Labshake search
Citations for PerkinElmer :
601 - 650 of 679 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested post-stimulation and the production of TNF-α was assessed using the AlphaLISA Immunoassay Kit (PerkinElmer, AL208C) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Media supernatants of the 4T1 cells in the plates were collected and immediately analyzed with ATPlite one-step luminescence assay kits (PerkinElmer, MA) for the measurement of ATP concentration in the media ...
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bioRxiv - Cell Biology 2022Quote: ... Accumulation of IP1 was measured using the IP-One Gq Homogenous Time-Resolved Fluorescence (HTRF) kit from Perkin Elmer (Waltham, MA), per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Biochemistry 2022Quote: ... The homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the Cisbio IP-One Gq kit (Cisbio Bioassays, Perkin Elmer). Dose-response curves were fitted by Prism 8 (Graphpad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following an HRP conjugation step a TSA Opal 570 1:150 (Tyramide Signal Amplification Opal 4-Color Manual IHC Kit, Perkin Elmer) was linked to the IRF7 antibody previously bound to the slide overnight ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Genomics 2023Quote: ... and streptavidin-conjugated Atto-425 (Bio Trend) at a concentration of 1:400 using TSA-biotin (TSA Plus Biotin Kit, Perkin Elmer). All nuclei were DAPI-stained ...
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bioRxiv - Physiology 2024Quote: ... Analysis of plasma glucagon and insulin levels were performed with the corresponding mouse plasma insulin (HTRF insulin mouse serum kit, ref: 62IN3PEF, Cisbio/Perkin Elmer) and glucagon (mouse glucagon ELISA kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were prepared following the NEXTflex Small RNA-Seq Kit v3 protocol provided by Perkin Elmer (Perkin Elmer, Waltham, MA, USA), starting with 200 ng of treated RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... or from 10 µg total RNA from gravid adults (for analysis of protein-coding genes near dysregulated piRNAs on Chromosome IV) using the stranded NEXTFLEX Rapid Directional RNA-seq kit (Perkin Elmer). The embryonic and larval libraries represented two independent biological replicates of wild type and ebax-1(tm2321 ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenization buffer was prepared as a mix of 10% ‘Lysis & Detection Buffer’ and 90% ‘Stimulation Buffer’ 1× from the IP-One Gq kit (Perkin Elmer). While the exact formula of this buffers is proprietary ...
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bioRxiv - Bioengineering 2024Quote: ... These cells enabled calibration of a glucagon-dependent cAMP production assay using the LANCE™ Ultra cAMP Kit (PerkinElmer, Waltham, MA) following manufacturer instructions.
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bioRxiv - Cancer Biology 2024Quote: ... total RNA samples were subjected to Poly(A) selection and purification using the NEXTFLEX Poly(A) Beads Kit 2.0 following the manufacturer’s protocol (Perkin Elmer Applied Genomics). Pooled mRNA sequencing libraries were sequenced on an Illumina NovaSeq S4 at the UC Davis Sequencing Core Facility ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of total RNA for each sample was used for mRNA library preparation using the NEXT-FLEX Rapid Directional RNA-Seq Kit 2.0 following the manufacturer’s protocol (Perkin Elmer Applied Genomics). Before library preparation ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was washed in TTBS for 3 times and the signal was developed using Western Lightening Plus-ECL kit (PerkinElmer, Waltham, MA) and detected on HyBlot CL X-ray films (Denville).
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bioRxiv - Neuroscience 2020Quote: ... Double immunofluorescence was performed using tetramethyl rhodamine labelled secondary antibody for alpha-synuclein and NSF with the fluorescein signal amplification kit (Perkin Elmer, UK). Following adequate washes to remove background fluorescence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Detection of either ERK1/2 or Akt1/2/3 phosphorylation was performed as described in the corresponding AlphaScreen SureFire kits and fluorescence measured with an EnVision plate reader (PerkinElmer, Boston, MA). Data were normalised to the response elicited upon stimulation of cells with 10% FBS.
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bioRxiv - Microbiology 2021Quote: ... 1-3ng/μl of the PCR amplifier of the HPV L1 gene was mixed with 8 μL of ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit version 1.1(Perkin Elmer Biosystems, USA), 2 pmol of primer ...
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bioRxiv - Microbiology 2021Quote: ... using 100 ng total RNA for library preparation with the NEXTFLEX® Small RNA-Seq Kit v3 (PerkinElmer Inc., Waltham, MA, USA), followed by library sequencing on one lane NovaSeq6000 SP v1.0 (2×50bp) ...
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bioRxiv - Physiology 2020Quote: Renal tissue cAMP production following ligand stimulation was measured using the time-resolved fluorescence energy transfer (TR-FRET) based LANCE ULTRA cAMP Kit (PerkinElmer, MA, USA) in combination with an EnSight Multimode Plate Reader (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: ... 75 µL of supernatant from each well was collected for ATP supernatant detection using the ATPlite Luminescence Assay Kit (Perkin Elmer, 6016943) as described by the kit protocol ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest for 48 h and luciferase activity was measured with Promega Steady-Glo® kit (E2550) using a Victor X3 Multilabel Reader (Perkin Elmer). Each experiment shown is representative of three independent experiments.
