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Citations for Qiagen :
51 - 100 of 5872 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed by QuantiTect SYBR Green RT-PCR kit (Qiagen) using the following primer ...
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bioRxiv - Microbiology 2022Quote: Nested RT-PCRs were performed using the OneStep RT-PCR kit (Qiagen). In each reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... Cel-miR-39 miR mimic (MS00019789, Qiagen) was added to each sample to serve as a spike-in control for monitoring the miR purification and amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... miR-340 or miR-497 (339306, Qiagen) following the manufacturer’s instruction with Lipofectamine RNAiMax (13778075 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: For SYBR Green and Taqman PCR Serial dilutions of known standards were made using synthetic miR-122 (syn has-miR-122-5p, 219600, Qiagen). The dilutions were prepared in triplicate ...
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bioRxiv - Microbiology 2020Quote: ... and RotorGene Q (Qiagen)-OneStep RT-PCR (Qiagen) or QuantiNova Probe RT-PCR kits ...
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bioRxiv - Cancer Biology 2020Quote: ... and miScript Primer Assays (control/normalization:HS-RNU6-2_11, MIR141:HS-miR-141_1, MIR200A:HS-miR-200a_1, MIR200B:HS-miR-200b_3, MIR200C:HSmiR200c_1, Qiagen). Samples ran on a QuantStudio 12K Flex qPCR system (Life Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... Let-7g miR analysis was performed by using Mm_Let-7g_2 miScript Primer Assay (Qiagen, MS00010983) and RNU6-2 (Hs_RNU6-2_11 miScript Primer Assay ...
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bioRxiv - Developmental Biology 2022Quote: ... we treated rat AFSCs at 60% confluence with miR-17-5p and - 20a power inhibitors (25 μM) following the manufacturer’s protocol (Qiagen YI04100215-DDA ...
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bioRxiv - Microbiology 2019Quote: ... The HA and NA influenza segments (complete or fragments) were amplified from cDNA template using subtype-specific set of primers and purified with QIAquick PCR Purification kit (Qiagen). Nucleotide sequences of viral genes were determined using BrilliantDye™v3.1 Terminal Cycle Sequencing Kit (Nimagen) ...
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bioRxiv - Molecular Biology 2020Quote: cDNA synthesis of purified miR-206 was carried out using miRCURY LNA RT Kit (Qiagen) as described ...
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bioRxiv - Genetics 2020Quote: ... reverse primers 852Rb-AGGAAGATAGAGAAAGAGCAACC and 852Rc-AGGAAGATAGAAAAGGAGCAACC using QIAGEN One-Step RT-PCR kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The RT-PCR was performed using Qiagen One-Step RT-PCR kit (Qiagen) master mix ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using a One-Step RT-PCR kit (Qiagen, #210210) using the following primers Forward ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative RT-PCR was performed using Quanti Tect Probe RT-PCR Kit (Qiagen) in a StepOne Plus™ Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and RT-PCR carried out using the One-Step RT-PCR kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed to amplify microRNA 223-3p using the primer hsa-miR-223-3p miRCURY LNA miRNA PCR Assay (Qiagen, #339306, gene Globe ID: YP00205986) and LNA SYBR green PCR kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... miR 155 and miR 224 (Qiagen, HL, Germany) (with sequences as given in supplementary Table S1 ...
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bioRxiv - Microbiology 2019Quote: ... follow manufacturer’s protocol using host and viral primer sets (Table S2) or using QuantiTech Primer Assay (Qiagen) for 18s (Hs-RRN18S_1_SG) ...
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bioRxiv - Molecular Biology 2021Quote: ... miRCURY LNA PCR primers (Qiagen) were used for optimal PCR results ...
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bioRxiv - Genomics 2020Quote: ... End-point RT-PCR was performed using the one-step RT-PCR kit (QIAGEN) following manufacturer’s instructions ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: ... and RT-PCR analysis was performed using a One-Step RT-PCR kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Real-time RT-PCR was performed using a QuantiTec Probe RT-PCR kit (Qiagen) and a LightCycler 480 thermocycler (Roche Diagnostics ...
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Target of Rapamycin Complex 2 modulates development through Hedgehog/Patched signaling in C. elegansbioRxiv - Developmental Biology 2022Quote: ... Quantitative RT-PCR was performed with the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a Bio-Rad CFX96 RT-PCR thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed using the OneStep RT-PCR kit (Qiagen, Cat. No. 210212) with the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed with the Qiagen® OneStep RT-PCR kit (Qiagen, USA). PCR in the second step was performed as described above.
