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Citations for PerkinElmer :
151 - 200 of 1166 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated in binding buffer with a constant concentration of 125I-GIP (30 pM, PerkinElmer) and increasing concentrations of unlabeled tirzepatide or peptide 20 (3.57 pM to 1 μM ...
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bioRxiv - Biophysics 2024Quote: ... All measurements were performed in black half-area 96-well format low-binding OptiPlates (#6052260, PerkinElmer) for fluorescence and half-area UV-Star microplates (#675801 ...
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bioRxiv - Neuroscience 2019Quote: ... the hybridization signal was amplified with a TSA Plus Biotin Kit (Perkin Elmer, Waltham, MA), by diluting the TSA Plus biotin reagent (1:400 ...
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bioRxiv - Neuroscience 2022Quote: ... Signals were revealed with the Tyramide Signal Amplification (TSA plus biotin) kit (PerkinElmer, cat. #NEL749A001KT) at 1:100 concentration ...
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bioRxiv - Microbiology 2020Quote: ... the mixtures were spotted onto filtermat B (Perkin Elmer, cat#1450-521) soaked in 0.5% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The reactions were terminated through filtration with GF/B filters (Perkin Elmer) using a 96-well plate harvester (Brandel) ...
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bioRxiv - Neuroscience 2022Quote: ... Binding was performed with the selective MOR agonist [3H]DAMGO (53.7 Ci/mmole, Perkin Elmer, Boston, MA) in 50 mM Tris-HCl buffer (pH 7.4) ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with Biotinyl Tyramide Amplification kits (TSA, TSA plus biotin kit, PerkinElmer), following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... monomer equivalents of fibrils (α-synuclein or ASC) were coated onto high-binding 384-well SpectraPlates (Perkin Elmer) as serial dilutions (13-fold 1:2 serial dilution) ...
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bioRxiv - Biochemistry 2024Quote: Fluorescence was measured using the Luminescence Spectrometer L 50 B from Perkin Elmer. Spectra were recorded with an excitation wavelength of 280 nm using a 5 x 10 mm cell at 25 °C with excitation and emission slits of 3 and 5 nm ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP-labeled goat anti-rabbit antibody (1:500, PerkinElmer, NEF812001EA) for secondary detection ...
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bioRxiv - Molecular Biology 2020Quote: ... scintillation cocktail and binding quantified using scintillation counting for H3 on an Tri-Carb 2810TR scintillation counter (Perkin Elmer).
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bioRxiv - Neuroscience 2020Quote: ... Probe binding was indicated by deposition of target-specific fluorophores at each location via TSA Plus Fluorescence kit (PerkinElmer), and sections were then counterstained with DAPI ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT, 0.5 % Sarkosyl, 0.4 u/μl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Equal amounts of nuclei extracted from Drosophila S2 cells were used as spike-in material in run-on reactions ...
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bioRxiv - Zoology 2022Quote: ... The horseradish peroxidase-conjugated Avidin-Biotin Complex was visualized using TSA Plus Fluorescein System (PerkinElmer, Waltham, MA, USA) or 3,3-diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mM DTT, 0.5% Sarkosyl, 0.4 U/µl RNase inhibitor, 0.025 mM biotin-ATP/CTP/GTP/UTP [Perkin Elmer]). Total RNA was extracted using Trizol and ethanol precipitation ...
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The reaction mixture was then filtered over a GF-B filter plate (Perkin Elmer) followed by four quick washes with ice-cold 50 mM Tris HCl ...
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bioRxiv - Biophysics 2023Quote: ... Filter plates were dried at 96℃ before scintillation sheet (MeltiLex B/HS, Perkin Elmer) was melted on to the filter ...
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bioRxiv - Cell Biology 2023Quote: ... or ECL anti-rabbit antibodies (NA934V; 17434242) (Amersham) were used for detection of probed proteins using enhanced chemiluminescence (ECL)(Perkin Elmer). Chemiluminescence was detected using an iBright FL1500 (ThermoFisherScientific ...
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... 100 nM GBR12909 (to block binding to dopamine transporter) and 50 pM [125I]RTI-55 (NEX272, Perkin Elmer, Boston, MA) were added to the buffer ...
