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Citations for anatrace :
51 - 93 of 93 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biophysics 2022Quote: ... the membrane fraction was prepared by ultracentrifugation at 100,000 x g for one hour and protein was solubilized by addition of lysis buffer containing 0.5 % LMNG (Anatrace) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... Concentrated PilQ (150 µL of ∼1 mg/mL protein) was exchanged into Amphipol A8-35 (0.585 mg for a 3:1 ratio, Anatrace, A835) and allowed to incubate at 4 C for 1 hr ...
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Protein was extracted via the addition of n-Dodecyl-β-D-maltopyranoside (DDM) with cholesteryl hemisuccinate (CHS) (Anatrace, D310-CH210) to a final concentration of 50 mM DDM / 4 mM CHS and rotated for 1 hour at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
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bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
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bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace, Maumee, OH) and incubating for 1 h at 4° C ...
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bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...
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bioRxiv - Biochemistry 2022Quote: ... protein complexes were overexpressed in BL21(DE3) cells and then extracted from cell membranes using lauryl maltose neopentyl glycol (LMNG; Anatrace). Protein complexes were then affinity-purified using StrepTactin XT resin (IBA ...
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bioRxiv - Biochemistry 2019Quote: Cells were solubilized with dodecyl maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
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bioRxiv - Biophysics 2021Quote: ... coli BL21(DE3) RP cells and purified in DDM (Anatrace) (42) ...
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bioRxiv - Biophysics 2019Quote: ... Cell membranes were solubilized by addition of LMNG or Cymal5-NG (Anatrace) to a final concentration of 0.5 % (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were solubilized by supplementing dodecyl maltoside (DDM) (Anatrace, Sol-grade) to final concentrations of cell at 20 % (w/v ...
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bioRxiv - Biophysics 2021Quote: ... Cell membrane was solubilized in n-dodecyl-b-D-maltoside (DDM, Anatrace) and 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS ...
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bioRxiv - Neuroscience 2019Quote: ... cells were homogenized in the ice cold lysis buffer comprising 1.5% DDM (Anatrace), 10% Glycerol (Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Whole cells were solubilized overnight in 1% Lauryl Maltose Neopentyl Glycol (LMNG Anatrace) and 0.2% Cholesteryl Hemisuccinate (CHS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were solubilized with solubilization buffer containing 1% glyco-diosgenin (GDN) (Anatrace, USA). The wash buffer contained 0.05% [w/v] GDN ...
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bioRxiv - Biochemistry 2023Quote: ... Cell membranes were solubilized with 30 mM n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace). Home-made eGFP nanobody-crosslinked resins (NHS-Activated Sepharose 4 Fast Flow ...
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bioRxiv - Biophysics 2020Quote: ... The cell mixture was extracted with 1% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) for 2 h with stirring at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Lysed cells were solubilized by continuous stirring in 1% L-MNG (Anatrace, Cat. no. NG310) for two hours at 4 °C in the presence of 0.01% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysis was then solubilized in 0.5% (w/v) lauryl maltose neopentylglycol (LMNG, Anatrace), and 0.1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were solubilized in detergent (1 % (w/v) n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace), n-Decyl-β-D-Maltopyranoside (DM ...
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bioRxiv - Biophysics 2020Quote: ... Cell membranes were solubilized in buffer containing 1% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) for 2 h with stirring at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was supplemented with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Cell membranes were solubilized with 1% (w/v) n-dodecyl β-D-maltopyranoside (#D3105GM, DDM, Anatrace) for 1h ...
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bioRxiv - Biophysics 2023Quote: ... The cell mixture was extracted with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, NG310, Anatrace) for 2 hours with stirring at 4℃ and then centrifuged for 1 hour at 30,000g ...
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bioRxiv - Biochemistry 2023Quote: ... Infected Hi5 cells were collected at 72 hr post infection and lysed in 2% CHAPSO (Anatrace) buffer (25 mM Pipes pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed by the addition of 1% v/w n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and by stirring the homogenate for 3 hr at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilization of cell membranes was achieved by incubating with 30 mM n-Dodecyl-β-D-Maltopyranoside (DDM, Anatrace) for 2 h at 4°C under gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... the transfected 293T cells were harvested and lysed in PBS buffer containing 0.3 % n-decyl-β-D-maltopyranoside (DDM, Anatrace). Cell lysates were incubated with Protein A/G PLUS Agarose (Santa Cruz ...
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bioRxiv - Biophysics 2023Quote: ... Cell powder was added at a ratio of 1 g cell powder to 3 ml lysis buffer (200 mM KCl, 21 mM OGNG (octyl glucose neopentyl glycol, Anatrace), 30 mM HTG (n-heptyl-β-D-thioglucopyranoside ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were lysed by cryogenic milling and then solubilized using the detergent n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, solgrade). CST was purified from the clarified lysate using Talon resin (Clontech ...
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bioRxiv - Immunology 2020Quote: ... RBD or S1-binding clones were moved into the ReD cell-display platform (Beasley et al. 2015) and cells were membrane permeabilized using 0.5% n-octyl b-d-thioglucopyranoside (Anatrace, Cat: 0314) and labeled for FACS using either SARS-CoV-2 RBD-Fc (Sino Biological ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells (for SBA clarified lysate preparation) and HEK293T cells (for expression analysis by Western Blot) were solubilized with n-Dodecyl-β-d-maltoside (DM) detergent (Anatrace) to form micelles around membrane proteins and maintain GPCR and RAMP structure and complex formation ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with the Microfluidizer LM20 (Microfluidics) cell disruptor and lysate was incubated with 1% N-Dodecyl-β-D-maltoside (Anatrace) for 1 1hour at 4°C with rocking ...
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bioRxiv - Biophysics 2022Quote: ... Membrane proteins were solubilized from 2.4 L of pelleted HEK293S GnTI-cells expressing wild-type hIP3R3 for 2 hours by rotation in 2% lauryl maltose neopentyl glycol (LMNG; Anatrace Cat# NG310), 150 mM sodium chloride (NaCl) ...