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Citations for CisBio :
1 - 50 of 313 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... following manufacturer’s instructions (62MIL1BPEG, CisBio). Briefly ...
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bioRxiv - Pathology 2024Quote: cAMP-Gs HiRange standard (Cisbio, 62AM6CDA) was dissolved in 11.2 mM 3-isobutyl-1methylxanthine (IBMX ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: FPR-mediated inhibition of cellular de novo cAMP formation was quantified by competitive immunoassays based on the time-resolved measurement of fluorescence resonance energy transfer (HTRF, Homogeneous Time-Resolved Fluorescence) using the cAMP-Gi Kit from Cisbio as previously published [37] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ERK1/2 phosphorylation in HEK293 FPR cell systems was measured with the HTRF immunoassay from Cisbio, as described previously [37] ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenates were diluted 1:50 and InsP levels were assessed with the IP-One ELISA kit (Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Adherent cells were labelled with 100nM SNAP-Lumi4-Tb labelling reagent in Labmed buffer (Cisbio, UK) for 1 hr at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: The competitive cortisol assay (Cisbio HTRF® Cortisol Kit, 62CRTPEG) was performed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Secreted insulin was detected using a commercially available insulin detection kit (PerkinElmer/Cisbio Bioassays, Bedford, MA) based on homogenous time-resolved fluorescence resonance energy transfer (HTRF ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Tag-lite SNAP-Lumi4-Tb labelling reagent and LABMED buffer was purchased from Cisbio (Codolet, France). Polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with the anti-βarr2-Tb antibody (Revvity Cisbio) or anti-AP2-Tb antibody (Revvity Cisbio ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Biochemistry 2024Quote: ... The 5 µl of reaction mixture was transformed to HTRF 96 well low volume plates (Cisbio) and the consumption of ATP was measured with ADP-Glo Kinase Assay kit (Promega Corporation ...
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bioRxiv - Cancer Biology 2024Quote: Chk1 enzyme activity was measured using an HTRF KinEASE assay (Cisbio, catalog no. 62ST1PEC). Full-length human CHK1 protein (GenBank accession number NP_001265.1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was stopped by addition of detection buffer (Cisbio, 10 µL) containing (final concentrations) ...
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bioRxiv - Cancer Biology 2024Quote: ... HTRF signal was read on an EnVision multimode plate reader (CisBio) in HTRF mode ...
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bioRxiv - Cell Biology 2024Quote: βarr1 and βarr2 expression in cells was measured using a total βarr1 and βarr2 cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... Transfected cells in black non-transparent 96-well plates were labeled in Tag-lite labeling buffer (Revvity Cisbio) with 100 nM SNAP-Lumi4-Tb for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... After three times washing with wash buffer (Revvity Cisbio), cells were incubated with the anti-βarr2-Tb antibody (Revvity Cisbio ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were fixed with stabilization buffer (Revvity Cisbio) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: βarr2/AP2 interaction in cells was measured using a β-arrestin2/AP2 interaction (62BDBAR2PEB) cellular kit (Revvity Cisbio), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... or anti-AP2-Tb antibody (Revvity Cisbio) at final concentration of 0.5 nM for 2 h ...
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bioRxiv - Biophysics 2024Quote: ... Labeling with the Tb-cryptate for the LRET experiments was carried out by incubating the β-arrestin 1 with 2 equivalents of Lumi-4 Tb maleimide (CisBio) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... INS-1 832/3 SNAP/FLAG-hGLP-1R WT and V229A cells were generated in house from INS-1 832/3 GLP-1R KO cells following transfection with pSNAP/FLAG-hGLP-1R (Cisbio) or pSNAP/FLAG-hGLP-1R-V229A (generated in house by site-directed mutagenesis ...
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bioRxiv - Cell Biology 2024Quote: Insulin concentrations from cells and islets were determined using the Insulin Ultra-Sensitive HTRF Assay Kit (2IN2PEG, Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were labelled with 40 nM SNAP-Lumi4-Tb (SSNPTBC, Cisbio), a lanthanide fluorophore probe for TR-FRET ...
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bioRxiv - Cell Biology 2024Quote: Human SNAP/FLAG-tagged GLP-1R (SNAP/FLAG-hGLP-1R) (Cisbio) was used as the template to generate GLP-1R cholesterol binding mutants for screening ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Anti-Flag d2 conjugated antibody was purchased from Cisbio Bioassays (Cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and anti-Flag-d2 (1:500 dilution, Cisbio Bioassays Cat. 61FG2DLF), were added at 0.2 μL per well by robotic dispenser ...
