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Citations for PerkinElmer :
1051 - 1100 of 1530 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... After an additional 2 hours blood was collected and serum FITC-dextran levels were measured with a Victor V multiparameter plate reader (Perkin Elmer, Waltham, MA). Quantification of serum FITC-dextran levels was performed with the WorkOut 2.5 software by extrapolation against know concentrations of FITC-dextran standard.
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... the same cell samples were further lysed and digested with 2% HNO3 for intracellular iron content quantification using an ICP-AES instrument (Perkin Elmer Optima 8000).
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bioRxiv - Plant Biology 2024Quote: ... five seedlings were grouped into one scintillation vial containing 2 ml Ultima Gold liquid scintillation cocktail and counted in a Tri-Carb 4910TR Liquid Scintillation counter (Perkin Elmer, Rodgau, Germany).
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... with HRP goat anti-mouse IgG Ab (31430, 1:20,000) for 1 h and developed using a Western Lightening Enhanced Chemiluminescence kit (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... 20 μl of a 1:1 mix of Western Lightning oxidizing and enhanced luminol reagents (Perkin Elmer Life Sciences) was added to each well ...
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed by exposition to a (1:1) solution of Western Lightning® Plus-ECL reagents (Perkin Elmer) for 1 minute and chemiluminescence imaging in a ChemiDoc™ Imaging System (BioRad).
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bioRxiv - Microbiology 2024Quote: ... seven or eight replicate bottles were filled and spiked with 50 µCi [14C]-bicarbonate (specific activity 56 mCi mmol−1 / 2.072 × 109 Bq mmol−1; PerkinElmer). To three bottles ...
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bioRxiv - Microbiology 2021Quote: ... BIOTECHNOLOGIES Ltd) before counting on a HINDEX 300SL in 5 ml of scintillation fluid (Scintillation fluid, ULTIMA GOLD™, Perkin Elmer, Inc. LOT No.: 77-18151).
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bioRxiv - Genetics 2023Quote: ... A sample of cells were stained with 10 µg ml–1 Hoechst plus 1:500 acridine orange/propidium iodide solution and counted on an Opera Phenix confocal microscope (PerkinElmer). A total of 100,000 cells per well were seeded into neural coating medium-filled 96-well plates in 100 µl per well of neural medium comprising Brainphys medium with 1× B27 plus vitamin A ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was washed three times and Fixer-Developer (1:1) solution from Western Lightning Chemiluminescence Reagent Plus (PerkinElmer, NEL104001EA) enhanced chemiluminescence (ECL ...
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bioRxiv - Plant Biology 2020Quote: ... 5 to 10 mg powder were mixed with 2 ml scintillation cocktail and counts per minute (cpm) recorded with a TRI-Carb 2810TR liquid scintillation analyzer (Perkin Elmer, Waltham, MA, USA).
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated at room temperature for 2 h before measurement of the fluorescence using an EnVision Multimode Plate (Reader PerkinElmer Life and Analytical Sciences). All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100 μM forskolin in each cell line ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was then extracted from 2 ug of total RNA per sample using NEXTFLEX® Poly(A) Beads 2.0 (Perkin Elmer, Austin, Texas, USA). Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-DNP-HRP antibody (1:1000, Perkin Elmer) and tyramide signal amplification (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... A buffer containing Cy3 (PerkinElmer, Waltham, 1:50) was used to stain the samples for 40 min.
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bioRxiv - Microbiology 2021Quote: ... and 1 U of AmpliTaq polymerase (Perkin Elmer). The mixture was subjected to 40 cycles of amplification using a DNA thermal cycler 2720 (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated for 1 min ECL (Perkin Elmer, USA) and the signal was determined with an INTAS ECL Chemocam imager (INTAS Science Imaging ...
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bioRxiv - Biophysics 2022Quote: ... and 1:125 Streptavidin-XL665 (HTRF acceptor, PerkinElmer) in assay buffer (25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Developmental Biology 2022Quote: ... and anti-DNP-HRP (PerkinElmer FP1129; 1:100) were used in combination with DyLight488-tyramide and DyLight680-tyramide ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μM [3H] SAM (82.3 Ci/mmol, PerkinElmer) and various amounts of refolded RNA in 1X binding buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... and Opal 690 (1:1500, PerkinElmer, Cat. FP1497001KT).
