Labshake search
Citations for PerkinElmer :
551 - 600 of 679 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Prior to sequencing libraries were prepared using NEXTFLEX Rapid DNA-Seq Library Prep Kit for Illumina Platforms (PerkinElmer, Waltham, MA) according to manufacturer’s protocol ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: Phosphorylated SYK (p-Syk) was measured using the AlphaLISA SureFire Ultra p-Syk Assay Kit (PerkinElmer, #ALSU-PSYK-A-HV) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...
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bioRxiv - Systems Biology 2020Quote: ... the DIG-labeled probe was visualized using a tyramide-Cy3 Plus kit (1:50 dilution, 15-minute incubation, Perkin Elmer). Following washes in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: Phospho-Syk level from WT mouse primary microglia cultures were determined using AlphaLISA SureFire Ultra kit (PerkinElmer, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was performed using the either the TSA Cyanine 3 Plus or the TSA Plus Fluorescein Evaluation Kits (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed in PBS containing 0.1% Tween-20 and then exposed to developing solution (Western Lighting Chemiluminescence kit, Perkin Elmer) according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For cDNA generation and construction of the sequencing libraries we used the NEXTflex Rapid Illumina Directional RNA-Seq Library Prep Kit (PerkinElmer) followed by paired-end sequencing on an Illumina NextSeq 500 at Viroscan3D (Lyon ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was measured using a Promega Nano-Glo Assay Kit and read on an Envision microplate luminometer (Perkin Elmer).
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bioRxiv - Physiology 2020Quote: ... The probes were then detected using Tyramide signal amplification (TSA) reaction using an Alexa-flour labelled TSA kit (PerkinElmer Bioscience) based on the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA extraction from K2-EDTA-blood tubes was performed with chemagic 360 instrument and the CMG-704 kit (PerkinElmer), which uses magnetic bead technology ...
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bioRxiv - Systems Biology 2023Quote: ... Free amino acids were determined with the EZ:FAAST amino acid analysis kit of Phenomenex on a GC-FID (Perkin Elmer) according to supplier instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... reagents and conditions of the BigDye Terminator v3.1 Cycle sequencing Kit and an automated capillary sequencer ABI 3700 DNA analyzer (Perkin Elmer, Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... The slides were subjected to sequential staining cycles as described before,3 although now automated using Opal 7-color Automation IHC Kit (NEL801001KT; PerkinElmer) on the BOND RX IHC & ISH Research Platform (Leica Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IP1 levels were determined using the Cisbio HTRF® IP-one assay kit per the manufacturer’s instructions and measured using an Envision plate reader (PerkinElmer). Data are expressed as fold of basal IP1 levels.
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bioRxiv - Genetics 2023Quote: ... 10 µg of total RNA was polyA-selected and processed using the NEXTFLEX Rapid Direction RNA-seq Kit (NOVA-5138-10 and NOVA-5138-11; PerkinElmer) according to the provided manual ...
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bioRxiv - Microbiology 2022Quote: ... Quality of the libraries was measured with LabChip GX Touch HT DNA High Sensitivity Reagent Kit (PerkinElmer, Waltham, MA, USA). Sequencing was performed with NovaSeq 6000 System (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: RNA purified from the IPs and ribodepleted RNA from the input samples were processed for library preparation with the Nextflex Small RNA kit v3 (#NOVA-5132-06, PerkinElmer), following manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescent signals corresponding to the target transcript were generated using the Tyramide Signal Amplification (TSA) Plus Fluorescein Kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of nascent RNA was used as input to construct plaNETseq libraries using NEXTflex Small RNA-seq kit v3 (PerkinElmer) with a modified protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer, Austin, Texas, USA). Quality of library preparation was assessed on a 2100 Agilent Bioanalyzer ...
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bioRxiv - Evolutionary Biology 2023Quote: Libraries were prepared from 33ng of genomic DNA using a custom miniaturized version of the NEXTFLEX Rapid XP kit protocol (PerkinElmer) that was automated on a Sciclone NGSx liquid handler (PerkinElmer) ...
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bioRxiv - Plant Biology 2023Quote: ... RPFs were directly used as input into the NEXTflex small RNA-seq kit v3 (Perkin Elmer, cat# NOVA-5132-06) or V4 (Perkin Elmer ...