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bioRxiv - Biochemistry 2024Quote: ... Further characterization of nanobody activity at the hmGlu5 receptor was performed 24 h post transfection using the inositol monophosphate (IP1) accumulation assay (IpOne assay; Cisbio) as described below.
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bioRxiv - Biophysics 2024Quote: ... 2 nM MAb Anti 6HIS-Tb cryptate HTRF reagent (Cisbio), 25 nM Streptavidin Alexa Fluor™ 488 conjugate (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Phosphorylation of ERK1/2 at T202 and Y204 was measured with the HTRF® Advanced Phospho-ERK1/2 assay (Cisbio Bioassays, Codolet, France). The cells were handled gently throughout the procedure to avoid agonist-independent ERK1/2 phosphorylation ...
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bioRxiv - Biochemistry 2024Quote: Inositol monophosphate (IP1) accumulation was measured using the commercially available HTRF® IP-One accumulation kit (Cisbio Bioassays, Codolet, France) and an EnVision multimode plate reader (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: Gq-dependent activation of phospholipase C was quantified by measuring IP1 using the IP-One assay kit (Cisbio, France) as previously described19 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cellular IP1 as a readout for Gq signaling was measured using a commercial HTRF competitive immunoassay (IP-One Gq kit, CisBio) as described in the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HTRF assay kit 64ACCPEG from CisBio was used to quantify endogenous levels of phospho-ACC-Ser79 according to manufacturer’s instructions with the following modifications ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plate was incubated at 37°C for 1 h before addition to each well of 3 µl IP1-d2 and anti-IP1-d2 dissolved in lysis buffer (CisBio HTRF IP-One Gq Kit) and then incubated further at RT for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... GTP binding assay was performed using a GTP Gi Binding Assay Kit (Cisbio) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Anti-His terbium conjugated antibody is a mouse monoclonal antibody (Anti 6His-Tb cryptate, Cat. No. 61HISTLF, Cisbio Bioassays). Anti-Flag d2 conjugated antibody was purchased from Cisbio Bioassays (Cat ...
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bioRxiv - Physiology 2024Quote: ... Analysis of plasma glucagon and insulin levels were performed with the corresponding mouse plasma insulin (HTRF insulin mouse serum kit, ref: 62IN3PEF, Cisbio/Perkin Elmer) and glucagon (mouse glucagon ELISA kit ...
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bioRxiv - Molecular Biology 2024Quote: INS-1 832/3 pancreatic β-cells stably expressing SNAP/FLAG-hGLP1R or SNAP/FLAG-hGIPR were pre-incubated in suspension with 40 nM of SNAP-Lumi4-Tb (SSNPTBC, Cisbio), a lanthanide fluorophore probe for TR-FRET ...
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bioRxiv - Cell Biology 2024Quote: Insulin concentration (ng/ml or pM) was measured using rat insulin ultra-sensitive ELISA kit (Cat#62IN2PEG, Cisbio, Cambridge, England) or human insulin ELISA kit (Cat#90095 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... medium was removed and 10 ml of Tag-lite labeling medium (LABMED, Cisbio) containing 100 nM of SNAP-Lumi4-Tb was added and incubated for 1h at 37°C under 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HRTF assays were performed using the homogenous time-resolved fluorescence (HTRF) HiRange cAMP detection kit (Cisbio, Bedford, MA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:40 in assay diluent 5 and then IL-1β levels were measured using the Human IL- 1β HTRF kit (Cisbio – PerkinElmer), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... supernatants were collected and IL-1β levels were measured using the Human IL-1β HTRF kit (Cisbio – PerkinElmer), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Accumulation of IP1 in UM cells was measured using the IP-One kit (CiSbio, Inc; catalog number 62IPAPEB) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Intracellular cAMP accumulation was measured using the Homogeneous Time-Resolved Fluorescence (HTRF) kit (Cisbio) according to the manufacture’s introduction ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with cAMP-D2 and anti-cAMP Crypate (Cisbio) for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... To an HTRF 96-well low volume white plate (CisBio), 2 µL of (5X ...
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bioRxiv - Cancer Biology 2024Quote: A homogeneous time-resolved fluorescence (HTRF) for cAMP from CisBio (Bedford, MA) was used following the manufacturer’s suggested protocol ...