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Citations for Qiagen :
351 - 400 of 717 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and cell pellets were lysed using RLT+ buffer containing 2-Mercaptoethanol using the Qiagen RNeasy+ Micro Kit (Qiagen #74034), following manufacturer’s suggested protocol for RNA isolation ...
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bioRxiv - Microbiology 2020Quote: ... two multiplex PCRs were carried out in a final volume of 12.5 μL containing 6.25 μL of the Qiagen Multiplex PCR master mix (Qiagen, France), 1 μL of DNA template ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2020Quote: ... the tissue samples were homogenized in vials containing 1.4 mm ceramic beads (Fisherbrand) and 400 μl RLT buffer (Qiagen) using a bead mill (Bead Ruptor 24 ...
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bioRxiv - Microbiology 2022Quote: ... One volume of bacterial culture containing approximately 0.2 OD600 of cells was mixed with 2 volumes of RNAprotect (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10,000 TCID50 WT and NS1R38A virus containing inoculum using the QIAamp Viral RNA mini kit (Qiagen), according to the manufactures protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled ORC were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled Cdt1 were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer D with 10 mM imidazole for 1.5 hr with rotation at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of cDNA was then added to a PCR mix containing 12.5 μl 2× Multiplex PCR mix (Qiagen), 9 μl H2O ...
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bioRxiv - Cancer Biology 2020Quote: ... and total RNA was isolated according to the manufacturer’s instructions with following modification: the aqueous phase containing total RNA was purified using RNeasy plus kit (Qiagen). RNA was converted to cDNA with cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Immunology 2022Quote: ... Single cells from three to four mice per each condition were index-sorted directly into 96-well plates containing 5μl of TCL buffer (Qiagen) supplemented with 1%β-mercaptoethanol using a BD FACS Aria II instrument ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was removed and transferred into new sterilized 50ml Falcon tubes containing RNA stabilization buffer (Qiagen, Hilden, Germany). The experiments were performed using three biological replicates ...
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bioRxiv - Plant Biology 2019Quote: ... The multiplex TaqMan qPCR reaction was carried out in 25 μl reaction mixture containing 12.5 μl of Rotor-Gene Multiplex PCR Master Mix (Qiagen), 2 μl of the primer mix (5 µmol l−1 each) ...
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified from plasmid (pSH515) containing the native His4 gene promoter and purified by PCR cleanup kit (Qiagen). DNA was eluted from the spin column with 10 mM Tris-HCl ...
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bioRxiv - Pathology 2020Quote: ... the total RNA (containing small RNAs) was subjected to miRNA reverse transcription using the miScript II RT Kit (Qiagen), following the manual ...
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Cell Biology 2020Quote: For both HSC and MK isolation cells were sorted into wells each containing 2.5ul lysis buffer/well: 950uL RLT Plus buffer (Qiagen) and 50ul 20U/ul SUPERase In RNase inhibitor (Ambion ...
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bioRxiv - Physiology 2020Quote: ... Zero Blunt® Topo or pBluescript SKII (+) vectors containing the ArCCKP cDNA were purified (Qiagen Maxiprep, Qiagen, Manchester, UK) and 5 μg of the vector was linearized using restriction enzymes (NEB ...
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bioRxiv - Physiology 2020Quote: ... Zero Blunt® Topo or pBluescript SKII (+) vectors containing the ArCCKP cDNA were purified (Qiagen Maxiprep, Qiagen, Manchester, UK) and 5 μg of the vector was linearized using restriction enzymes (NEB ...
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bioRxiv - Microbiology 2021Quote: ... while pea-sized tissue samples were placed in 1.5ml screw-top cryotubes containing 500ul of either RNA later or lysis buffer (Qiagen), or without medium ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR amplification was performed in a total volume of 25 µl containing 12.5 µl of Taq PCR Master Mix (Qiagen), 2.5 µl of each primer (10 mM ...
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bioRxiv - Genomics 2019Quote: ... each cDNA amplification reaction was treated with 1µl uracil cleavage mix containing 0.5 µl Uracil DNA Glycosylase (Enzymatics G5010-1140) and 0.5 µl Elution Buffer (Qiagen 19086).
