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Citations for PerkinElmer :
451 - 500 of 1166 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The samples were incubated for 30 min at 37 °C in the presence of the EasyTag Express Protein Labeling Mix 35S (PerkinElmer), pelleted ...
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bioRxiv - Synthetic Biology 2019Quote: ... Radioactive counts from TCA-precipitated samples was measured by liquid scintillation to then quantify soluble and total yields of each protein produced as previously reported (MicroBeta2; PerkinElmer).39,40
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bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
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bioRxiv - Synthetic Biology 2020Quote: ... Radioactive counts from TCA-precipitated samples was measured by liquid scintillation to then quantify soluble and total yields of each protein produced as previously reported (MicroBeta2; PerkinElmer).27,30
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bioRxiv - Immunology 2021Quote: ... The excitation and emission wavelengths for each protein were determined prior to the stopped-flow experiments using an LS55 luminescence spectrophotometer (PerkinElmer). The oxidant concentration range required to lead to fluorescence changes for each protein was also determined.
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bioRxiv - Cell Biology 2019Quote: ... loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit; PerkinElmer). For Flag immunoprecipitations ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then separated with SDS-PAGE and detected by phosphor imaging (Cyclone Plus storage phosphor system, Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... 15 or 10-20% SDS-PAGE gels and proteins were transferred onto PVDF membranes (0.45 μm, Perkin Elmer, Waltham, MA) and blocked with 5% BSA/TBS-T for 2 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured in 6-well plates and metabolically labeled (Easytag Express 35S-Met-Cys protein labeling mix (Perkin Elmer)) with 0.2 mCi/ml for various times ...
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bioRxiv - Bioengineering 2023Quote: ... of approximately 5.7 ± 3.0 pmol/mg protein measured by the manufacturer using [3H]-DAMGO (ES-542-C OP3 ValiScreen, PerkinElmer, Waltham, MA) were obtained and cultured for a series of binding assays ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Cell Biology 2023Quote: ... BMK-dko cells stably expressing a mCerulean3-fused anti-apoptotic protein were seeded at 4000 cells/well into a CellCarrier-384 Ultra Microplate (PerkinElmer). 24 hours later ...
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bioRxiv - Biophysics 2023Quote: ... The absorption and emission spectra of the purified protein were recorded at RT (Lambda365, Perkin Elmer and FLS980, Edinburgh Instruments)
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bioRxiv - Biochemistry 2023Quote: ... Protein biosynthesis rates were determined by measuring the incorporation of radioactive methionine in auranofin-treated and untreated samples after protein precipitation with 20 % (w/v) trichloro acetic acid using a scintillation counter (Tri-Carb 2800TR, PerkinElmer). 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm ...
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bioRxiv - Genetics 2023Quote: ... and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer). Cells were chased in RPMI supplemented with 0.4 mM methionine for 0 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were resolved by 12% SDS-PAGE and dried gels were exposed to a Cyclone Plus Storage Phosphor Screen (PerkinElmer). The screen was scanned using a Typhoon TRIO Variable Mode Imager (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... 100 µL of cell homogenate (0.05 mg protein/well) and 100 µL of [3H]8-OH-DPAT (0.5 nM final concentration, 170 Ci/mmol, Perkin Elmer) in a final volume of 1 mL ...
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bioRxiv - Biochemistry 2024Quote: The excitation and emission spectra of the pure proteins diluted in PBS were recorded on an LS-55 fluorescence spectrometer from PerkinElmer. The emission spectrum of each protein was obtained by fixing the excitation wavelength at its maximum absorbance wavelength ...
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bioRxiv - Physiology 2020Quote: ... TSA® Plus fluorophore for channel 1 (fluorescein, PerkinElmer; 1:1000; 30 min), HRP blocker (15 min) ...
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bioRxiv - Biophysics 2019Quote: ... USA and phosphatidylcholine (DPPC, L-a-dipalmitoyl [1-palmitoyl-1-14C] from PerkinElmer, Inc ...
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bioRxiv - Neuroscience 2019Quote: ... The anti-fluorescein antibody was visualized by using the TSA Plus Fluorescein System (PerkinElmer) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... membranes were incubated secondary antibodies and developed using Western Lightning® Plus-ECL (PerkinElmer).
