours (human MLR) of culture was measured by a TopCount (PerkinElmer).
-
bioRxiv - Bioengineering 2023Quote: ... overexpressing the human FGFR1c together with β-klotho was plated into 384-well micro-titer plates (Perkin Elmer, Cat. #6007480) and incubated at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... sections were washed in TBST and incubated with Alexa Fluor® 594 AffiniPure Goat Anti-Rabbit IgG (H+L) (Abacus DX, Australia) with Spectral DAPI (FP1490) added (1/1000) (Perkin Elmer, USA) for one hour at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: NPCs were seeded onto poly-L-ornithine/laminin-coated 6-well and cyclic olefin co-polymer-based 96-well Cell Carrier Ultra microtiter plates (Perkin Elmer, Waltham, MA) at 1×105 and 2×103 cells/well ...
-
bioRxiv - Bioengineering 2022Quote: Quantification of the metals present in the shell using ICP-OES was performed in the year 2016 with freshwater L fortunei shell only with an inductively coupled atomic emission spectrometer (ICP OES, Perkin Elmer-Optima 3000). In 2022 we again quantified the metals present in shell samples of the L fortunei and in shell samples of marine P perna mussel ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were then mechanically detached using 275 μL DPBS and 80 μL of the resuspension was distributed in triplicate to a 96-well black/white isoplate (Perkin Elmer Life Sciences). Next ...
-
bioRxiv - Biochemistry 2024Quote: ... Ninety μl of cells pre-incubated with D-luciferin were added to a white opaque 96-well plate (Opti-Plate, PerkinElmer Life Sciences, 6005290) before measuring luminescence without filters at 28 °C every 10 s in a BMG Labtech POLARStar Omega plate reader ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... tumors (ca 120,000 cells) consisting of H460 human NSCLC transfected with luciferase and the tdTomato gene (H460 Luc+ tdTomato+, Perkin Elmer France) were injected ectopically into the left flank of 90 mice ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
-
bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
-
bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
-
bioRxiv - Developmental Biology 2020Quote: Recombinant GST-SRF protein or synthetic SRF peptides were incubated for 3 h at 30 °C with GST-SET7 in the presence of 100 nCi of S-adenosyl-[methyl-14C]-L-methionine [14C-SAM] (Perkin Elmer, Waltham, MA, USA) in HMTase assay buffer (50 mM Tris–HCl [pH 8.5] ...
-
bioRxiv - Microbiology 2022Quote: ... and 200 μl of parasites were applied to each well of HFF cells grown in 96-well plates with transparent bottoms (Perkin Elmer cat. no. 6055300). Plates were centrifuged at 290 g for 5 minutes ...
-
bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
-
bioRxiv - Immunology 2024Quote: ... Five-micrometer serial sections were stained with a human-specific anti-COXIV antibody and scanned using the Vectra® Polaris Automated Imaging System (PerkinElmer, USA) at 20×magnification ...
-
bioRxiv - Cell Biology 2021Quote: ... hand-picked human islets were dispersed in accutase for 10 min at 37°C and plated in 96-well plates (Perkin Elmer Inc., Waltham, MA) treated with Poly-D-Lysine Hydrobromide (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...