Labshake search
Citations for Qiagen :
401 - 450 of 5865 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Each AES was then amplified by PCR on a 3 kb fragment using primer X5 and X6 and purified using QIAquick PCR Purification kit (Qiagen). For gene yrbE4A ...
-
bioRxiv - Microbiology 2020Quote: ... templates containing a T7 phage promoter sequence (5’-TAATACGACTCACTATAGGG-3’) were amplified by PCR using corresponding primer pairs and were purified with a QIAquick PCR purification kit (Qiagen). The primers used are listed in S1 Table ...
-
bioRxiv - Molecular Biology 2022Quote: ... We designed species specific MALAT1 tiling primers for human and green monkey (Table S1) and performed multiplex PCRs following Qiagen Multiplex PCR protocol (Qiagen). cDNA was divided equally between primer sets ...
-
bioRxiv - Cancer Biology 2023Quote: ... Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen, Germany). 2-nd PCR (PCR 2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... MicroRNA qRT-PCR was carried out with custom designed primers to conserved miRNAs (Qiagen) and custom primers from IDT were used to quantify axolotl mRNAs:
-
bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
-
bioRxiv - Microbiology 2020Quote: Bact27F and Bact1492R primers either by directly sequencing agarose-gel purified PCR products (Qiagen), or TA cloning (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Quantitative real time-PCR was performed with specific primers from QIAGEN (Valencia, CA, USA) and detection reagent SYBR Green mix (QIAGEN ...
-
bioRxiv - Cancer Biology 2024Quote: ... Gene-specific primers were used for end-point PCR (HotStarTaq Plus DNA Polymerase; Qiagen), to detect inclusion or exclusion of alternative exons ...
-
bioRxiv - Molecular Biology 2019Quote: ... gambiae miR-276-5p miScript miRNA Mimic (100 ng, Qiagen) at a final concentration of 100 nm were co-transfected into Drosophila S2 cells using FuGENE HD transfection reagent (Promega) ...
-
bioRxiv - Genomics 2021Quote: ... The mmu-miR-122-5p miRCURY LNA probe (YD00615338, QIAGEN) was used to detect the dre-miR-122-5p mature form ...
-
bioRxiv - Physiology 2021Quote: ... Samples were spiked using Cel-miR-39-3p (Qiagen, 219600) and MS2 carrier RNA (Roche ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cells were transfected with miR-22m (Qiagen, Hilden, Germany) or miR-22i (Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... To confirm the expression of miR-130b-p5 (Qiagen, MS00001554) and −3p (Qiagen ...
-
bioRxiv - Neuroscience 2023Quote: ... The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen). Product amplification was performed using the following reaction mixture ...
-
bioRxiv - Microbiology 2020Quote: ... Positive sense vRNA and GAPDH transcripts were measured by qRT-PCR using the QuantiTect SYBR Green RT-PCR kit (Qiagen) on an Applied Biosystems 7300 machine ...
-
bioRxiv - Physiology 2020Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
-
bioRxiv - Immunology 2019Quote: Total RNA samples were diluted to a concentration of 10 ng/μl prior to gene expression studies by qRT-PCR using QuantiFast SYBR green RT-PCR kit (Qiagen) in 7900HT Fast Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Microbiology 2021Quote: ... The remaining 18 μl of RT-PCR DNA was purified by a QIAquick PCR Purification kit (Qiagen, Germantown, MD, USA). Sequences of the purified RT-PCR products were generated using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and viral genome copies quantified by real-time PCR using the OneStep RT-PCR Kit (Qiagen) and the StepOne Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2019Quote: ... Synthetized cDNA was subjected to a PCR array specific for the human antiviral response (RT² Profiler PCR array – PAHS-122Z, SA Biosciences, Qiagen), according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2022Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
-
bioRxiv - Microbiology 2021Quote: ... we used both one-step RT-PCR and two-step (cDNA synthesis followed by separate PCR amplification) reaction designs with commercial One-Step Ahead RT-PCR Kit (Qiagen) or IEH in-house RT ...
