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Citations for PerkinElmer :
1 - 50 of 190 citations for Recombinant human IgE kappa L His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Neuroscience 2022Quote: ... and kappa (#ES-541-C) opioid-expressing CHO cell lines from PerkinElmer (Waltham, MA) were used ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Bioengineering 2024Quote: ... Vivo-Tag 680 XL (PerkinElmer #NEV11119) was used as a fluorescent dye to track idLFA1 and control murine serum albumin (MSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Cell Biology 2019Quote: ... Easy-Tag [35S]-Cys/Met was purchased from PerkinElmer Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then washed twice with PBS and incubated with either H-H or L-L medium supplemented with 1μCi per well of L-[U-14C]-Aspartic acid (PerkinElmer, NEC268E050UC) for 40 h ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... and 100μl Easy Tag Express 35S Methionine/Cysteine (Perkin Elmer NEG 772002MC) at either 0 or 24h after transformation with a 6xMyc-Pex14 (pAMW-Pex14 ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Physiology 2022Quote: ... Radiolabeled L-[3,4-3H]-Arginine monohydrochloride or glutamine-L-[3,4-3H(N)] (Perkin Elmer) was added to a final concentration of 8 and 2 µM respectively (2 and 0.5 μCi in a total volume of 50 µl) ...
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bioRxiv - Cancer Biology 2022Quote: ... (200 mg/L, PerkinElmer #122799) and bioluminescence was acquired 10 minutes after the luciferin injection in an IVIS Spectrum ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Biophysics 2020Quote: ... or L-[35S]-Met (Perkin Elmer). For each sample ...
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bioRxiv - Immunology 2020Quote: ... EasyTag L-[35S]-Methionine (Perkin Elmer) was added to the TNT Quick Coupled Transcription/Translation reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... L-[2,3-3H]-Alanine (PerkinElmer NET348250UC), L-[1,2-14C]-Alanine (PerkinElmer NEC266E050UC) ...
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bioRxiv - Developmental Biology 2022Quote: ... L-[3H(G)]-Serine (PerkinElmer NET248250UC), L-[14C(U)]-Glycine (PerkinElmer NEC276E050UC) ...
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bioRxiv - Developmental Biology 2022Quote: ... L-[3,4-3H]-Glutamine (PerkinElmer NET551250UC), L-[2,3-3H]-Alanine (PerkinElmer NET348250UC) ...
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bioRxiv - Developmental Biology 2022Quote: ... L-[14C(U)]-Glycine (PerkinElmer NEC276E050UC), or L-[3,4-3H]-Glutamate (PerkinElmer NET490001MC ...
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bioRxiv - Developmental Biology 2022Quote: ... L-[1,2-14C]-Alanine (PerkinElmer NEC266E050UC), L-[3H(G)]-Serine (PerkinElmer NET248250UC) ...
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bioRxiv - Biophysics 2023Quote: ... 1 μM 3H-L-Asp (PerkinElmer), and 0.5 μM valinomycin ...
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bioRxiv - Developmental Biology 2022Quote: ... or L-[3,4-3H]-Glutamate (PerkinElmer NET490001MC) for 5 minutes at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... L- [35S]-methionine was provided by PerkinElmer.
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bioRxiv - Biochemistry 2021Quote: ... L-[3H]tyrosine (10 Ci/mol; PerkinElmer) and L-[3H]alanine (5 Ci/mol ...
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bioRxiv - Biochemistry 2022Quote: ... L-[35S]-methionine was provided by PerkinElmer. Anti-HA tag antibody (mouse monoclonal ...
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bioRxiv - Immunology 2022Quote: ... and L-[35S]cysteine (PerkinElmer Life Sciences), protein molecular weight markers ...
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bioRxiv - Molecular Biology 2021Quote: ... L-[35S]-methionine was obtained from PerkinElmer. Mouse monoclonal antibody against the HA antigen was purchased from BioLegend ...
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bioRxiv - Molecular Biology 2022Quote: ... L-[35S]-methionine was obtained from PerkinElmer. Mouse monoclonal antibody against the HA antigen was purchased from BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 µM 3H-L-Asp (PerkinElmer). For negative controls without Na+-gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then incubated at 37°C for 1 minute in 1 mL of Na+-free HBSS containing 1 μmol/L of L-[14C]-leucine (0.03 μCie/mL; PerkinElmer). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated for 5 minutes at 37°C with 55µCi/well of 35S-L-methionine and 35S-L-cysteine Promix (Perkin Elmer) in a minimal volume of culture medium ...
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bioRxiv - Molecular Biology 2019Quote: Cells were incubated with complete growth media supplementing with EasyTagTM Express Protein Labeling Mix containing both [35S]-L-methionine and [35S]-L-cysteine (PerkinElmer) at 10 μCi/ml for 30 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: Influx and efflux assays were initiated after mixing 20 μl of PLs solution with 180 μl of influx transport buffer (10 μM L-serine in Tris buffer with and 0.5 µCi of L-[3H]-serine (Perkin Elmer) or 4 mM cold amino acid for efflux experiments ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... L-[3,43H(N)]-glutamine (3H-glutamine; PerkinElmer, #NET551001MC) uptake was carried out using 1 million cells resuspended in 0.4 mL HBSS (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...