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from 250 mg of leaves ground to powder under liquid nitrogen using the Chemagic DNA Plant Kit (Perkin Elmer, Germany), according to the manufacturer’s instructions on a KingFisher Flex™ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cAMP levels were measured with an Amplified Luminescent Proximity Homogeneous Assay (ALPHA) Screen cAMP assay kit as instructed by the manufacturer (PerkinElmer, Waltham, MA).
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An updated C. elegans nuclear body muscle transcriptome for studies in muscle formation and functionbioRxiv - Genetics 2023Quote: ... The libraries were prepared from the ribosomal RNA depleted samples using the Nextflex Rapid Directional RNAseq Kit (PerkinElmer Catalog # NOVIA-5138-08) as per the manufacturer’s protocol and sequenced on the Nextseq 500 instrument (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood samples or buffy coat extracts were extracted in Auria Biobank for all patients for DNA extraction and genomic sequencing to serve as germline reference for identifying somatic genomic aberrations using Chemagic DNA Blood Kit Special (PerkinElmer Inc., USA) and Chemagic 360 instrument (PerkinElmer Inc. ...
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bioRxiv - Immunology 2024Quote: ... 1:1 mixed with maleate buffer (R2a from the kit)) was added and incubated at room temperature and measured kinetically on an Envision microplate reader (PerkinElmer, Waltham, MA) at 340 nm for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... These purified ribosome footprints were used as input for the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06). The resulting cDNA was amplified by 11-16 cycles of PCR with a barcode incorporated in the primer ...
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bioRxiv - Physiology 2023Quote: ... and blood was collected for the measurement of both glucose (Accu-Check) and insulin levels (Insulin AlphaLISA detection kit, Perkin Elmer, USA).
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bioRxiv - Physiology 2023Quote: ... Perfusates were continuously and automatically collected into 96-well plates for the detection of insulin (Insulin AlphaLISA Detection Kit, Perkin Elmer, USA). Insulin measurement was performed according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Microbiology 2024Quote: ... June 2022) The libraries were quantified on the Perkin Elmer LabChip GX Touch with the DNA High Sensitivity Reagent kit (Perkin Elmer, CLS760672). Libraries were pooled in equimolar ratios ...
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bioRxiv - Genomics 2024Quote: ... Stranded poly(a) selected libraries were then prepared with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer Inc., Waltham, MA). For both species ...
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bioRxiv - Biochemistry 2024Quote: The levels of Aβ42 in brain samples or in neuron culture media were determined by amplified luminescent proximity homogeneous assay (AlphaLISA) using AL203 C/F kit (PerkinElmer, Waltham, USA) from PerkinElmer ...
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bioRxiv - Developmental Biology 2024Quote: ... Transcripts were fluorescently labeled with Opal 520 (1:1500) and Opal 570 (1:500) using the Opal 7 Kit (Perkin Elmer; NEL797001KT). Images were acquired on a Zeiss LSM 880 inverted confocal system with 20X air and 40X oil objectives using Zen Black software (Carl Zeiss) ...
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bioRxiv - Developmental Biology 2024Quote: ... phosphor-Shc and phospho-Akt staining was amplified using a Tyramide Signal Amplification kit (TSATM Plus System, PerkinElmer Life Sciences, Waltham, MA). Alexa Fluor secondary antibodies and Alexa Fluor 488 phalloidin (A-12379 ...
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bioRxiv - Cell Biology 2020Quote: LH levels in the medium were measured by means of a time-resolved fluorometric immunoassay with rat LH assay kits (National Hormone and Peptide Program, NHPP) and the Delfia system (Perkin Elmer, Waltham, MA) as reported previously (Kawaminami et al. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP) conjugates with Cyanine 5 Plus Amplification Reagent in a TSA kit as the substrate (PerkinElmer, Boston, MA, USA). Sense probes labelled with DIG and biotin by SP6 RNA transcription kit were used as control ...
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bioRxiv - Cell Biology 2021Quote: ... the RBP-RNA complex was purified and the bound RNA eluted for subsequent Illumina library preparation (NEXTFLEX small RNA library preparation kit v3, PerkinElmer #NOVA-5132-06) and paired-end (40×43 cycles ...
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bioRxiv - Neuroscience 2020Quote: ... Cux2 (Atlas Antibodies, Stockholm, Sweden; rabbit, 1:200; detected with ABC kit [Vector Laboratories, Burlingame, CA, USA] and TSA [PerkinElmer, Waltham, MA, USA]), Dcx (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2020Quote: The amounts of cAMP in the cytosol were measured by using the time-resolved fluorescence resonance energy transfer (TR-FRET) kit from Perkin Elmer (EnVision Xcite), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Immunology 2020Quote: ... The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip GX system (PerkinElmer, Waltham, MA, USA). The libraries were qPCR quantified (Kapa Biosystems ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays, Perkin Elmer, Codolet, France) according to the manufacturer’s recommendations (112) ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular ATP and extracellular lactate levels were measured for each NCC primary cultures after Seahorse analysis using an ATPlite Bioluminescence assay kit from PerkinElmer (cat. no. 6016943) and the Lactate Fluorometric Assay kit from BioVision (cat ...
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bioRxiv - Physiology 2020Quote: ... 1 ng of eluted and purified DNA was used to prepare DNA sequencing library with the Nextflex rapid DNA seq kit 2.0 (Perkin Elmer, NOVA-5188-01) on the NextSeq 500 system (Illumina ...