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Citations for PerkinElmer :
951 - 1000 of 1530 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA samples were separated on 2% glyoxal agarose gels and transferred onto nylon membranes (GeneScreen Plus, Perkin Elmer). Complementary [32P]-labeled probes were synthesized by linear PCR and hybridized at 65°C overnight in hybridization solution (0.5 M NaP pH = 7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1-2 μg of total RNA was used to isolate poly-A RNA with NEXTFLEX Poly(A) beads 2.0 (Perkin Elmer), while libraries were prepared using the NEXTFLEX Rapid Directional RNA-seq Kit 2.0 (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: ... The plate was scanned and images were acquired in real-time every 2 hours from 0 to 24 h post-treatment by Opera Phenix High Content Screening System by PerkinElmer, Inc ...
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bioRxiv - Microbiology 2024Quote: ... plates were incubated in the dark at 37°C + 5% CO2 before their fluorescent signal was measured at 2 hours post-incubation at 485/535nm using the Victor3V microplate reader (PerkinElmer). Background signal from unstimulated cells were subtracted from zymosan stimulated samples for analysis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3.7 MBq/mL) and [2-14C] acetic acid (2.183 GBq/mmol, 37 MBq/mL) were purchased from PerkinElmer (Waltham, MA). For the ultra[performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with Ham’s F-10 media supplemented with 1.5 mCi/well myo[2-3H(N)] inositol (Perkin Elmer/Revvity) for 16-18 h ...
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bioRxiv - Biochemistry 2024Quote: ... Radioactivity was measured (counts per minute, CPM) using a scintillation counter (MicroBeta 2 Plate Counter) with a scintillation cocktail (OptiPhase SuperMix, PerkinElmer). Data were analyzed via nonlinear regression in GraphPad Prism v10.
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 80-120 mg/kg body weight) / xylazine (Xy, 5-10 mg/kg body weight) cocktail) using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer, Rodgau, Germany) with images acquired using the Living Image® software package (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: ... The washed filters were dried for 30 min under a heat-lamp and placed in a 20-ml Borosilicate Glass Scintillation vial containing 5 ml of Ultima Gold F liquid scintillation cocktail (Perkin Elmer, MA, USA). 14C radioactivity retained on the filters was quantified using an LS6500 scintillation system (Beckman Coulter Inc. ...
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bioRxiv - Bioengineering 2022Quote: Cu and Zn ion release from PCu coupons (surface area 5 cm2) and Cu-coated SS coupons was measured using inductively coupled plasma optical emission spectrometry (ICP-OES, Perkin Elmer, Optima 8300). The PCu coupons were wet ground with a 600 grit SiC emery paper and rinsed with DI water ...
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bioRxiv - Neuroscience 2022Quote: ... or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer, Waltham, MA, USA) at 30°C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Bioluminescence imaging was performed at days 5 and 7 post engraftment using an IVIS Lumina III in vivo imaging system (Perkin Elmer, Waltham, MA). IVIS instrument exposure time was 3 seconds ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 5-HT uptake was initiated by the addition of 10 nM [3H] 5-HT (Cat No: NET498001MC; 24.82 Ci/mmol: Perkin Elmer, Santa Clara, CA). and the uptake was terminated at 5 min with the addition of fluoxetine followed by rapid filtration over GF-B filters ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µM cycloheximide and 10 µCi ml-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: [3H]-DHA (1-[4,6-propyl-3H]dihydroalprenolol, specific activity 91 Ci·mmol-1) was obtained from PerkinElmer Life and Analytical Sciences (Beaconsfield ...
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bioRxiv - Microbiology 2021Quote: ... spiked with 500 nmol 3H-leucine L-1 (Perkin Elmer, specific activity 100 Ci mmol- 1) and incubated in the dark for 4 h ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µM cycloheximide and 10 µCi mL-1 Na-14C acetate (PerkinElmer: 56.6 mCi mM-1) were then added to the culture for the 7-min pulse ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and ATPlite 1 Step (Perkin Elmer). The main instruments used were the microplate reader SpectraMax 340PC (Molecular Device ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 650 (1:1500, PerkinElmer, FP1496001KT), Opal 690 (1:1500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 690 (1:1500, PerkinElmer, FP1497001KT). Following signal amplification of the target probes ...
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bioRxiv - Cell Biology 2022Quote: ... 1:750 TSA Cy3 (#NEL704A001KT, PerkinElmer) and TSA Cy5 (#NEL75A001KT ...
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bioRxiv - Neuroscience 2021Quote: ... Opal 570 (1:1500, Perkin Elmer), and Opal 690 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μCi [14C]glycerol (Perkin Elmer) + 50 μM glycerol to stimulate glycerolipid synthesis ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μM [3H]-SAM (Perkin Elmer), 1 μM full-length C ...
