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Citations for Qiagen :
551 - 600 of 5865 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... anti-hsa-miR-181a-5p miScript miRNA Inhibitor (20 nmol, Qiagen, cat# MIN0000256).
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bioRxiv - Cancer Biology 2022Quote: ... 7.5nM of PD-L1-specific miR-455-5p TSB (339194; sequence: GTAGACTATGTGCCTTTGCTCAG; Qiagen) or scramble TSB (339194 ...
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bioRxiv - Genetics 2021Quote: ... Amplification products from semi-quantitative RT-PCR were gel-purified using the QIAquick gel extraction kit (Qiagen) and Sanger sequenced ...
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bioRxiv - Cancer Biology 2019Quote: Real time RT-PCR was performed using total RNA isolated on RNeasy Quick spin columns (QIAGEN, CA). One μg of total RNA was used to perform reverse transcriptase–polymerase chain reaction (RT-PCR ...
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bioRxiv - Genomics 2019Quote: ... Gene expression levels were determined by qPCR using miScript II RT and SYBR Green PCR kits (Qiagen) and results were normalized to the housekeeping gene Hprt1 ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative RT-PCR Array: Total RNA from microfluidic channels was purified using an RNeasy Mini Kit (Qiagen). Next ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA was subsequently transcribed into cDNA using the OneStep RT-PCR Kit from QIAGEN (Catalog # 210212) with the following thermocycler program ...
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bioRxiv - Microbiology 2020Quote: ... A 20 µL reaction mix containing 12.5 µL of 2X Quantitect Probe RT-PCR Master Mix (Qiagen), 0.5 µL of RT mix from the kit ...
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bioRxiv - Microbiology 2020Quote: ... Viral genomic RNA (gRNA) was detected with a one-step real-time RT-PCR assay (Quantifast, Qiagen) using primers and probes generated to target either the SARS-CoV-2 E (28 ...
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bioRxiv - Cell Biology 2021Quote: ... Two micrograms of RNA was used for reverse transcription with Rotor-Gene SYBR Green RT-PCR (QIAGEN) or The SensiMix™ SYBR® No-ROX (BioLine ...
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bioRxiv - Cell Biology 2021Quote: ... Two micrograms of RNA was used for reverse transcription with Rotor-Gene SYBR Green RT-PCR (QIAGEN) or The SensiMix™ SYBR® No-ROX (BioLine ...
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bioRxiv - Immunology 2020Quote: ... Real-time quantitative PCR (RT-qPCR) was performed on a Rotor-Gene Q2plex real-time cycler (Qiagen). For relative gene expressions of IL6 and CXCL8 ...
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bioRxiv - Cell Biology 2021Quote: The RT-qPCR for Miro1 and PCR for mitochondrial DNA was performed on Rotor gene Q (Qiagen). Relative gene expression of Miro1 and mitochondrial DNA copy number were evaluated using their specific forward and reverse primers as indicated in Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... RORC1 and RORC2 gene expression was evaluated by One step SYBR green real-rime RT-PCR (Qiagen) using a Light-Cycler 480 II as follows ...
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bioRxiv - Plant Biology 2023Quote: ... or 500 ng of total RNA for reverse transcription using the One Step RT PCR-Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of RNAs were reverse transcribed and amplified using the OneStep RT-PCR kit (Qiagen Inc.). MTA1 and EF1α (FGRRES_08811 ...
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bioRxiv - Biochemistry 2023Quote: ... The RT-quantitative PCRs were carried out in four biological replicates in a 96-well plate (QIAGEN) using a QIAcuity system (QIAGEN) ...
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bioRxiv - Plant Biology 2020Quote: ... An RNase-Free DNase Set (Qiagen) was used to remove genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Individual set of two siRNAs (Qiagen) against HNF1B or ERG were tested in knockdown efficiency and compared with the siRNA negative-control (Qiagen ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and RNase- Free DNase Set (Qiagen). RNA integrity numbers (RIN ...
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bioRxiv - Immunology 2022Quote: ... and RNase-Free DNase Set (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNase-free DNase Set (Qiagen) was used for on-column DNase digestion ...
