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Citations for Qiagen :
101 - 150 of 5872 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... These primers were used to amplify the mRNA from the sample using Quantifast SyBR Green one –step RT-PCR kit (Qiagen, Netherlands) and the reactions were run using ABI 7500 real-time PCR instrument (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Specific pre-mRNAs from total RNA samples were initially amplified by cDNA synthesis (14 cycles) using primers overlapping an intron-exon junction and a OneStep RT-PCR kit (Qiagen, #210212). Each reaction used 250 ng of RNA per reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... while URA3 and ACT1 levels were measured by one-step RT-PCR with specific primers (Supplementary Table S3) following the standard protocol of the RNeasy® Mini kit (Qiagen). All strains were propagated for at least 100 generations before analysis.
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-qPCR were performed using primers previously described by Dias de Melo et al [15] using QuantiNova SYBR® Green RT-PCR Kit (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... RT buffer 5x (4 μL) RT primer mix and Reverse transcriptase (1 μL) (Qiagen, Cat. No. 205311) added in a final volume of 20 μL ...
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bioRxiv - Immunology 2021Quote: ... Relative mRNA expression was evaluated by RT-PCR using the QuantiNova SYBR Green RT-PCR Kit (QIAGEN). Primers of selected cytokines and chemokines used in previous studies (27 ...
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bioRxiv - Microbiology 2021Quote: ... One step RT-PCR was carried out using the QuantiTect probe RT-PCR kit (Qiagen, Valencia, CA) with 500 nM forward and reverse primers and 50 nM labeled probes (DENV2-FAM and ZIKV-FAM and PGK1-VIC TaqMan) ...
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bioRxiv - Pathology 2021Quote: ... Real-time RT-PCR was performed using the QuantiTect Probe RT-PCR Kit (QuantiTect, Qiagen, Venlo, Netherlands) and a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... BAG2 and ATG101 were amplified by RT-PCR from HEK293T cDNA (Qiagen QuantiTect RT-PCR kit, 205311) using primers oNDS227 and oNDS228 for ATG101 ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR for miR quantification was performed with miScript SYBR Green PCR kit (QIagen, 218073). qRT-PCR was run for 40 cycles using the Viia 7 instrument and Viia 7 software was used to extract the raw data (Ct) ...
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bioRxiv - Immunology 2023Quote: ... miScript primers and miScript II RT Kit were purchased from Qiagen. 100 ng total RNA was reverse transcribed according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... with U6 primers included in the miScript II RT kit (QIAGEN) and Gapdh primers provided by Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-PCR was carried out using PyroMark PCR mix (Qiagen) using primers that specifically detect the alternative smaller p53 RNA species ...
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bioRxiv - Neuroscience 2023Quote: Quantitative PCR (qPCR) for miR-519a-3p was performed in triplicate using miRCURY LNA SYBR Green PCR kit (Qiagen) and hsa-miR-519a-3p ...
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bioRxiv - Molecular Biology 2020Quote: ... and miR-324 inhibitors (hsa-miR-324-5p and hsa-miR-324-3p miRCURY LNA miRNA Inhibitor) were obtained from QIAGEN. The sequences of synthetic siRNAs and miRNAs are listed in Supplementary Table S1.
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bioRxiv - Molecular Biology 2023Quote: ... and transfected 24 hours later with 100 nM antisense oligonucleotide targeting miR-194-5p or miR-608 as control (miR-608 is not expressed in Hep G2 cells) (Exiqon, Qiagen) using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: EpiTect Methyl II PCR Primer Assay (Qiagen) was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... hsa-miR-29c-3p and hsa-miR-30b-3p were purchased (Qiagen). Karpas1718 cells (1 million per sample ...
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bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
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bioRxiv - Immunology 2022Quote: ... The AhR mRNA was quantified by one step SYBR Green real-time RT-PCR using the AhR Quantitect primer (Qiagen, Hilden, Germany). The RT-PCR reactions were carried out using the Light Cycler 480 II (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of subgenomic RNA was performed by real-time reverse transcription PCR (RT-PCR) using a QuantiTect Probe RT-PCR Kit (QIAGEN, Hilden, Germany) with primers and probes as previously described [39] ...
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bioRxiv - Cancer Biology 2021Quote: ... or miR-22i (Qiagen) for 48 h to up- or down-regulate intracellular miR-22 ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription and subsequent PCR amplification (RT-PCR1) were performed using the Qiagen OneStep RT PCR mix (Qiagen). The cDNA was selected and unused primers were removed by SPRIselect bead selection (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... Specific primer pairs (miScript Primer Assay or miRCURY LNA miRNA PCR Assay, Qiagen) for miR-142-5p and RNU48 were used ...
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bioRxiv - Molecular Biology 2019Quote: Quantification of miR-9-5p expression in kidney mice samples was performed using the miRCURY LNA RT Kit (Qiagen). Following RT ...
