Labshake search
Citations for PerkinElmer :
951 - 973 of 973 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Extracted DNA was sent to Texas A&M Agrilife Bioinformatics and Genomics Service (College Station, TX, USA) for whole-genome library preparation using a NEXTFLEX Rapid XP DNA-Seq Kit HT (PerkinElmer, Waltham, MA, USA). Libraries were sequenced at the Texas A&M Agrilife Bioinformatics and Genomics Service on one NovaSeq 6000 S4 lane at 26.1 ± 5.8 depth of coverage per sample across the full genome (2 s.d. ...
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bioRxiv - Microbiology 2022Quote: Quality and quantity of the extracted RNA samples were analyzed with LabChip GX Touch HT RNA Assay Reagent Kit (PerkinElmer, Waltham, MA, USA) and Qubit RNA BR kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN) of 9.5 using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit (PerkinElmer®, Waltham, MA). Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Genomics 2022Quote: ... 1 ng of eluted and purified DNA was used to prepare libraries with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, #NOVA-5188-01) on the Illumina NextSeq500 system using a single read 100 bp protocol ...
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bioRxiv - Biochemistry 2023Quote: The expression of IL-6 and TNF-α in culture supernatants was detected using the corresponding AlphaLISA Immunoassay Kit (PerkinElmer, AL223C and AL208C) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2024Quote: The staining and fixation were performed following the published protocol (Cimini et al., 2023) with the use of PhenoVue JUMP kit (Perkin Elmer, ref.: PING23). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: Intracellular ATP and glutamine/glutamate levels were measured for each NCC primary cultures after Seahorse analysis using the ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943), and the Glutamine/Glutamate-Go assay (J8021 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents, Perkin Elmer TSA Plus Cy3 or Cy5 Kit). Residual enzyme activity was inhibited via 45-minute incubation in 0.1% hydrogen peroxide in PTW followed by four PTW washes prior to addition and development of the second peroxidase-conjugated antibody76.
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bioRxiv - Biochemistry 2020Quote: ... Library preparation for deep sequencing was performed using the NextFlex Rapid Directional qRNA-Seq Library Prep Kit (Perkin Elmer, Bioo Scientific, NOVA-5130-01D) according to the manufacturer’s instructions using 7 unique barcodes ...
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bioRxiv - Microbiology 2019Quote: ... The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2×51 cycles on an Illumina HiSeq 2000 system (Illumina ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was fragmented with a COVARIS S2 and an Illumina-compatible library was prepared with the NEXTflex Bisulfite Library Prep Kit for Illumina Sequencing (Bioo Scientific/PerkinElmer, Austin, Texas, U.S.A). Bisulfite conversion was performed using the EZ DNA Methylation Gold Kit (Zymo Research ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ligand-stimulated cAMP accumulation in the presence or absence of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) was measured after the indicated times of stimulation using LANCE® cAMP Detection Kit (Perkin Elmer Life Sciences) and a Mithras LB 940 multimode microplate reader ...
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bioRxiv - Cell Biology 2022Quote: ... determined by competitive immunoanalysis using Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) as a detection system (Lance Ultra cAMP kit from Perkin Elmer, Waltham, MA, USA) according to the manufacture instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...
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bioRxiv - Physiology 2024Quote: ... Oxytocin concentrations in the saliva samples were measured in duplicate and calculated following the manufacturer’s instructions utilizing the Oxytocin ELISA Kit Wako (FUJIFILM Wako Pure Chemicals Corp., Japan) and a plate reader (EnSpire® Multimode Plate Reader, PerkinElmer, Inc., Massachusetts, USA).
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bioRxiv - Genetics 2024Quote: ... RNA quality was assessed in an Agilent 2100 Bioanalyzer and strand-specific cDNA libraries were made with NEXTflex™ Rapid Directional qRNA-Seq™ Kit (PerkinElmer, formerly BiooScientific) with molecular indexing ...
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bioRxiv - Microbiology 2023Quote: ... Compounds were incubated for 48 h and cell viability was determined by measuring the adenosine triphosphate (ATP) content of the cells using the ATPlite kit (PerkinElmer, Waltham, MA, Cat: 6016941) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared from the 10.5 μl reactions using the NEXTflex Small RNA-Seq Kit v3 (Perkin Elmer/Bioo Scientific, #NOVA-512-05) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Illumina libraries were prepared using the ResolveOME library preparation kit (BioSkryb Genomics Inc.) with NEXTFLEX Unique Dual Index Barcodes (PerkinElmer Applied Genomics, NOVA-534100). Libraries were sequenced at low-pass (2x 50bp paired end ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.