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Citations for anatrace :
1 - 50 of 93 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Biophysics 2021Quote: ... membrane proteins were extracted from the cell with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM Cholesteryl Hemisuccinate (CHS, Anatrace) and 2 μM RTx and incubated at 4° C for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... To reconstitute MBOAT7 proteins in PMAL-C8 (Anatrace), MBOAT7 in detergent was mixed with PMAL-C8 at a 1:3 (w:w ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was extracted by incubating with 1% GDN (Anatrace) on a rotator for 2 hours at 4C ...
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bioRxiv - Biophysics 2023Quote: ... and then solubilized with 4% n-octyl-β-D-glucopyranoside (OG; Anatrace). Solubilized AQP0 was purified using anion-exchange (MonoQ ...
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bioRxiv - Biophysics 2022Quote: SEC-purified protein [in SEC buffer containing 3 mM DM (Anatrace)] was reconstituted into liposomes ...
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bioRxiv - Microbiology 2020Quote: ... Concentrated protein was screened for crystallization with the MCSG Crystallization Suite (Anatrace). Long ...
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bioRxiv - Biophysics 2022Quote: ... Protein was solubilized with the addition of 0.1% (v/v) DDM/CHS (Anatrace) for 2 hours at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to a final concentration of 2% ...
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bioRxiv - Cell Biology 2023Quote: ... Expressed protein was solubilized in 2 % lauryl maltose neopentyl glycol (LMNG) (Anatrace, NG310), 0.2 % Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Neuroscience 2024Quote: ... protein was extracted from the pellet with 0.5% Fos-choline 14 (#F312, Anatrace) at 4°C for 40 minutes and centrifuged at 21 000 g for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was exchanged into 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG, Anatrace) or amphipol A8-35 (Anatrace) ...
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bioRxiv - Biophysics 2020Quote: 3.5 μL of protein-embedded nanodiscs supplemented with 1.5 mM Fos-Choline-8 (Anatrace) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 ...
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bioRxiv - Plant Biology 2021Quote: Thylakoids (100 µg of proteins) were solubilized with 0.75% n-Dodecyl-α-D-maltoside (Anatrace) at RT for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein was extracted by 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Protein extraction was carried out with 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace #D322S25GM) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Microbiology 2019Quote: ... Outer-membrane proteins were solubilized using 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted with a final concentration of 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.2% cholesteryl hemisuccinate (CHS ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... Protein was extracted by addition of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was solubilized by addition of 1% w/v n-decyl-β-D-maltopyranoside (DM) (Anatrace) and 0.1% w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... and membrane proteins were extracted by stirring with 30 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was concentrated to 10 mg/ml using a Proteus X-spinner with 10 kDa filter (Anatrace) and snap frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biochemistry 2019Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 μL of 10% DDM (β-D-dodecyl maltoside, Anatrace) and 13 μL of 27mM mal-PEG (Methoxypolyethylene glycol maleimide ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were extracted by supplementing the lysis buffer with 1% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), and incubated for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010) and 13 µl of freshly dissolved 27 mM 5 kDa mal-PEG ...