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bioRxiv - Plant Biology 2024Quote: ... the fragmented gDNA was constructed into sequencing libraries using a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA). The full-length coding sequence of ZmHsf20 was cloned into the pFN19K HaloTag T7 SP6 Flexi vector ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Concentration of HIV p24 antigen in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA) and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Amplified Luminescent Proximity Homogeneous Assay Screen (ALPHAScreen) was carried out utilising the cMyc detection kit and Proxiplate-384 Plus plates (PerkinElmer) as previously described (Martin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µ g of lysate was used for the assessment of ATP concentration using an ATP Determination Kit (ATP lite assay, PerkinElmer) as described earlier (Miyoshi et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and an aliquot from the supernatant was collected to quantify cAMP using the LANCE cAMP kit (Perkin Elmer, cat#AD0262) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... using a HyBEZ® oven Probes were detected using Tyramide signal amplification (TSA) reaction (1:600 dilution) using an Alexa-flour labeled TSA kit (PerkinElmer) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Library preparation for miRNA-sequencing was performed using the NEXFLEX small RNA-seq kit v3 (Perkin Elmer, Waltham, MA, USA). Small-RNA libraries were sequenced on an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... involving the use of a biotinylated secondary antibody followed by an ABC complex and Cy3-tyramide kit (Perkin Elmer, cat#: FP1046) or an Alexa secondary antibody (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... All treated and RNA-extracted samples were then used to produce small libraries for sequencing using a NETFLEX small RNA-Seq kit (PerkinElmer, USA).
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bioRxiv - Plant Biology 2020Quote: ... Final library products were assessed for quality on LabChip GX using the DNA High Sensitivity Reagent Kit (Perkin Elmer, MA, USA), diluted to 2 nM concentration and pooled ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin concentrations (from tail vein samplings) were determined using an ultrasensitive mouse insulin AlphaLisa detection kit (cat number AL204C) from Perkin Elmer.
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bioRxiv - Microbiology 2020Quote: ... Stool samples underwent DNA extraction using chemical and mechanical lysis with magnetic bead-based purification via the Chemagic MSM I with the Chemagic DNA Blood Kit-96 from Perkin Elmer. Prior to extraction on the MSM-I ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA from parents and progenies was extracted from 100 mg of frozen young leaves with the Chemagic DNA Plant Kit (Perkin Elmer Chemagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Ligand-induced ERK1/2 phosphorylation was assessed using the AlphaLISA Surefire Ultra p-ERK1/2 (Thr202/Tyr204) kit (PerkinElmer, Melbourne, Australia) following the manufacturer’s protocol as previously described (23 ...
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bioRxiv - Genomics 2021Quote: ... the anti-DIG-HRP antibody was detected with the TSA-Cy3 substrate (1:50, TSATM PLUS Cy3 kit, NEL 745001KT, Perkin Elmer) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA concentration was measured with Quant-iT™ PicoGreen™ dsDNA Assay Kit after it was eluted in 400µl 10mM Tris-EDTA elution buffer (PerkinElmer). Aliquotes of DNA samples were produced with Tecan Genesis/Tecan Freedom Evo and then shipped on dry ice for genetic analyses ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were generated from a mixture of poly(A)+ enriched and rRNA-depleted samples using the NEXTflex® Rapid Directional qRNA-Seq Kit (PerkinElmer). Fragmentation of the RNAs was carried out enzymatically at 95°C for 10 min via the addition of NEXTflex® RNA Fragmentation Buffer ...
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bioRxiv - Pathology 2023Quote: ... as previously described [71] using Multiplex Fluorescent Reagent Kit V2 (Advanced cell Diagnostics) and TSA Plus Cyanine 3 and Cyanine 5 (Perkin Elmer) as fluorophores according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were cut as 4 μm sections and automated IF was performed on a Leica Bond Rx Platform at room temperature using the PerkinElmer Opal 4-Colour Automation IHC Kit (PerkinElmer, #NEL800001KT). Tissue sections were blocked with 3% Hydrogen peroxide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: The AlphaLISA pSTAT1 assay was performed using the AlphaLISA SureFire Ultra p-STAT1 (Tyr701) assay kit (PerkinElmer, Inc., Waltham, MA, USA). HeLa cells (CCL-2 ...