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Citations for PerkinElmer :
801 - 843 of 843 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Plant Biology 2023Quote: ... These purified ribosome footprints were used as input for the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06). The resulting cDNA was amplified by 11-16 cycles of PCR with a barcode incorporated in the primer ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
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bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Cell Biology 2021Quote: ... the RBP-RNA complex was purified and the bound RNA eluted for subsequent Illumina library preparation (NEXTFLEX small RNA library preparation kit v3, PerkinElmer #NOVA-5132-06) and paired-end (40×43 cycles ...
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bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular ATP and extracellular lactate levels were measured for each NCC primary cultures after Seahorse analysis using an ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943) and the Lactate Fluorometric Assay kit from BioVision (cat ...
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bioRxiv - Physiology 2020Quote: ... 1 ng of eluted and purified DNA was used to prepare DNA sequencing library with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, NOVA-5188-01) on the NextSeq 500 system (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... we followed the protocol provided by the commercial company except that all samples were stained in 1 to 80 dilution of Cy3-conjugated or FLU-conjugated Tyrimide substrates for 60 minutes (Perkin Elmer NEL753001 Kit).
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bioRxiv - Genetics 2020Quote: ... and used as input for small RNA library prep using the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific - PerkinElmer NOVA-5132-06). Libraries were prepared according to manufacturer’s instructions (V19.01) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA hybridization were performed on dechorionated 20 hpf embryos with the tyramide amplification kit (TSA Plus Cyanine 3 System, Perkin Elmer, Boston, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... often from an ImmPRESS® polymer detection kit followed by colour development using a tyramide signal amplification system kit with cyanine (Cy)3 or fluorescein (1:50 dilution; PerkinElmer, Waltham, MA, USA). For detection of the second antigen in dual immunofluorescence ...
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bioRxiv - Microbiology 2021Quote: DNA library preparation was carried out according to the manufacturer’ ss protocol using the NEXTflex™ ChIP-Seq Kit (NOVA-5143-01) (PerkinElmer, Hamburg, Germany) and the NEXTflex™ ChIP-Seq Barcodes (NOVA-514122 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Extracted DNA was sent to Texas A&M Agrilife Bioinformatics and Genomics Service (College Station, TX, USA) for whole-genome library preparation using a NEXTFLEX Rapid XP DNA-Seq Kit HT (PerkinElmer, Waltham, MA, USA). Libraries were sequenced at the Texas A&M Agrilife Bioinformatics and Genomics Service on one NovaSeq 6000 S4 lane at 26.1 ± 5.8 depth of coverage per sample across the full genome (2 s.d. ...
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bioRxiv - Microbiology 2022Quote: Quality and quantity of the extracted RNA samples were analyzed with LabChip GX Touch HT RNA Assay Reagent Kit (PerkinElmer, Waltham, MA, USA) and Qubit RNA BR kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN) of 9.5 using the NEXTFLEX® Combo-Seq mRNA/miRNA Kit (PerkinElmer®, Waltham, MA). Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...
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bioRxiv - Genomics 2022Quote: ... 1 ng of eluted and purified DNA was used to prepare libraries with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, #NOVA-5188-01) on the Illumina NextSeq500 system using a single read 100 bp protocol ...
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bioRxiv - Biochemistry 2023Quote: The expression of IL-6 and TNF-α in culture supernatants was detected using the corresponding AlphaLISA Immunoassay Kit (PerkinElmer, AL223C and AL208C) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Evolutionary Biology 2019Quote: ... followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents, Perkin Elmer TSA Plus Cy3 or Cy5 Kit). Residual enzyme activity was inhibited via 45-minute incubation in 0.1% hydrogen peroxide in PTW followed by four PTW washes prior to addition and development of the second peroxidase-conjugated antibody76.
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bioRxiv - Biochemistry 2020Quote: ... Library preparation for deep sequencing was performed using the NextFlex Rapid Directional qRNA-Seq Library Prep Kit (Perkin Elmer, Bioo Scientific, NOVA-5130-01D) according to the manufacturer’s instructions using 7 unique barcodes ...
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bioRxiv - Microbiology 2019Quote: ... The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2×51 cycles on an Illumina HiSeq 2000 system (Illumina ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was fragmented with a COVARIS S2 and an Illumina-compatible library was prepared with the NEXTflex Bisulfite Library Prep Kit for Illumina Sequencing (Bioo Scientific/PerkinElmer, Austin, Texas, U.S.A). Bisulfite conversion was performed using the EZ DNA Methylation Gold Kit (Zymo Research ...
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bioRxiv - Cancer Biology 2021Quote: ... For multiplexed immunohistochemistry signals were detected using Opal™ 4-Plex Kit according to the manufacturer’s protocol (catalog no. NEL810001KT; Perkin Elmer, Waltham, MA, USA). For more details see Supplemental Materials section.
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Compounds were incubated for 48 h and cell viability was determined by measuring the adenosine triphosphate (ATP) content of the cells using the ATPlite kit (PerkinElmer, Waltham, MA, Cat: 6016941) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Pelleted particles were resuspended in D10 medium and the concentration of HIV p24 was quantitated by using an HIV-1 p24 ELISA Kit (Perkin Elmer, Boston, MA, USA) [44 ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared from the 10.5 μl reactions using the NEXTflex Small RNA-Seq Kit v3 (Perkin Elmer/Bioo Scientific, #NOVA-512-05) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Illumina libraries were prepared using the ResolveOME library preparation kit (BioSkryb Genomics Inc.) with NEXTFLEX Unique Dual Index Barcodes (PerkinElmer Applied Genomics, NOVA-534100). Libraries were sequenced at low-pass (2x 50bp paired end ...
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bioRxiv - Physiology 2024Quote: ... Oxytocin concentrations in the saliva samples were measured in duplicate and calculated following the manufacturer’s instructions utilizing the Oxytocin ELISA Kit Wako (FUJIFILM Wako Pure Chemicals Corp., Japan) and a plate reader (EnSpire® Multimode Plate Reader, PerkinElmer, Inc., Massachusetts, USA).
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...
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bioRxiv - Genetics 2024Quote: ... RNA quality was assessed in an Agilent 2100 Bioanalyzer and strand-specific cDNA libraries were made with NEXTflex™ Rapid Directional qRNA-Seq™ Kit (PerkinElmer, formerly BiooScientific) with molecular indexing ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration was measured using the Picogreen method (Invitrogen Quant-iT™ Picogreen dsDNA Assay Kit Reference No. P11496 on Perkin Elmer 2030 Multilabel Reader Victor X3) and DNA integrity number (DIN ...