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bioRxiv - Biophysics 2024Quote: ... 1 μL γ 32P ATP (Perkin Elmer, NEG502Z250UC), 1 μg PM187B20 in a 20 μL reaction volume of 1x T4 PNK buffer (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... were then added to the COS-7 cells in the presence of 50 μg/ml PI and imaged immediately at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 40X 1.3 N.A. oil objective). Images were acquired at 1 frame/20s and analyzed using NIH ImageJ software ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Microbiology 2022Quote: 643nt pre-crRNA was in vitro transcribed with [a-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) as described above ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were counterstained with DAPI (1 μg ml-1) before imaging with the Opera Phenix High Content Screening System (Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... the medium was removed from all wells and cells were lysed by the addition of 100 µl of a 1:1 dilution of BriteLite assay readout solution (Perkin Elmer) and 1xDPBS (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Assays were read out with luciferase substrates 2 d after infection by removing the media from the wells and adding 80 uL of a 1:1 dilution of BriteLite in DPBS (BriteLite Plus, Perkin Elmer). Luminescence values were measured using a microplate reader (BioTek Synergy™ HT or Tecan M200) ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml cultures were grown in the presence of [32P]-phosphate (final concentration of 1 µCi ml-1; Perkin Elmer NEX060001MC) for until OD600 reached ∼1.5-2 ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml cultures were grown in the presence of [1-14C]-acetate (final concentration of 0.2 µCi ml-1; Perkin Elmer NEC084A001MC) until OD600 reached ∼0.5-0.7 ...
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bioRxiv - Neuroscience 2024Quote: ... The probes were visualized using Opal 520 and Opal 570 reagent (1:500 and 1:1000, respectively; PerkinElmer, Woodbridge, ON, CA). PHOX2B immunoreactivity was detected using mouse monoclonal PHOX2B antibody (B-11 ...
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bioRxiv - Bioengineering 2024Quote: ... and scanned four times in the 4000 cm-1 to 650 cm-1 range using transmission mode in the FTIR software (PerkinElmer Spectrum). The peaks from the FTIR readings were analyzed via an infrared spectroscopy tables found in the literature [36].
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bioRxiv - Biochemistry 2024Quote: ... NADPH oxidation was monitored in 1-mL quartz cuvettes with a path length of 1 cm in a Lambda 650 UV/VIS spectrometer (Perkin Elmer) with attached water Peltier system set to the appropriate temperature ...
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... and SYBR Gold to the specified dye concentrations (0.03 to 60 μM) and analyzed the sample in a 10 × 2 mm2 cuvette (Perkin Elmer UV/VIS Spectroscopy Cell B0631122) for 5 min for each condition ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Animals were injected intravenously 24h after the last pharmacological treatment with Hypoxisense 680 (2 nmol/100 uL per mouse, Perkin Elmer, Life Sciences, Zaventem, Belgium), and the Hypoxisense 680 was measured in vivo 24h post-injection using the IVIS Spectrum (Perkin Elmer ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each mouse was injected retroorbitally with 80 μl of solution containing a 1:1 mixture of MMPSense 750 FAST Fluorescent Imaging Agent (MMP750) (PerkinElmer, Waltham, MA) and monoclonal anti-type II collagen antibody that had been coupled with 680 dye (MabCII680 ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB 942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a horseradish peroxidase-conjugated anti-fluorescein antibody (diluted 1:500 with DIG-1, PerkinElmer, Foster City, CA) for 1 h and washed twice with DIG-1 for 5 min each time ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Biophysics 2024Quote: ... and the released 14CO2 was trapped in 20 ml of scintillation cocktail (Carbo-Sorb E:Permafluor® E+, 1:1 [v/v], PerkinElmer, Groningen, Netherlands). The radioactivity of the rhizosheath was measured using a liquid scintillation counter (PerkinElmer® Tri-Carb 4910TR ...
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bioRxiv - Biochemistry 2024Quote: ... and for 15 min at 30°C (Gαi1) with 1 μM [γ-33P]-GTP (Hartmann Analytic GmbH) or 1 μM [γ-32P]-GTP (Perkin Elmer) in Reaction Buffer (50 mM HEPES ...
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bioRxiv - Developmental Biology 2024Quote: ... Transcripts were fluorescently labeled with Opal 520 (1:1500) and Opal 570 (1:500) using the Opal 7 Kit (Perkin Elmer; NEL797001KT). Images were acquired on a Zeiss LSM 880 inverted confocal system with 20X air and 40X oil objectives using Zen Black software (Carl Zeiss) ...