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Citations for CisBio :
251 - 300 of 313 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells were labelled with SNAP-Lumi4-Tb (Cisbio, 40 nM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... onto the C-terminus end of the SNAP-GLP-1R vector (Cisbio), followed by site-directed mutagenesis of the GLP-1R stop codon ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... generated by stable transfection of pSNAP-GLP-1R (Cisbio) into HEK293 cells [20] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A sample of supernatant was removed and analysed for insulin content by HTRF (Wide Range Insulin HTRF Kit, Cisbio). Results were expressed relative to insulin released with 11 mM glucose alone.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Immunology 2021Quote: A homogeneous time-resolved fluorescence (hTRF) assay [26] comprised of terbium-labeled anti-human IgG (H+L) (Cisbio, custom label) donor and AF488-labeled anti-HIS (Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... TR-FRET assays on stable doxycycline-inducible NIH 3T3 Tet-on 3G cells expressing Gpr161 or Gpr161mut1 were performed according to manufacturer’s instructions (Cisbio) (Mukhopadhyay et al. ...
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bioRxiv - Developmental Biology 2021Quote: We used a TR-FRET cAMP assay (Cisbio) for estimating cAMP as these assays eliminate the background noise due to FRET-based estimation of signals ...
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bioRxiv - Cell Biology 2021Quote: ... 16.7 mM glucose and 45mM KCl treatments were collected and insulin amount determined using a human insulin HTRF assay (Cisbio). Cells infected with lentivirus were incubated for 6 days prior to GSIS assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Cell Biology 2020Quote: Intracellular cAMP and IP1 was determined by HTRF (cAMP Dynamic 2 and IP-one, respectively (CisBio)) ...
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bioRxiv - Cell Biology 2020Quote: ... IP1 concentrations were measured from cell lysates using the HTRF IP-One immunoassay kit (CisBio). Fluorescence was measured and IP1 levels were quantified using the same methodology as the cAMP assay.
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG; phospho-Yap [Ser127] Cellular Kit, Cisbio 64YapPEG). The signal was detected by Synergy NEO (Biotek).
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bioRxiv - Developmental Biology 2020Quote: The in vitro kinase assay (HTRF KinEASE-STK S1, CisBio 62ST1PEB) was optimized to the linear reaction range of the enzymes Lats1 (Carna 01 −123 ...
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bioRxiv - Molecular Biology 2020Quote: ... in labelling buffer (CisBio) for 2 hrs at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... IP1 accumulation was measured using the HTRF IP-One kit (Cisbio) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Secreted insulin in the supernatant was analysed by HTRF (Insulin High Range kit, Cisbio) after dilution and normalised to the concentration in glucose-only treated wells.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HTRF detection reagents (cAMP Dynamic 2 kit, Cisbio) were added at the end of the incubation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... generated by stable transfection of pSNAP-GLP-1R (Cisbio), previously described in (50) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were obtained from Cisbio. PathHunter detection reagents were obtained from DiscoverX ...
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bioRxiv - Biochemistry 2020Quote: The Tag-lite labelling medium (Labmed) and SNAP-Lumi4-Tb were obtained from Cisbio (Codolet, France). Components for solubilisation buffer and ligands for both ThermoFRET stability assay and fluorescent ligand binding experiments were obtained from Sigma-Aldrich (Havervill ...
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bioRxiv - Biochemistry 2020Quote: V2R binding studies using TagLite® assays (Cisbio, Codolet, France) based on time-resolved-FRET measurements were previously described70,71 ...
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bioRxiv - Neuroscience 2020Quote: ... subsequently lysed in cell lysis buffer from the IP-One-Gq kit (Cisbio Bioassays, Codolet, France) and frozen to -20°C ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of fluorophore-conjugated antibodies was adjusted following the recommendation from the manufacturer (Cisbio). Experiments were performed in 384 well plates with a final volume of 20 μl per well ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Tag-lite SNAP Lumi4-Tb was purchased from Cisbio (Codolet, France). DAMGO was purchased from Abcam (Cambridge ...