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2021Quote: ... The PNPLA2 transcript levels in ARPE19 cells determined by quantitative RT-PCR were normalized using the QuantiTect SYBR Green PCR Kit (Qiagen) in the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed with 100ng of total RNA using the QuantiFast SYBR Green RT-PCR kit (Qiagen, Singapore) detector system (ABI Quant studio 5 ...
-
bioRxiv - Cancer Biology 2020Quote: Quantitative real-time RT-PCR (qRT-PCR) amplification was performed on RNA extracted from cells using RNeasy Mini kit (#74104, Qiagen). RNA (50 ng ...
-
bioRxiv - Neuroscience 2020Quote: ... The quantitative real-time RT-PCR (qPCR) analysis for Bcl2l1 and Bcl2l11 was performed using a SYBR Green PCR Mix (Qiagen) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... Real-time quantitative RT-PCR analysis of the samples was carried out using the mouse antiviral response RT2 Profiler PCR array (Qiagen) on a 7900HT Fast Real-Time PCR system (Thermo Fisher ...
-
bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 75 ng of rRNA was reverse transcribed and amplified with 8 PCR cycles using the OneStep RT-PCR kit (Qiagen) and primers MS2_quant_F and MS2_quant_R (Supplemental Table 5) ...
-
bioRxiv - Cell Biology 2020Quote: ... and run on the RT^2 First Strand Kit (Stem cell PCR array) (Qiagen). The array was run in triplicate (N=3 ...
-
bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Neuroscience 2019Quote: ... All the qPCR experiments were performed using Quantifast SYBR Green RT-PCR Kit (Qiagen) in a LightCycler 480 II System (Roche) ...
-
bioRxiv - Immunology 2019Quote: ... Human RAC2 3’UTR was amplified with One Step Ahead RT-PCR kit (Qiagen) from human mRNA using the following primers RAC2 3’UTR+ ...
-
bioRxiv - Cancer Biology 2021Quote: ... One-step qRT-PCR reactions were performed in triplicate using QuantiTech RT mix (QIAGEN) on the Bio-Rad C1000 Thermocycler and CFX96 system (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed by using QuantiTect SYBR® Green PCR Kit (#204145, QIAGEN) on Agilent MP3005P thermocycler ...
-
bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Microbiology 2019Quote: ... For this purpose we used the QIAGEN OneStep RT-PCR Kit (Qiagen, CA-EUA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Ig Vκ and Vλ from positive wells were amplified by RT-PCR (210210; QIAGEN) and nested PCR (203205 ...
-
bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
-
bioRxiv - Molecular Biology 2021Quote: ... TER1 was amplified from circularized RNA with the OneStep RT-PCR Kit (Qiagen, 210210) (primer sequences in Table S6) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time RT-PCR was performed on a Rotor-Gene Q instrument (Qiagen) using SYBR Premix Ex-Taq II (TakaraBio) ...
-
bioRxiv - Microbiology 2020Quote: ... HIV-1 RNA was analyzed using the QuantiTect SYBR Green RT-PCR kit (Qiagen) in a LightCycler 480 (Roche) ...
-
bioRxiv - Immunology 2021Quote: ... Viral load was quantified by Taq-Man qPCR using OneStep RT-PCR Kit (Qiagen) and AriaMx Real-time PCR Systems (Agilent) ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA (500 ng) was reverse transcribed into cDNA using QuantiTect RT-PCR kit (Qiagen). RT-PCR was performed on a BioRad CFX384 thermocycler using pre-validated Taqman primers (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR mix was prepared using the miRCURY LNA SYBR Green PCR kit (Qiagen). qPCR was performed using the QuantStudio 7 Flex System ...
-
bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in triplicate using the QuantiNova SYBR Green PCR Kit (Qiagen) and the following primer sequences ...