Labshake search
Citations for PerkinElmer :
751 - 800 of 893 citations for hsa mir 373 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the converted DNA was then used for the library preparation using the NEXTFLEX® Bisulfite-Seq kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from standard cultures were prepared according to manufacturer’s protocol using the NextFlex Combo-Seq mRNA/miRNA kit (PerkinElmer), to capture both the mRNA and miRNA in the samples ...
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bioRxiv - Cell Biology 2023Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Physiology 2023Quote: ... and its integrity was assessed using 5K / RNA / Charge Variant Assay LabChip and RNA Assay Reagent Kit (Perkin Elmer). Libraries were generated from 250 ng of total RNA as following ...
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bioRxiv - Immunology 2023Quote: RNA libraries were generated using the NEXTFLEX® Small RNA-Seq Kit (PerkinElmer, USA; Cat. No.: NOVA-5132-05). For each library ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed using the Chemagic™ Prime Viral DNA/RNA 300 Kit H96 (Chemagic, CMG-1433, PerkinElmer). Detailed concentration and DNA extraction procedures are provided in the Supplementary materials (Materials and Methods ...
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bioRxiv - Immunology 2024Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of this first messenger was determined using the Lance Ultra cAMP kit (Perkin Elmer, Waltham, MA, US). The method consists of a time-resolved fluorescence resonance energy transfer (TR-FRET ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was isolated from a single colony using a JANUS Chemagic Workstation and Chemagic Viral DNA/RNA kit (PerkinElmer). Libraries were prepared with the Nextera XT DNA sample preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity was measured using Pierce™ Firefly Luciferase Glow Assay Kit according to manufacturer’s protocol using an EnVision mulitlabel plate reader (PerkinElmer). AlamarBlue (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Genomics 2022Quote: ... The DNA extraction was performed at Hospital Universitari Vall d’Hebron (Supplementary Table 2) from blood samples using chemagic DNA Blood400 Kit LH (Perkin Elmer). The DNA from oral mucosa swabs ...
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bioRxiv - Molecular Biology 2019Quote: Sequencing libraries were prepared from immunoprecipitated piRNAs using the NEXTflex Small RNA-Seq Kit v3 (PerkinElmer Cat# NOVA-5132-05). The manufacturer’s protocol was followed ...
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bioRxiv - Cell Biology 2019Quote: RNA was isolated from the ZEB1 CEnC lines and RNA-seq libraries were prepared using the KAPA mRNA HyperPrep Kit using an automated liquid handler (Janus G3 – PerkinElmer) according to manufacturer’s instructions at the UCLA Institute for Quantitative and Computational Biology ...
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bioRxiv - Genetics 2021Quote: ... 100 ng of polyA-selected mRNA from each meiotic stage was prepared using the NEXTFLEXTM Rapid Directional RNA-seq Kit (NOVA-5138-08; PerkinElmer) according to the provided manual ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 75] and Illumina sequencing libraries prepared using PerkinElmer NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 (Perkin Elmer, Waltham, MA, USA).The libraries were sequenced using a S4 NovaSeq flow cell with the MiSeq 600-cycle v3 kit ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer). Libraries were pooled in equimolar ratios ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular culture media were collected in order to dose secreted Aβ using Alpha-LISA assays (AlphaLISA Amyloid β1-X Kit, AL288C, PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... All DNA extractions were performed using the QuickPickTM SML Plant DNA Kit (Bio-Nobile, Turku, Finland) as in PM7/24 (EPPO, 2018b) with an automated system (Caliper Zephyr, PerkinElmer). A control composed of DNAs extracted from bacterial suspensions were systematically performed.
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bioRxiv - Cell Biology 2021Quote: ... Prior to sequencing libraries were prepared using NEXTFLEX Rapid DNA-Seq Library Prep Kit for Illumina Platforms (PerkinElmer, Waltham, MA) according to manufacturer’s protocol ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: Phosphorylated SYK (p-Syk) was measured using the AlphaLISA SureFire Ultra p-Syk Assay Kit (PerkinElmer, #ALSU-PSYK-A-HV) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...