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bioRxiv - Cancer Biology 2021Quote: The intrinsic and extrinsic fluorescence emission spectra of the proteins were measured using an LS55 spectrofluorometer (Perkin Elmer, Waltham MA, USA). WT or N16D HsTPI (0.5 mg·mL-1 ...
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bioRxiv - Cell Biology 2020Quote: ... the PVDF membrane was washed with TBST and incubated with appropriate secondary antibodies (horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG), followed by enhanced chemiluminescence (ECL; Visual protein, Taipei, Taiwan) and the membrane was examined by ECL spectrophotometry (Perkin Elmer, Geliance 600 Camera Imaging system ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was transferred to a tube and the protein-bound [α-32P]dCTP was quantified in a Beckman Coulter LS 6500 Liquid Scintillation Counter using OptiPhase HiSafe 3 (PerkinElmer) as solvent.
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were pulsed (0.45MBq/106 cells) for 10 min with 35S Cys/Met (EasyTagTM Express Protein Labelling, Perkin Elmer, Beaconsfield, UK) in DMEM without Cys/Met ...
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bioRxiv - Microbiology 2020Quote: ... and adhesin were optimized by finding the hook point for the interaction by setting up a matrix of proteins in 96 well AlphaPlates (Perkin Elmer) in 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Biochemistry 2020Quote: ... with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol) from Perkin Elmer. Protein Assay (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved for cysteine and methionine for 15-30 min and pulse labeled for 5 min with 55 µCi/ 35-mm dish of Easytag express 35S protein labeling mix (Perkin Elmer). Where indicated (+DTT) ...
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bioRxiv - Microbiology 2021Quote: ... After these incubations the membranes were washed again with PBS-T and proteins were visualized by Western Lightning Chemiluminescence Reagent Plus (Perkin Elmer).
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bioRxiv - Immunology 2021Quote: ... The radioactive counts (cpms) of immunoprecipitated protein was quantified using a 96-well Microbeta Trilux liquid scintillation plate reader (Perkin Elmer). Antibody index for each sample was calculated as follows ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pulse labeled for 15 minutes with 132 μCi/6 cm dish with EasyTag Express 35S Protein Labeling Mix (Perkin Elmer). Radiolabeling was stopped by adding excess ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The recruitment of β-arrestin2 by MOR agonists after G protein activation was assessed using the Cisbio HTRF-based β-arrestin2 recruitment assays (Cisbio Assays, PerkinElmer), which monitored the interaction between β-arrestin2 and AP2 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; PerkinElmer).
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bioRxiv - Developmental Biology 2021Quote: ... The reaction with tyramide-Cy3 (1:50 dilution in 1 ×Plus Amplification Diluent (PerkinElmer), followed by 1:100 dilution with DW ...
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bioRxiv - Genomics 2020Quote: ... Prox-1 probes were visualized by Opal 650 reagents (1:1500 dilution, Perkin Elmer). Slides were additionally co-stained with rabbit anti-human vWF polyclonal antibody (Dako ...
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bioRxiv - Immunology 2021Quote: ... 1 μCi α32P-ATP (PerkinElmer)) for 90 min at 37°C ...
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bioRxiv - Immunology 2019Quote: ... 1 s per well (PerkinElmer). The ID50 was calculated as the dilution that resulted in a 50% reduction in relative luminescence units (RLU ...
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bioRxiv - Developmental Biology 2021Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Immunology 2021Quote: ... The NIT-1 cells were then labeled with 1 μCi/well of 51Cr (Perkin Elmer) for 3 hours at 37°C and co-cultured with AI4 T cells at a E:T ratio of 20:1 for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μCi of 2-Deoxy-D-[1-2-3H] glucose (PerkinElmer, Boston, MA, USA # NET328A001MC) and 10μM non-radioactive 2-DG (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted 1/5 or 1/10 in 1X Immunoassay buffer (Perkin Elmer, #AL000F). A 2-fold serial dilution of imiglucerase (Genzyme ...