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bioRxiv - Biophysics 2023Quote: ... 1 μM 3H-L-Asp (PerkinElmer), and 0.5 μM valinomycin ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL γ-32P-ATP (PerkinElmer) and 10 U T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence polarization measurements were carried out in black 96-well plates measured on a Wallac Victor 2 Plate Reader (Perkin Elmer). For all measurements peptide was held at concentrations of 50 nm (or 100nM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were dried for at least 30 mins at 95 °C to remove unfixed 14C before re-suspending the fixed 14C pellets with ultra-pure water and adding 2 mL of scintillation cocktail (Ultima Gold XR, PerkinElmer, US). Radioactivity measurements were then performed using a scintillation counter (Tri-Carb ...
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bioRxiv - Cell Biology 2021Quote: ... 500nl of 2 µM siRNAs (20nM final concentration) were distributed on 6 different 384-well imaging plates (CellCarrier Ultra, Perkin Elmer), as described in Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... Cytotoxicity was measured by adding resazurin [alamar blue (ThermoFisher)] diluted to 1x in DMEM according to the manufacturor’s protocol and incubated on cells for 2-4hr and reading fluorescence on a Victor 1420 Multilabel plate reader (Perkin Elmer) with excitation at 560 nM and emission at 590 nM.
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in a solution containing an isotopic dilution of 2 μCi/ml [3H] glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the amount of tritiated acetate in the organic phase was measured by liquid scintillation counting for 2 min (Tri-Carb 2900TR, Perkin Elmer). Each sample was assayed in triplicate and counts were corrected for background by subtraction of counts obtained using whole blood samples devoid of cholinesterase activity and treated in a similar fashion ...
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bioRxiv - Physiology 2020Quote: On the fifth day of EOS or PBS treatment 3H-2-deoxy-D-glucose (2DG; 10 uCi/mouse; PerkinElmer, Waltham, MA) was given to 4-hour fasted individually housed mice ...
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bioRxiv - Molecular Biology 2020Quote: Nascent proteins in U-2 OS cells expressing GFP-G3BP1 were labeled with 35S-met and -cys (EXPRE35S35S Protein Labeling Mix, Perkin Elmer) for 30 minutes in the presence of DMSO (0.1%) ...
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bioRxiv - Developmental Biology 2021Quote: ... Successfully mounted embryos were then imaged starting between 2 and 7 ss using a 20X objective on a Perkin-Elmer UltraVIEW Vox spinning disk confocal microscope (Perkin Elmer) while maintained at 32°C ...
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bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
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bioRxiv - Microbiology 2020Quote: Infected mice were anesthetized using isofluorane and 1 μl of decyl aldehyde was instilled into each nostril before placement in a BSL-2 chamber within the IVIS instrument (IVIS Spectrum, Perkin Elmer). Isofluorane anesthesia was sustained during IVIS imaging ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was separated in 2% glyoxal agarose gels and blotted onto a positively charged nylon membrane (GeneScreen Plus, Perkin Elmer). [32P]-labelled probes specific for each transcript were generated by linear PCR ...
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bioRxiv - Cell Biology 2020Quote: ... subconfluent HDLECs cultured in 6-well plates were incubated with 2 ml per well EBM2 medium (containing appropriate amounts of serum and supplement) with [14C(U)]-glutamine (Perkin Elmer) for 6 hours ...
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bioRxiv - Immunology 2021Quote: ... Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Physiology 2023Quote: ... the animals were fasted overnight and a bolus of 2-deoxy-D-[1,2-3H]-glucose (1mC/ml; specific activity: 20Ci/mmol; PerkinElmer, Madrid, Spain) mixed with glucose (100µCi/kg body weight ...
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bioRxiv - Biochemistry 2023Quote: ... 690 uL of the samples were transferred to a scintillation vial with 2 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer). Radioactivity was measured with the Scintillation counter (Perkin Elmer) ...
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bioRxiv - Microbiology 2024Quote: ... 2×106 bacteria/mL in PBS were seeded in 384-well black clear-bottom plate (ViewPlate®-384 F TC, PerkinElmer) in the presence of 12.5 % BRC ...
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bioRxiv - Neuroscience 2022Quote: ... 1:200 in TNB buffer or NGS for 2 hours at room temperature and then incubated with HRP-Conjugated Streptavidin (PerkinElmer; USA) 1:100 in TNB buffer or NGS for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured for 90 minutes at 2-min intervals using a 340/460 excitation/emission filters on a Envision plate reader (Perkin Elmer). The IC50 values were calculated by fitting the curves using a four-parameter equation in GraphPad Prism ...
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bioRxiv - Microbiology 2022Quote: ... Cells (2 × 104 cells/well) were seeded into 96-well plates (Cell Carrier-96well Ultra microplate; PerkinElmer Inc., Waltham, MA, USA) and differentiated to macrophages with phorbol 12-myristate 13-acetate (10 ng/mL ...
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bioRxiv - Biochemistry 2022Quote: ... A competitor unlabelled DNA of the same sequence was added at 2 µM and FP was measured over time on a VictorV plate reader (Perkin Elmer). Experiments with PARP1 were carried out in the same conditions as for PARP2 with the exception that the reaction buffer which was 12 mM Hepes pH 8.0 ...