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Citations for PerkinElmer :
51 - 100 of 1277 citations for 7 Benzyl 4 chloro 5 6 7 8 tetrahydropyrido 3 4 d pyrimidin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The sections were labeled by using the Opal 7-Color fIHC Kit (PerkinElmer, Waltham, MA). The antibody fluorophores were grouped into a panel of 5 antibodies ...
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bioRxiv - Microbiology 2022Quote: A549 or Huh-7 cells were seeded in 96-CellCarrierUltra plates (Perkin Elmer, Hamburg, Germany), then infected or treated as described above ...
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bioRxiv - Developmental Biology 2020Quote: ... Opal570 (1:500) and Opal650 (1:1500) using the Opal 7 Kit (NEL797001KT; Perkin Elmer).
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bioRxiv - Immunology 2022Quote: ... Immunofluorescent signal was visualized using the OPAL™ 7-color fIHC kit (Perkin Elmer, MA) TSA dyes 480 ...
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bioRxiv - Cancer Biology 2023Quote: ... and visualized using Tyramide Signal Amplification for 10 min (Opal 7-Colour IHC, Perkin Elmer). Sections were stained with DAPI for 5 min and mounted using ProLong® Diamond ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Developmental Biology 2021Quote: ... 3-D reconstruction was performed with Volocity (PerkinElmer).
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bioRxiv - Neuroscience 2020Quote: ... 3 and 6 hr later cells were labeled for 5 min with 1 mCi/ml 35S Protein Labeling Mix (PerkinElmer). Lysates from 25-ml culture triplicate samples were analyzed by SDS-PAGE and autoradiography.
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bioRxiv - Immunology 2019Quote: ... Mono-color staining of each of the antibodies was performed using the OPAL 7 color kit (PerkinElmer), using the same protocol as IHC ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue samples were then stained using the PerkinElmer OPAL 7 Color Automation Immunohistochemistry Kit (PerkinElmer, Waltham, MA) on the BOND RX Autostainer (Leica Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... Staining was performed employing tyramide signal amplification (TSA) and the OpalTM 7-color Manual IHC Kit (PerkinElmer). To prevent unspecific binding of antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Multispectral fluorescence immunohistochemistry (mfIHC) assays were performed using a PerkinElmer Opal™ 7-color mfIHC Kit (PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... The multiplexed IF staining was performed following the instruction of Opal 7-color IHC kit (NEL811001KT, PerkinElmer). Other antibodies and staining reagents used in this study include rabbit anti-smooth muscle actin (SMA ...
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bioRxiv - Neuroscience 2023Quote: ... 7 increasing concentrations (0.15 – 10 nM) of [3H]-SCH23390 (specific activity 83.6Ci/mmol, Perkin Elmer, #NET930025UC, Canada) for D1 binding to generate saturation curves ...
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bioRxiv - Immunology 2019Quote: ... After 4-6 washes with 1x TBST the membrane was treated with chemiluminescent substrate Western Lightning ECL Pro (Perkin Elmer; NEL120001EA) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 4 μL/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 μL/well of ATPLite (PerkinElmer) was dispensed ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl/well of ATPLite (PerkinElmer) was added to assay plates and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or a linear OXTR antagonist [125I]-d(CH2)5[Tyr(Me)2-Tyr-Nh2]9-OVT (Perkin Elmer, USA) as tracers ...
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bioRxiv - Cancer Biology 2021Quote: ... Multispectral immunohistochemistry was performed using the Opal 7-Color Manual IHC Kit (Akoya Biosciences/PerkinElmer, NEL811001KT, Marlborough, MA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were monitored every 3-4 days by non-invasive luciferase imaging by intraperitoneal injection of 150 mg/kg Luciferin (PerkinElmer) and quantification of luciferase activity using Imaging Systems (IVIS Spectrum System ...
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bioRxiv - Biochemistry 2021Quote: Structural models of polyenoyl tetramic acids 3 and 4 were built and energy minimized using Chem3D v.16.0 (Perkin Elmer). The terminal region of polyenes ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µL of RNA was 3’-end-labeled with 32pCp (prepared by incubating 16.5 µl of γ-32P-ATP (PerkinElmer), 1 µl of T4 polynucleotide Kinase ...
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bioRxiv - Immunology 2019Quote: ... cyanine 3 and cyanine 5 (Perkin Elmer). The ACD 3-plex negative control probe was run in parallel on separate sections in each experiment to assess the background level and set the acquisition parameter ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were split again on days 4 or 5 as needed into a 96-well imaging plate (Perkin Elmer). A half media change was performed every other day if cells were not being split ...
