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Citations for PerkinElmer :
51 - 100 of 468 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells stably transfected with LRRK2 plasmids were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... cells were lysed and supernatants were analyzed with phosphoSyk Alpha-LISA kit (PerkinElmer, Waltham, MA, USA) according to the kit instructions ...
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bioRxiv - Microbiology 2021Quote: The AlphaLISA assay development was performed in 96-well ½area Alpha plate (Perkin Elmer, Waltham, MA) with a final reaction volume of 40 µL ...
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bioRxiv - Molecular Biology 2024Quote: The Amplified Luminescent Proximity Homogeneous Assay (ALPHA Assay) was performed in white 384-well Optiplates (PerkinElmer) in a final volume of 20 μl ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Systems Biology 2023Quote: ... The NPCs were cultured in a 12-well dish to confluency in NPC media and then dissociated using Accutase for 5 minutes at 37°C to single cell resolution and plated on 5 µg/mL human laminin and 5µg/mL fibronectin-coated 384 well plates (Perkin Elmer Cat #6057308) at 12,000 cells/well in NPC media and 10 µM ROCKi ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth (OD600) was determined approximately 20 h later utilizing a Enspire Alpha multimode plate reader (PerkinElmer). To calculate the MIC90 ...
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bioRxiv - Biochemistry 2022Quote: ... and the change in tryptophan fluorescence associated with the exchange of guanyl nucleotides within the α subunit of Gt was measured for 1200 s with a FL 6500 Fluorescence Spectrometer (PerkinElmer, Waltham, MA). Excitation and emission wavelengths were set at 300 nm and 345 nm ...
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bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... The emitted signal intensity was measured using standard Alpha settings on an EnSpire® Multimode Plate Reader (PerkinElmer). Measurements are expressed as technical duplicates (mean+/- SD ...
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bioRxiv - Cancer Biology 2023Quote: ... an Alpha SureFire Ultra Multiplex Phospho-EGFR (Tyr1068) and Total EGFR Assay Kit (PerkinElmer MPSU-PTEGFR-K-HV) was used ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Molecular Biology 2024Quote: ... from synthetic oligonucleotides (0.2 µM) and trace-labeled with 32P alpha-ATP (800 Ci/mmol, 12.5 µM, Perkin Elmer) in a 20 µl reaction according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular culture media were collected in order to dose secreted Aβ using Alpha-LISA assays (AlphaLISA Amyloid β1-X Kit, AL288C, PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... to a fixed concentration of target (e.g., biotinylated PTM-defined nucleosome) with the appropriate Alpha Donor and Acceptor beads (Perkin Elmer) (Jain et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the same procedure for the cold transcription except for the use of 0.5 µl alpha-32P-labeled UTP (3000 Ci/mmol-10 mCi/ml) (Perkin Elmer) and 0.8 mM UTP (instead of 2 mM) ...
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bioRxiv - Immunology 2024Quote: ... with fluorescence detected on intensity (485 nm excitation and 530 nm emission) measured by an EnSpire Alpha Multimode Plate Reader (PerkinElmer).
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bioRxiv - Biochemistry 2024Quote: ... For competition binding experiments cell membranes expressing h5-HT3A WT or mutant receptors were incubated in 250 µL 10 mM HEPES buffer (pH 7.4) containing 0.3 nM [3H]-GR65630 (Perkin Elmer, Waltham, MA) and varying concentrations of VTX or analogues ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were read at 450 nm and readings were processed to observe binding patterns of tested molecules (Perkin Elmer, Enspire Alpha). For every experiment ...
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bioRxiv - Neuroscience 2019Quote: ... Phosphorylation was determined by alpha-screen bead-based technology using the Amplified Luminiscent Proximity Homogeneous Assay kit (PerkinElmer, Waltham, MA, USA) and the Enspire Multimode Plate Reader (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were initiated by the addition of GTP to a final concentration of 40 uM containing a trace amount of [alpha-32P]-GTP (Perkin Elmer) and incubated for 20 min at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Cancer Biology 2019Quote: ... AlphaLISA assays were performed in 96-well half area white plates (6005560) and read in an EnSpire Alpha 2300 Mutilabel Plate reader (Perkin Elmer, Inc.). A 96-well white plate was filled with 5 µl of EVs or 10 µl of CM ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Saturation binding assay was performed to quantify the receptor expression and equilibrium dissociation constant of the radioligand [3H]-NMS (PerkinElmer, specific activity 80 Ci/mmol). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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bioRxiv - Microbiology 2023Quote: ... The 14C bicarbonate stock solution (aqueous, activity 5 mCi/5 mL, PerkinElmer, Inc.) was diluted to a final specific activity of 5 µCi/50µL and added to the groundwater sample (43 mL ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Microbiology 2021Quote: ... 5-6[3H] (Perkin Elmer) (6 µCi/mL) ...
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bioRxiv - Developmental Biology 2021Quote: ... and cyanine 5 (PerkinElmer #NEL745E001KT). Sections were then incubated in 1X Trueblack Lipofuscin Autofluorescence Quencher (VWR #10119-144 ...
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bioRxiv - Neuroscience 2022Quote: ... D-[5-3H(N)] (PerkinElmer). Cells were incubated for 4 and 6 h after which cell supernatants from duplicate plates were collected at each respective time-point and stored at −20 °C ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Microbiology 2021Quote: ... 5 μCi 3H-adenosine (Perkin Elmer), or 10 μM EdA was supplemented ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi [3H]OA (Perkin Elmer) + 0.4 mM OA/0.5% BSA complex ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μCi [γ-32P] ATP (PerkinElmer) and 10 units T4 PNK enzyme (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µCi/ml [3H]-cholesterol (PerkinElmer) was added in the presence of 10% FBS ...