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bioRxiv - Genetics 2019Quote: ... and RNase-Free DNase Set (QIAGEN). Adaptors as well as low quality base pairs were trimmed ...
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bioRxiv - Microbiology 2021Quote: ... The RNase-Free DNase Set (Qiagen) treatment was applied to all samples according to the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2023Quote: ... and RNase-Free DNase Set (QIAGEN) according to the product instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and RNase-Free DNAse Set (Qiagen). Total RNA (150 ng ...
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bioRxiv - Immunology 2023Quote: ... with Rnase-Free DNase set (Qiagen). The RNA was eluted with RNAse-free H2O in final volume of 20μl and stored at -80°C.
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bioRxiv - Immunology 2023Quote: ... including RNase-Free DNase Set (Qiagen) treatment ...
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bioRxiv - Immunology 2023Quote: ... and RNase-Free DNase Set (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... including RNase-Free DNase Set (Qiagen) treatment ...
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bioRxiv - Microbiology 2023Quote: ... and RNase-free DNase Set (Qiagen) on-column DNA digestion ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and RNase-Free DNase Set (QIAGEN) according to product instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase-free DNase Set (Qiagen). Infection was confirmed by RT-qPCR using specific primers to detect HDV transcripts.
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Cell Biology 2021Quote: ... Validated qPCR primers and primer assays (Qiagen, see Table 1) were used together with QuantiTect SYBR Green PCR kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.0–3.0 μl (5.0–15 pmol) of primers and 7.5–15 μl of 2x Multiplex PCR Plus Master mix (QIAGEN). The PCR protocol consisted of an initial DNA polymerase (HotStar Taq ...
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bioRxiv - Cancer Biology 2020Quote: ... 1μl of forward/reverse primers (5pmol) and 3μl of nuclease-free water were mixed in a 0.1mL real-time PCR (Qiagen) to conduct PCR in a 10μl of reaction volume ...
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bioRxiv - Genomics 2022Quote: ... and then PCR-amplified with biotinylated primers and pyrosequenced for allele quantification on a Pyromark Q24 system (Qiagen). The same PCR was done on no-reverse transcription control samples to confirm absence of genomic DNA contamination ...
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bioRxiv - Immunology 2020Quote: ... Primers for PCR amplification and sequencing were designed with PyroMark® Assay Design 2.0 software (QIAGEN, Hilden, Germany). PCRs were performed with the IMMOLASE™ DNA polymerase PCR kit (Bioline) ...
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bioRxiv - Cancer Biology 2022Quote: PCR primers for pyrosequencing methylation analysis were designed using the Pyromark Assay Design SW 1.0 software (Qiagen, Germany) (Table 1) ...
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bioRxiv - Microbiology 2022Quote: ... and sgRNA-specific and universal primers (Table 1) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen). sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre) ...
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bioRxiv - Immunology 2023Quote: ... universal primer (Qiagen) and SYBR Green (Roche ...
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bioRxiv - Cancer Biology 2023Quote: CXCL5 primer (Qiagen Mm_Cxcl5_2_SG QuantiTect Primer Assay QT01658146 ...
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bioRxiv - Cancer Biology 2023Quote: ... GAPDH primer (Qiagen Mm_Gapdh_3_SG QuantiTect Primer Assay QT01658692)
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bioRxiv - Molecular Biology 2020Quote: ... FS RNAs and cellular GAPDH pre-mRNAs were done with the QuantiTect SYBR Green RT-PCR Kit (Qiagen), and performed essentially as described previously (56 ...
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bioRxiv - Microbiology 2021Quote: ... Assays were run in a 384-well format using the QuantiFast Pathogen RT-PCR + IC Kit (QIAGEN; 211454). USA/WA-1/2020 SARS-CoV-2 RNA (20,000 genome copies per reaction ...
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bioRxiv - Bioengineering 2020Quote: ... RT-PCR amplifications were carried out using a Power SYBR Green RNA-to-CT 1 step kit (Qiagen) on a ViiATM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of gene expression level was conducted with Rotor-Gene SYBR Green RT-PCR kit (Qiagen, cat# 204174) on the Rotor-Gene-Q PCR cycler (Qiagen) ...