Labshake search
Citations for PerkinElmer :
851 - 900 of 973 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA extraction from K2-EDTA-blood tubes was performed with chemagic 360 instrument and the CMG-704 kit (PerkinElmer), which uses magnetic bead technology ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... The cortisol ELISA assay was performed as per manufacturer’s instructions using the Cisbio HTRF cortisol ELISA kit (62CRTPEG, Cisbio, Perkin Elmer) and measurements were performed on Tecan Infinite M1000 Pro multi-plate reader.
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bioRxiv - Microbiology 2022Quote: ... Quality of the libraries was measured with LabChip GX Touch HT DNA High Sensitivity Reagent Kit (PerkinElmer, Waltham, MA, USA). Sequencing was performed with NovaSeq 6000 System (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: RNA purified from the IPs and ribodepleted RNA from the input samples were processed for library preparation with the Nextflex Small RNA kit v3 (#NOVA-5132-06, PerkinElmer), following manufacturer instructions ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescent signals corresponding to the target transcript were generated using the Tyramide Signal Amplification (TSA) Plus Fluorescein Kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of nascent RNA was used as input to construct plaNETseq libraries using NEXTflex Small RNA-seq kit v3 (PerkinElmer) with a modified protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Free amino acids were determined with the EZ:FAAST amino acid analysis kit of Phenomenex on a GC-FID (Perkin Elmer) according to supplier instructions ...
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Molecular Biology 2023Quote: ... reagents and conditions of the BigDye Terminator v3.1 Cycle sequencing Kit and an automated capillary sequencer ABI 3700 DNA analyzer (Perkin Elmer, Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... The slides were subjected to sequential staining cycles as described before,3 although now automated using Opal 7-color Automation IHC Kit (NEL801001KT; PerkinElmer) on the BOND RX IHC & ISH Research Platform (Leica Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IP1 levels were determined using the Cisbio HTRF® IP-one assay kit per the manufacturer’s instructions and measured using an Envision plate reader (PerkinElmer). Data are expressed as fold of basal IP1 levels.
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bioRxiv - Genetics 2023Quote: ... 10 µg of total RNA was polyA-selected and processed using the NEXTFLEX Rapid Direction RNA-seq Kit (NOVA-5138-10 and NOVA-5138-11; PerkinElmer) according to the provided manual ...
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bioRxiv - Neuroscience 2023Quote: Amplified Luminescent Proximity Homogeneous Assay Screen (ALPHAScreen) was carried out utilising the cMyc detection kit and Proxiplate-384 Plus plates (PerkinElmer) as previously described (Martin et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and an aliquot from the supernatant was collected to quantify cAMP using the LANCE cAMP kit (Perkin Elmer, cat#AD0262) according to the manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer, Austin, Texas, USA). Quality of library preparation was assessed on a 2100 Agilent Bioanalyzer ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were prepared from 33ng of genomic DNA using a custom miniaturized version of the NEXTFLEX Rapid XP kit protocol (PerkinElmer) that was automated on a Sciclone NGSx liquid handler (PerkinElmer) ...
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bioRxiv - Plant Biology 2023Quote: ... RPFs were directly used as input into the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06) or V4 (Perkin Elmer ...
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bioRxiv - Plant Biology 2024Quote: ... the fragmented gDNA was constructed into sequencing libraries using a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA). The full-length coding sequence of ZmHsf20 was cloned into the pFN19K HaloTag T7 SP6 Flexi vector ...
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bioRxiv - Cell Biology 2024Quote: ... using a HyBEZ® oven Probes were detected using Tyramide signal amplification (TSA) reaction (1:600 dilution) using an Alexa-flour labeled TSA kit (PerkinElmer) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... before incubated with the Fluorophore Tyramide Amplification Reagent (1:200 in amplification diluent, TSA Plus multi-fluorophore detection kit, Fluorophore: Fluorescein, PerkinElmer, NEL753001KT). Sections were washed overnight in 1x PBS at RT ...
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... All treated and RNA-extracted samples were then used to produce small libraries for sequencing using a NETFLEX small RNA-Seq kit (PerkinElmer, USA).
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bioRxiv - Neuroscience 2019Quote: ... Phosphorylation was determined by alpha-screen bead-based technology using the Amplified Luminiscent Proximity Homogeneous Assay kit (PerkinElmer, Waltham, MA, USA) and the Enspire Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... Final library products were assessed for quality on LabChip GX using the DNA High Sensitivity Reagent Kit (Perkin Elmer, MA, USA), diluted to 2 nM concentration and pooled ...
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bioRxiv - Microbiology 2020Quote: ... Stool samples underwent DNA extraction using chemical and mechanical lysis with magnetic bead-based purification via the Chemagic MSM I with the Chemagic DNA Blood Kit-96 from Perkin Elmer. Prior to extraction on the MSM-I ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting double-stranded cDNA was then used to construct an Illumina-compatible sequencing library (BioO NextFlex RNA Library Kit, Perkin Elmer). Libraries were quantified using qPCR (NEBNext Library Quantification ...
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bioRxiv - Genomics 2019Quote: ... Probes were then amplified and the HRP signal was developed using TSA Fluorescein Plus Evaluation Kit (NEL741E001KT, Perkin Elmer, Waltham, MA). Finally ...
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bioRxiv - Physiology 2019Quote: ... and stimulated with either GLP-1 or GIP for 30 minutes prior to measurement of cAMP accumulation using a LANCE® Ultra cAMP Detection Kit (PerkinElmer) in accordance with the manufacturer’s instructions and as described previously (61 ...
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bioRxiv - Neuroscience 2019Quote: ... Detection was visualized with tyramide-488 1:50 in supplied amplification buffer from the TSA-Plus in situ kit (Perkin Elmer).
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA from parents and progenies was extracted from 100 mg of frozen young leaves with the Chemagic DNA Plant Kit (Perkin Elmer Chemagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Ligand-induced ERK1/2 phosphorylation was assessed using the AlphaLISA Surefire Ultra p-ERK1/2 (Thr202/Tyr204) kit (PerkinElmer, Melbourne, Australia) following the manufacturer’s protocol as previously described (23 ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA concentration was measured with Quant-iT™ PicoGreen™ dsDNA Assay Kit after it was eluted in 400µl 10mM Tris-EDTA elution buffer (PerkinElmer). Aliquotes of DNA samples were produced with Tecan Genesis/Tecan Freedom Evo and then shipped on dry ice for genetic analyses ...
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bioRxiv - Cancer Biology 2022Quote: ... Media supernatants of the 4T1 cells in the plates were collected and immediately analyzed with ATPlite one-step luminescence assay kits (PerkinElmer, MA) for the measurement of ATP concentration in the media ...
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bioRxiv - Cell Biology 2022Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Perkin Elmer (Waltham, MA), per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: The AlphaLISA pSTAT1 assay was performed using the AlphaLISA SureFire Ultra p-STAT1 (Tyr701) assay kit (PerkinElmer, Inc., Waltham, MA, USA). HeLa cells (CCL-2 ...
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested post-stimulation and the production of TNF-α was assessed using the AlphaLISA Immunoassay Kit (PerkinElmer, AL208C) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...