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bioRxiv - Plant Biology 2023Quote: ... for RT-PCR and semiquantitative RT-PCR and an RNeasy Plant Mini Kit (Qiagen) for RT-qPCR RNA gel blots ...
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bioRxiv - Physiology 2020Quote: ... reverse transcription of total RNA containing miRs was performed with miScript II RT kit (Qiagen, UK) and specific primers for miR-26a ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription of total RNA containing miRs was performed with miScript II RT kit (Qiagen, UK). qPCR was performed on a RotorGene™ 6000 (Corbett Research ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen, #210212). The resulting PCR product was sequenced by Sanger sequencing (Genewiz) ...
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bioRxiv - Microbiology 2022Quote: ... The RNA concentration was measured by reverse transcription digital PCR (RT-dPCR) using reported primers and probe 40,41 on QIAcuity dPCR 2-plex platform (QIAGEN) as detailed in the SI.
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bioRxiv - Cell Biology 2019Quote: ... OneStep RT-PCR Kit (Qiagen) was used for reverse transcription of HA fragment with specific primers (Forward ...
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bioRxiv - Microbiology 2020Quote: ... RNA (100 ng) was analyzed by reverse transcription-PCR (RT-PCR) using the OneStep RT-PCR kit (Qiagen). RNA was reverse transcribed and amplified in a total volume of 50 μl containing 2.5 mM MgCl2 ...
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bioRxiv - Immunology 2019Quote: ... The following mouse primer sets were used from Qiagen (Valencia, CA, USA): TBP (PPM03560F ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was run using QuantiFast RT-PCR mix (Qiagen), probes targeting NiVM P gene (5’-ACATACAACTGGACCCARTGGTT-3’ and 5’-CACCCTCTCTCAGGGCTTGA-3’ ...
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bioRxiv - Microbiology 2020Quote: ... Seven overlapping RT-PCR fragments were generated with the One-Step RT-PCR Kit (Qiagen) and purified using the Zymoclean Large Fragment DNA Recovery kit (Zymo Research) ...
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bioRxiv - Genomics 2023Quote: ... Gel-based RT-PCR analyses were performed using the One-Step RT-PCR kit (Qiagen) in a final volume of 10 µL starting from 0.03 µg of DNase- treated (RQ1 Rnase-free Dnase supplemented with Rnasin Ribonuclease Inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... miR-19a-3p and miR-92a-3p LNA probes (Qiagen) were 3’ end labeled with DIG– ddUTP ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription-PCR (RT-PCR) was carried out with total RNA using the one-step RT-PCR kit (QIAGEN) according to the supplier’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: qRT-PCR and WB analyses were performed as described previously (54) using primers described in Supplementary Table S7 and the RT² Profiler PCR Array profiles (Qiagen).
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bioRxiv - Evolutionary Biology 2020Quote: ... we designed strain-specific primers (see Supplementary Table 2) and generated overlapping amplicons using the OneStep RT-PCR Kit (Qiagen) and 5 µL of cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... specific LNA PCR primer assays (Qiagen) for hsa-miR-451a ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using the QuantiFast SYBR Green OneStep RT-PCR kit (Qiagen, Manchester, UK). PCR primers were ordered from Invitrogen Ltd and ThermoFisher-Scientific (London ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then used for RT-PCR using the QIAGEN OneStep RT-PCR kit (Qiagen, Germany) at 50°C for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: RT-PCR was performed on the extracted RNA with One-Step RT-PCR kit (Qiagen, #210212) according to the manufacturer’s instructions and the following conditions ...
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bioRxiv - Cell Biology 2023Quote: Sac2 knockdown was confirmed by quantitative RT-PCR using QuantiTect SYBR Green RT-PCR kit (Qiagen) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... Host gene expression was determined with individual PCR primer sets (gapdh, CAT#QT01658692; tnfa, CAT#QT00104006; gbp2, CAT#QT00106050; cxcl10, CAT#QT00093436; Qiagen USA, Germantown, MD) using Bio-Rad SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and LC480 I (Roche)-QuantiNova Probe RT-PCR Kit (Qiagen); ii ...
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bioRxiv - Immunology 2023Quote: ... Subsequent one-step RT-PCR (Qiagen) was performed using gene-specific primers listed in Supplementary Table S2.
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bioRxiv - Molecular Biology 2019Quote: ... the cDNA template was amplified using microRNA-specific miRCURY LNATM primers for mature miR-9-5p (YP00204513, Qiagen). qRT-PCR was performed as described above ...