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bioRxiv - Biophysics 2023Quote: ... Leucine affinities for WT LeuT and mutants displaying WT substrate affinities (A22V, A22S) were determined by saturation binding of [3H]leucine (25 Ci/mmol) (PerkinElmer), with unspecific binding corrected for by the addition of 100 µM unlabelled leucine ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Plates were then filtered on GF/B filter plates precoated with 0.1% PEI (PerkinElmer #6005177) and washed with 1.2mL wash buffer and fan dried ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP) conjugated secondary antibody was added in order for labelled proteins to be detected by Western Lightning Plus-ECL (PerkinElmer, 203-170071). Primary and secondary antibodies used are listed in Supplementary Table 5.
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Biochemistry 2019Quote: ... The BMAL TAD probe was diluted to a working concentration of 50 nM in assay buffer and binding was monitored by changes in fluorescence polarization with an EnVision 2103 multilabel plate reader (Perkin Elmer) with excitation at 531 nm and emission at 595 nm ...
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bioRxiv - Biochemistry 2023Quote: MLL1C binding assays (Figure 2C) were performed as above except using Nickel-chelate acceptor beads (10 μg/mL; Perkin Elmer AL108M), streptavidin donor beads (20 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... which were incubated with various concentration of synthetic biotinylated TAR-RNA (biotin 5’-dT/GGU CUC UCU GGU UAG ACC AGA UCU GAG CCU GGG AGC UCU CUG GCU AAC UAG GGA ACC/3’-biotin dT) (Integrated DNA Technologies) at 4°C for 3 h in the AlphaLISA buffer (PerkinElmer). Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer ...
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bioRxiv - Genomics 2021Quote: ... Aliquots of 10E6 nuclei were subjected to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) and PRO-seq libraries were constructed in duplicate as described (31) ...
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bioRxiv - Genomics 2022Quote: ... PRO-seq libraries for all replicates were then prepared simultaneously by subjecting one aliquot of 1e7 nuclei/sample to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) following our previously detailed protocol (24) ...
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bioRxiv - Genomics 2022Quote: ... nuclei were extracted from ~2×107 cells and run-on reactions were carried on at 37°C for 3 minutes using 3μl of each 11-biotin-labelled ribonucleotide stocks (Perkin Elmer). Following real time PCR amplification in the presence of EvaGreen dye as above ...
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bioRxiv - Genomics 2023Quote: The nuclear run-on experiments for PRO-seq were performed in triplicate as described in 47 with the use of a mixture of rNTPs and Biotin-11-CTP (PerkinElmer), and a range of 10-20 million isolated nuclei per sample ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were 1:10,000 of HRP-labeled α-mouse (for Flag) (PerkinElmer) or α-rabbit (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Daily calibration and normalization of the PET scanner were performed using a 68Ge/68Ga phantom and cross-calibration factor was determined with a γ-counter (Wizard2; PerkinElmer). Synthesis and formulation of the radiotracer were done as described in (Holschbach et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Microtiter 384-well Proxiplates for the ThermoFRET stability assay and 384-well Optiplates for association binding experiments were obtained from PerkinElmer (Beaconsfield, UK).
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bioRxiv - Neuroscience 2024Quote: ... and 50 µl of radioligand diluted in binding buffer ([3H]-N-methylspiperone: 0.4 nM final concentration, Perkin Elmer, Waltham, MA; [3H]-(R)-(+)-7-OH-DPAT ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... The saturation binding assay was conducted using an 8-point dose-dependent concentration of [3H] ketanserin (maximal concentration 20 nM) (Perkin Elmer, Waltham, MA) with brain crude membranes (25 μg membrane proteins ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). Compounds were then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μL of protein mixture was added to each well of a 1/2 area 96-well OptiPlate (PerkinElmer). FKBP12F36V competitor or p300-BRD competitor was then added from DMSO stock to protein mixture (0.4 μl/well ...
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated overnight at 4ºC with horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:1000 and 1:500 for detection in PMm/PMg and other nuclei, respectively; PerkinElmer, Waltham, MA) and Dylight 549-conjugated goat anti-rabbit IgG (diluted 1:500 ...