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bioRxiv - Genetics 2019Quote: ... One-Step RT-PCR reagent (Qiagen # 210212) was used to create cDNA and then the cDNA was amplified by PCR ...
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bioRxiv - Microbiology 2022Quote: ... The QuantiNova Probe RT-PCR kit (Qiagen) was used with a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Mx3000/5P thermocylers series (Agilent)-QuantiFast Probe RT-PCR+ROXvial Kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... or QuantiTect Probe RT-PCR Kit (Qiagen) in a 12.5 μl reaction including 2.5 μl extracted DNA or RNA ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR reactions were set up using QuantiTect SYBR Green PCR mix (Qiagen, 204148). Each data point represents three independent biological replicates ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen). Specific primer pairs (miScript Primer Assay or miRCURY LNA miRNA PCR Assay ...
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bioRxiv - Plant Biology 2019Quote: ... RT-PCR products were purified with MinElute PCR Purification Kit (Qiagen). Bulk RT-PCR products were cloned into the barcoded cloning vector and sequenced using the Illumina MiSeq machine ...
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bioRxiv - Molecular Biology 2020Quote: ... miRCURY LNA Power Inhibitor against mouse miR-375 (mmu-miR-375-3p) (Qiagen, Hilden ...
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bioRxiv - Cell Biology 2021Quote: ... 100nM of miR-24-3p inhibitor (Anti-mmu-miR-24-3p, Qiagen, MIN0000219), 100nM of scrambled control (AllStars Negative Control siRNA Print ...
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bioRxiv - Microbiology 2023Quote: ... Primers and probe targeting the AIV matrix gene 36 were used to perform the quantitative RT-PCR (qRT-PCR) reaction by using the One-Step RT-PCR Kit (QIAGEN, Valencia, CA, USA) on a StepOne Real-time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was done with miRCURY LNA RT Kit (Qiagen Cat. No. 339340), adding UniSp6 and cel-miR-39-3p RNA Spike-Ins (Qiagen Cat ...
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bioRxiv - Cell Biology 2021Quote: ... random hexamer primers and reverse transcriptase QuantiTech RT Kit (Qiagen, Germantown, MD). Quantitative PCR was performed using the 7800HT (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... 20 µl RT-PCRs were performed as per the manufacturer’s instructions using the Qiagen One-Step RT-PCR kit (210210, Qiagen) and the following primers ...
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bioRxiv - Cell Biology 2019Quote: ... We then used the purified RNA samples for RT-PCR with the OneStep RT-PCR kit (cat# 210210, Qiagen).
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bioRxiv - Microbiology 2020Quote: ... Expression of the genes of interest was also confirmed using RT-PCR using the OneStep RT-PCR Kit (Qiagen) before the experiment was conducted ...
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bioRxiv - Microbiology 2021Quote: ... and subjected to real-time RT-PCR for viral RNA quantitation [40] using QuantiTect Probe RT-PCR Kit (Qiagen) and QuantStudio 5 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with 200 ng of extracted total RNA using a Qiagen OneStep RT-PCR Kit (Qiagen). The primers used were a forward primer (Hokkaido System Science ...
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bioRxiv - Pathology 2020Quote: ... and the RT product was used for detection of miR-574-5p/3p and control RNA Snord68 using the miScript Primer Assay (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: miScript or miRCURY LNA Primer Assay was used to quantify the expression of miR-146a-5p (MS00003535 or YP00204688; Qiagen) in MIN6 cells or mouse pancreatic islets ...
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bioRxiv - Developmental Biology 2022Quote: ... MiRNA-specific PCR primers were purchased from Qiagen (miR-98-5p ...
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bioRxiv - Biophysics 2019Quote: ... The expression levels of miR-21 were quantified by qRT-PCR using miRCURY SYBR Green PCR Kit (QIAGEN, Venlo, The Netherlands). The results ...
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bioRxiv - Cancer Biology 2023Quote: ... MiRs (61 plus 3 controls) were quantified on a custom PCR plate using the miRCURY LNA miR SYBR Green PCR Handbook (Qiagen, Germany). PCR (40 cycles ...
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bioRxiv - Immunology 2021Quote: ... PRRSV-2 RNA was used to amplify cDNA encoding the full-length of N gene by RT-PCR (One-step RT-PCR, QIAGEN) using specific primers with the forward containing a T7 promoter sequence at the 5’ end ...
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bioRxiv - Microbiology 2021Quote: ... evidence for successful ACE2 transduction was determined by real-time RT-PCR for mCherry mRNA with the OneStep RT-PCR kit (Qiagen). All samples for mCherry analysis were evaluated in one RT-PCR run ...
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bioRxiv - Microbiology 2022Quote: ... primer sets were designed based on the Medaka-polished contigs and used for the RT-PCR amplification of regions of interest using the OneStep RT-PCR kit (Qiagen) according to the manufacturer’s instructions ...