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bioRxiv - Molecular Biology 2021Quote: ... The medium was removed and 125I-GLP-1(7-36)NH2 (60 pM) or 125I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled tirzepatide or peptide 20 were added for overnight incubation at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... A fragmentation step was carried out for 6.5 minutes at 94°C and 7 μM NextFlex DNA barcodes (PerkinElmer) were used for adapter ligation ...
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bioRxiv - Cell Biology 2023Quote: ... by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC, PerkinElmer) for 1 h at 30 °C at 600 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μL 14C-labelled lipids from the denser organic fraction were combined with 4 mL OptiPhase HiSafe 3 liquid scintillation cocktail (PerkinElmer; 1200.437) and radio-labeling was quantified using a Tri-Carb 2910 TR Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Plant Biology 2020Quote: ... For all assays 1 μCi [3 H]-D-glucose (PerkinElmer, USA) was used as the radioactive tracer ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Each condition was imaged from duplicated wells and a minimum of 7 fields/well using Operetta high-content imaging system 1483 (PerkinElmer). The measurement of p16 and p21 fluorescence intensity per area (normalised to the number of cells ...
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bioRxiv - Neuroscience 2021Quote: ... after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU) by the reaction was read with a fluorimeter (EnSpire Plate Reader, PerkinElmer) selecting an excitation wavelength of 365 nm and an emission wavelength of 445 nm ...
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bioRxiv - Bioengineering 2022Quote: ... Animals were kept under anesthesia (3-4%) following CHP injection and an initial image acquisition was performed using an IVIS Spectrum imaging system (PerkinElmer, Waltham, MA) <15 minutes following injection ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... the Opal 4-colour IHC kit (Perkin Elmer) was used per manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... 4 µl of [γ-32P] ATP (PerkinElmer, #BLU502A001MC), and 10 units of T4 polynucleotide kinase (PNK ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μL of the acceptor beads (Perkin Elmer) diluted in PBS was added to each well in the dark and incubated for 90 mins ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μL of the donor beads (Perkin Elmer) diluted in PBS was added to each well to give a total volume of 25 μL and incubated for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded overnight with MEM +HIHS cell culture media supplemented with 4 μCi/mL D-[2,3-3H]aspartate (Perkin Elmer, Waltham, MA, cat. #NET501001MC). Coverslips were washed in isosmotic Basal medium containing (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... 50 nM of hNatD was mixed with 2 μM of radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences), 20 μM of the peptide ...
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bioRxiv - Immunology 2019Quote: ... Mice were injected with 3 milligrams (200 µl) of D-luciferin (PerkinElmer) substrate ...
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bioRxiv - Microbiology 2019Quote: ... After enzymatic conversion the fluorescent signals corresponding to the amount of released 4-methylumbelliferone (4-MU) were quantified in a microplate reader (Victor X2, Perkin Elmer) with the excitation and emission wavelengths of 355 and 460 nm ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted 1:500 in carbonate buffer pH 9.7 (25 mM sodium bicarbonate, 25 Mm sodium carbonate) and coated in flat-bottom white plates (OptiPlate 96F-HB, Perkin Elmer) overnight at 4°C under agitation ...
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bioRxiv - Neuroscience 2023Quote: ... in the presence of 7 increasing concentrations (0.15 – 10 nM) of [3H]-raclopride (specific activity 82.8Ci/mmol, Perkin Elmer, #NET975250UC, Canada) for D2 binding ...
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bioRxiv - Cancer Biology 2021Quote: ... Image acquisition was performed using Volocity versions 5 and 6 (PerkinElmer). Routine imaging performed using Plan Apochromat 60 or 100× oil immersion objectives ...
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bioRxiv - Genetics 2020Quote: ... or 6 μM of Texas Red-5-dATP (Perkin Elmer, NEL471001EA) in 800 μl of 1 × NEBuffer 2 and carried out at 37°C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μCi of 2-Deoxy-D-[1-2-3H] glucose (PerkinElmer, Boston, MA, USA # NET328A001MC) and 10μM non-radioactive 2-DG (Sigma Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mm root segments away from the apical 2 mm were carefully cut and soaked for overnight in 4 mL liquid scintillation fluid (ULTIMA GOLD, PerkinElmer, USA), and the radioactivity was measured with a scintillation counter (model LS6500 ...