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Citations for Worthington Biochemical :
301 - 350 of 376 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... muscles were chopped in PBS on ice and digested using 800 U/mL collagenase type 2 (Worthington, Cat# LS004177) in F10 medium (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Samples were transferred to tubes with 2 mL inhibitor solution (3.75 mg/mL ovomucoid, 3.75 mg/mL albumin, 100 U/mL DNaseI, Worthington LK003150) and spun for 5 minutes at 200 g ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... fresh lung tissue was minced into 0.1 cm pieces in petri dishes and treated with 2 mg/mL of collagenase D (Worthington) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The SNc regions were dissected out from each slice and incubated for 20–30 min at 36–37℃ in oxygenated high-glucose HEPES-buffered saline containing 4–8 U/ml papain (Worthington Biochemical Corp., USA, LK0003176). After enzymatic digestion ...
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bioRxiv - Neuroscience 2024Quote: ... ∼20 hCOs were chopped into small pieces using a #10 blade and treated with 30 U/ml Papain (Worthington Biochemical, CAS: 9001-73-4) for 60-90 minutes at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Immunology 2021Quote: ... tissue disaggregation was performed by finely chopping liver samples (~1-2 g) in 10 ml digestion solution containing 1 mg/ml Collagenase IV (Worthington) and 20 μg/ml DNAse1 (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... coli was removed by mixing TNKS1 construct with 2 µM snake venom phosphodiesterase I from Crotalus adamanteus (Worthington Biochemical Corporation).
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bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Immunology 2022Quote: Lung tissue was minced and digested in a RPMI 1640 solution with Type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Surgical samples were minced then digested for 1 hour at 37 °C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The tumor-bearing left lungs were mechanically dissociated using a razor blade and incubated for 30 minutes at 37°C with 3.2mg/mL Collagenase Type 2 (Worthington, 43C14117B), 0.75 mg/ml Elastase (Worthington ...
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bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Immunology 2023Quote: ... A 2 cm2 patch of skin was minced and incubated in 3 mg/mL collagenase Type IV (Worthington Biochemical Corporation) and 2 mg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and the sensory neurons were dissociated by mechanical agitation after incubation for 2 h with collagenase type III (CLS3, Worthington) at 37°C ...
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bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Immunology 2023Quote: ... whole lung was minced with a sharp razor blade and digested with collagenase type IV 2 mg/ml (Worthington, LS004189) and deoxyribonuclease II 4 μg/ml (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... Each 25 μL piece of vitreous was placed in an 8 mm diameter coverslip-bottom chamber with 200 μL of either 2 mg/mL bacterial collagenase type II (Worthington) or phosphate-buffered saline ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro degradation of lignin composites was determined by submerging lignin composites in the solution of collagenase type II (0.5 U/mL, cat# CLS-2, Worthington Biochemical) with 1 mM CaCl2 in serum-free culture medium ...
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bioRxiv - Immunology 2024Quote: ... The cardiac tissues were minced and subjected to repetitive rounds of enzymatic digestion with collagenase type 2 (Worthington Biochemical Corp.) until complete dissociation ...
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bioRxiv - Cell Biology 2024Quote: ... the brain tissues were washed with DM 1∼2 times and incubated with 13mL of DM containing papain (Worthington, LK003176) in 37°C water bath for 20 min ...
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bioRxiv - Immunology 2024Quote: Lung tissue was minced and digested in an RPMI 1640 solution with type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (MilliporeSigma ...
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bioRxiv - Neuroscience 2024Quote: ... samples were pre-cut with small scissors and diluted and digested in 2:3 stock solution of CollagenaseI (10 U/ml, Worthington), Collagenase IV (400 U/ml ...
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bioRxiv - Microbiology 2024Quote: ... D6/2-rotavirus was propagated in MA104 cells in serum-free DMEM supplemented with 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). Harvested cells were then subjected to three freeze/thaw cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... Surgical samples were minced then digested for 1 h at 37°C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were minced and digested using a freshly prepared enzymatic mix containing Collagenase type 2 (2.5 mg/ml; Worthington, LS004174), DNase I (125 U/ml ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... A 5 to 7-day-old protonemal tissue culture was scraped from solid plates and added to a 466 mM mannitol solution with 2% Driselase and Cellulase (21 unit/mL, Worthington CEL). The mixture was gently agitated for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Cell Biology 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Unlabeled and Alexa Fluor 488 (AF488)-labeled T3DCD or T1L virions (2×1012 particles/ml) were digested with 200 μg/ml Nα-p-tosyl-L-lysine chloromethyl ketone-treated chymotrypsin (Worthington Biochemical) in a total volume of 100 μl for 20 min at 32°C [34] ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... with 2 mL pre-warmed digestion enzyme in ACSF solution (Papain, 25 unit/mL and DNase I, 55 unit/mL, Worthington Biochemical). For spinal dorsal horn neuronal atlas construction ...
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bioRxiv - Cell Biology 2024Quote: ... whole hearts from 28 wk-old ♂ WT and R14Δ/+ mice were cannulated and perfused with modified Krebs buffer containing 2 mg/ml collagenase (Worthington) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... and hearts were perfused retrogradely for 15-18 min at 37°C with HEPES-buffer containing 100 mg/mL collagenase type 2 (Cat#9001-12-1, Worthington, USA) and digested ...
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bioRxiv - Cancer Biology 2024Quote: ... minced into 1-2 mm3 pieces and then placed into a digestion solution containing 0.5 mg mL-1 collagenase D (Worthington Biochemical Corporation), 0.01 mg mL-1 DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: Day 25 flushed bovine chimeric embryos were cut into small pieces and treated with 2 mg/ml papain (Worthington Biochemical, LS003119), 1 mg/ml DNase I (Roche ...