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bioRxiv - Biochemistry 2020Quote: ... The cell content was collected and used for IP1 assay (the homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the manufacturer’s directions (Cisbio Bioassays, Perkin Elmer). In brief ...
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Light effects on circadian and homeostatic regulation: alertness increases independent of time awakebioRxiv - Neuroscience 2020Quote: ... Cortisol concentrations were detected with RIA analysis (CORT-C2; Cisbio, Bioassays ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... + 2.5% cAMP-d2 conjugate (Cisbio). The plate was incubated in the dark for 60 min at room temperature (∼22 °C ...
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bioRxiv - Physiology 2020Quote: ... or using a Homogeneous Time Resolved Fluorescence (HTRF) insulin kit (Cisbio, France) in a PHERAstar reader (BMG Labtech ...
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bioRxiv - Physiology 2020Quote: ... Insulin concentration (ng/ml) was measured using a HTRF Insulin Ultra-Sensitive Assay kit (Cisbio, France).
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bioRxiv - Molecular Biology 2020Quote: ... and VASP pSer239 (cat#63ADK065PEG, cat#63ADK067PEG; CisBio) and the phosphorylation signals were normalized to the total level of SMAD3 or VASP ...
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bioRxiv - Molecular Biology 2020Quote: The treated HFL lung fibroblasts were lysed for analysis of SMAD3 pSer423/425 (cat#63ADK025PEH, cat#64ND3PEH; CisBio) and VASP pSer239 (cat#63ADK065PEG ...
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bioRxiv - Cell Biology 2020Quote: ... The secretion medium were then collected to measure the insulin and proinsulin concentrations using respectively an insulin HTRF kit (Cisbio Bioassays) and a rat/mouse proinsulin ELISA kit (Mercodia).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The IP-One ELISA assay kit from CisBio Bioassays ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... performed with the IP-One terbium immunoassay kits from Cisbio, was used according to the manufacturer recommendation and as previsouly described (43) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and Tag-lite SNAP-Lumi4-Tb from Cisbio Bioassays (Codolet ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... stably expressing human SNAP-GLP-1R (“MIN6B1-SNAP-GLP-1R”)(15) were generated by transfection of a SNAP-GLP-1R vector (Cisbio) followed by G418 selection and maintained in DMEM with 15% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... medium was removed and cells were incubated with 50 nM SNAP-Lumi4-Tb (CisBio) in labelling buffer (CisBio ...
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bioRxiv - Neuroscience 2020Quote: ... a homogeneous time-resolved fluorescence (HTRF) immunoassay was used for LRRK2 and pS935-LRRK2 according to manufacturer’s guidelines (Cisbio). Recombinant full-length human G2019S-LRRK2 protein (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytosol cAMP was measured using a cell-based homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) method (Cisbio Bioassays) (19,20) ...
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bioRxiv - Biochemistry 2020Quote: ... Sch-442,416-Red (F-Sch-442,416) was obtained from Cisbio. Microtiter 384-well Proxiplates for the ThermoFRET stability assay and 384-well Optiplates for association binding experiments were obtained from PerkinElmer (Beaconsfield ...
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bioRxiv - Neuroscience 2020Quote: ... After incubation for 1 hour at 37°C the IP1 accumulation was stopped by addition of 40 μL CisBio IP-One Tb HTRF Kit (CisBio, 62IPAPEC) lysis buffer ...
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bioRxiv - Physiology 2020Quote: Receptor-mediated intracellular changes in IP1 second messenger were measured using IP-one- Gq kit (Cisbio) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... IP-1 ELISA (Cisbio) assay was then used according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Supernatant insulin concentration was determined by HTRF (High Range Insulin kit, Cisbio). Results were normalised to those obtained with 11 mM glucose but no additional agonist ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ERK1/2 phosphorylation was determined by HTRF (Cisbio Phospho-ERK [Thr202/Tyr204] cellular kit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cAMP was quantified by HTRF (cAMP Dynamic 2, Cisbio), and responses were normalised to that of forskolin ...