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from peripheral blood and saliva samples containing buccal cells using the Puregene kit (Qiagen, #158389) following the manufacturers’ recommendations.
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bioRxiv - Molecular Biology 2023Quote: ... and sorted into wells of a 96-well plate (185 nuclei/well) containing reverse cross-linking buffer (11µL EB buffer (Qiagen) 0.5µL Proteinase K (Roche) ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 10-day-old whole seedlings grown on 1/2X MS media containing 1% sucrose and 0.8% agar (Plant RNeasy kit (Qiagen)) ...
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bioRxiv - Microbiology 2022Quote: ... and then homogenized tissues in 1 ml PBS containing a penicillin/streptomycin mixture by using a TissueLyser II (Qiagen) at 30-Hz oscillation frequency for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... The filtrated supernatant containing His-thioredoxin-ApoE was applied to a 5 ml Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Pathology 2023Quote: Sixty µl of blood was transferred to a 1.4 mm ceramic beads pre-filled tube containing 600 µL of RLT from the RNeasy Mini Kit (Qiagen), samples were homogenized ...
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bioRxiv - Microbiology 2022Quote: Viral genomic RNA was purified from 280 μL virus-containing media using the QIAamp Viral RNA Mini Kit (Qiagen) as per the manufacturer’s instructions and eluted in 60 μL nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... 14-666-315) containing 1 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Nucleic acids were isolated from excised granulomas by homogenizing in TRIzol (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in tubes containing glass beads (200 mg, 0.4 mm) and were lysed using a TissueLyser LT (Qiagen). To minimize protein degradation ...
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bioRxiv - Microbiology 2023Quote: ... 50000 x g) and the supernatant was filtered and loaded on a column containing Ni2_NTA (nitrilotriacetic) agarose (Qiagen, Netherlands). The column was equilibrated with wash buffer and the bound protein washed ...
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bioRxiv - Neuroscience 2023Quote: ... Individual microglial cells were cut and dropped into an inverted microcentrifuge cap containing 50μl buffer RLT (RNeasy Micro Kit - Qiagen) and 1% β-Mercaptoethanol.
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bioRxiv - Microbiology 2023Quote: ... The RNA-containing phase was then collected and cleaned up using a RNeasy Mini Kit (#74104, Qiagen, Hilden, Germany) with on-column DNase digestion according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... 50 μl of neuronal suspension containing about 7,500 nuclei was used for RNA extraction using RNeasy Mini kit (QIAGEN) and RNase-Free DNase Set (79254 ...
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bioRxiv - Immunology 2022Quote: ... 2 μL of cDNA was added to 19 μL first round PCR reaction mix containing 0.5 U HotStarTaq Polymerase (Qiagen), 0.18 μM of each 3’ reverse primer ...
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bioRxiv - Developmental Biology 2023Quote: Blastocysts were washed with DPBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted with TRIzol and the upper phase containing RNA was loaded into RNeasy columns (Qiagen, #74004) after the addition of isopropanol ...
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bioRxiv - Bioengineering 2023Quote: ... Culture broths were taken and transferred into each tube containing two volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany). Samples were pelleted as manufacturer’s instructions and stored at −80°C freezer until extraction ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrated supernatant containing His-tagged NanoLuc was applied to a 5-mL Ni-NTA Superflow Cartridge (Qiagen, Germany) pre-equilibrated with TBS buffer A ...
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bioRxiv - Microbiology 2024Quote: ... The amplification was performed in a 25 μl reaction volume containing 12.5 μl of Rotor-Gene Multiplex PCR Master Mix (Qiagen), 2 μl of the primer mix (5 µM each primer concentration ...
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bioRxiv - Microbiology 2024Quote: ... The combined tissues were submerged in 600μl of lysis buffer containing 30 μl of foaming reagent (Reagent DX, Qiagen) and 60μl of β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... the mouse anti-penta-His antibody (Qiagen; Catalog #34660) was used at a 1:2,000 dilution in 3% BSA in PBS with sodium azide ...