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bioRxiv - Cell Biology 2019Quote: RNA was isolated and RNA-seq libraries were prepared using the KAPA mRNA HyperPrep Kit with an automated liquid handler (Janus G3 – PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: The ligand-induced phospho-ERK1/2 signaling was assessed using the AlphaLISA Surefire Ultra p-ERK1/2 (Thr202/Tyr204) kit (PerkinElmer) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: Phospho-Syk level from WT mouse primary microglia cultures were determined using AlphaLISA SureFire Ultra kit (PerkinElmer, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... loads were adjusted and proteins were resolved by SDS-PAGE and analyzed by western-blot (Western Lightning Plus-ECL kit; PerkinElmer). For Flag immunoprecipitations ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed in PBS containing 0.1% Tween-20 and then exposed to developing solution (Western Lighting Chemiluminescence kit, Perkin Elmer) according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For cDNA generation and construction of the sequencing libraries we used the NEXTflex Rapid Illumina Directional RNA-Seq Library Prep Kit (PerkinElmer) followed by paired-end sequencing on an Illumina NextSeq 500 at Viroscan3D (Lyon ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was measured using a Promega Nano-Glo Assay Kit and read on an Envision microplate luminometer (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: ... an equivalent of 500 ng of p24 Gag was lysed in buffer with 1% Triton (ELISA kit, Alliance Perkin Elmer) for 30 min on ice ...
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bioRxiv - Physiology 2020Quote: ... The probes were then detected using Tyramide signal amplification (TSA) reaction using an Alexa-flour labelled TSA kit (PerkinElmer Bioscience) based on the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA extraction from K2-EDTA-blood tubes was performed with chemagic 360 instrument and the CMG-704 kit (PerkinElmer), which uses magnetic bead technology ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Concentration of HIV p24 antigen in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA) and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... The cortisol ELISA assay was performed as per manufacturer’s instructions using the Cisbio HTRF cortisol ELISA kit (62CRTPEG, Cisbio, Perkin Elmer) and measurements were performed on Tecan Infinite M1000 Pro multi-plate reader.
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bioRxiv - Microbiology 2022Quote: ... Quality of the libraries was measured with LabChip GX Touch HT DNA High Sensitivity Reagent Kit (PerkinElmer, Waltham, MA, USA). Sequencing was performed with NovaSeq 6000 System (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: RNA purified from the IPs and ribodepleted RNA from the input samples were processed for library preparation with the Nextflex Small RNA kit v3 (#NOVA-5132-06, PerkinElmer), following manufacturer instructions ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Plant Biology 2024Quote: ... the fragmented gDNA was constructed into sequencing libraries using a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA). The full-length coding sequence of ZmHsf20 was cloned into the pFN19K HaloTag T7 SP6 Flexi vector ...
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bioRxiv - Pathology 2023Quote: ... double immunohistochemistry for paraffin sections (8 μm) was conducted by using the Opal 4 color manual immunohistochemistry (IHC) kit (NEL810001KT, PerkinElmer). The signal of p-MLC2 was amplified by the Opal 4 color manual IHC kit (NEL810001KT ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescent signals corresponding to the target transcript were generated using the Tyramide Signal Amplification (TSA) Plus Fluorescein Kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were prepared from 33ng of genomic DNA using a custom miniaturized version of the NEXTFLEX Rapid XP kit protocol (PerkinElmer) that was automated on a Sciclone NGSx liquid handler (PerkinElmer) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of nascent RNA was used as input to construct plaNETseq libraries using NEXTflex Small RNA-seq kit v3 (PerkinElmer) with a modified protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Free amino acids were determined with the EZ:FAAST amino acid analysis kit of Phenomenex on a GC-FID (Perkin Elmer) according to supplier instructions ...
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Genetics 2023Quote: ... 10 µg of total RNA was polyA-selected and processed using the NEXTFLEX Rapid Direction RNA-seq Kit (NOVA-5138-10 and NOVA-5138-11; PerkinElmer) according to the provided manual ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IP1 levels were determined using the Cisbio HTRF® IP-one assay kit per the manufacturer’s instructions and measured using an Envision plate reader (PerkinElmer). Data are expressed as fold of basal IP1 levels.
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bioRxiv - Immunology 2023Quote: ... The slides were subjected to sequential staining cycles as described before,3 although now automated using Opal 7-color Automation IHC Kit (NEL801001KT; PerkinElmer) on the BOND RX IHC & ISH Research Platform (Leica Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... reagents and conditions of the BigDye Terminator v3.1 Cycle sequencing Kit and an automated capillary sequencer ABI 3700 DNA analyzer (Perkin Elmer, Applied Biosystems ...