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Citations for Qiagen :
551 - 600 of 717 citations for Tripartite Motif Containing 22 TRIM22 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 HEK-293T cells were transfected with 2 μg of plasmid DNA containing each shRNA along with packaging plasmids pMD2.g and psPax2 using Effectene® Transfection Reagent (QIAGEN) to yield 5 mL of supernatant containing lentivirus ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with the plasmid containing the Cdt1 gRNA/Cas9 and the Cdt1 repair template at equimolar ratio using Effectene (Qiagen, #301425) transfection agent ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen, cat# 76506) and centrifuged for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... The solubilized membrane fraction was loaded on a column containing 0.5 mL Strep-tactin Superflow plus resin (50% suspension; Qiagen, Hilden, Germany) equilibrated with 4 mL of the Wash buffer ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 2-times and 1 μl was taken for each PCR reaction (20 ul) containing: 0.02 U HotStarTaq® DNA polymerase (Qiagen, Germany), 1 x PCR buffer ...
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bioRxiv - Immunology 2022Quote: ... The top 4uL of lysis supernatant containing the RNA fraction was directly placed into 350uL of RLT buffer from an RNEasy micro kit (Qiagen 74004), and RNA was purified the following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and the pellet containing virions was resuspended in 200 μl UltraPure water and added to the QIAGEN DNeasy PowerSoil Kit bead tubes (QIAGEN, Germany) for DNA extraction according to the manufacturer’s instructions with one exception ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed for all primers in 10μl reaction containing 4μl Qiagen multiplex PCR buffer mix (QIAGEN Inc., Hilden, Germany), 0.3 μM primer mix for each set ...
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bioRxiv - Genomics 2022Quote: The second lobe of lung or trachea was immersed in 1 mL PBS in a 2 mL Micro Centrifuge Tube (Fisherbrand, 14-666-315) containing one stainless steel bead (5 mm, QIAGEN, 1026563) immediately after dissecting the SARS-CoV-2 infected mouse or hamster ...
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bioRxiv - Genomics 2022Quote: ... Trachea was dissected and immersed in 1 mL PBS in a 2 mL microcentrifuge tube (Fisherbrand, 14-666-315) containing one stainless steel bead (QIAGEN, 69989). After the homogenization ...
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bioRxiv - Genetics 2019Quote: ... The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s ...
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bioRxiv - Neuroscience 2019Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80°C until clean up using a kit (Qiagen 154025593). cDNA was generated using 11 rounds of amplification with 10 ng RNA input.
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bioRxiv - Immunology 2020Quote: ... cells were lysed in RLT lysis buffer containing β-mercaptoethanol and total RNA extracted using the RNeasy micro kit (Qiagen, USA). Total RNA was assessed for quality and quantified using a total RNA 6000 Nano LabChip on a 2100 Bioanalyzer (Agilent Inc. ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were generated by transfecting the DNA constructs containing the gene of interest with Effectene transfection reagent (Qiagen, Hilden, Germany), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Cells within plugs were lysed in 2ml cell lysis buffer (Bionano Genomics, San Diego, CA) containing 167μl proteinase K (Qiagen, Germantown, MD) for 16 hours at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Physiology 2020Quote: ... A cross-sectional piece of kidney containing cortex and medulla was homogenized in Buffer RLT with 0.01% β-ME and further homogenized with Qiashredder columns (Qiagen 79654). mRNA concentration was measured by Nanodrop (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80 °C until clean up using a kit (Qiagen 74004). cDNA was generated using 11 rounds of amplification with 10 ng RNA input ...
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bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen, 1018244) and bound proteins were eluted in lysis buffer lacking EDTA and DTT but containing 10 mM maltose ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD14- CD16- CD20+CD38+ BG505 SOSIP+YU2-gp140+ B cells were sorted into individual wells of a 96-well plates containing 5 μl of a lysis buffer (Qiagen, 1031576) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were transferred to a new 0.5ml tube and incubated in 100 μL ChIP elution buffer (10mM Tris-HCl pH8.0, 5mM EDTA, 300mM NaCl, 0.5% SDS) containing 5 µL proteinase K (Qiagen, 20mg/ml stock) at 55°C for 2 h and then at 65°C for 4 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue lysis was performed by re-suspending ∼30mg of sliced frozen tissue in a solution containing 1% β-mercaptoethanol in RLT buffer (supplemented with antifoam agent; ID 19088, Qiagen). Next ...
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Microbiology 2022Quote: ... containing RNA was transferred to 1.5 mL tubes containing 500 µL of ice-cold ethanol and shaken vigorously before loading into the RNeasy Mini spin columns (Qiagen, Germany). Subsequent RNA extraction steps were performed according to the manufacturer’s protocol of the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... ∼50mg of each thawed sample was transferred to a microcentrifuge tube containing a 5mm stainless steel bead (Qiagen, Part No. 69989) and 1200 µL Buffer RLT Plus ...
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Immunology 2022Quote: ... Tissues were thawed and tissue pieces were transferred to clean 2mL tubes containing 600uL RLT Buffer from the RNeasy RNA Mini kit (Qiagen # 74136) and 2mm beads ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were rapidly placed inside of a RNAse-free Eppendorf tube (Dominique Dutscher, reference #033872) containing 200μL of buffer RLT Plus (Qiagen, reference #1053393), and immediately frozen on dry ice ...
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bioRxiv - Immunology 2022Quote: ... following the final boost (Week 18 or 23) were sorted into individual wells of a 96-well plate containing 5 μL of lysis buffer (TCL buffer (Qiagen, 1031576) with 1% of 2-β-mercaptoethanol) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clarified supernatant containing mRFP1 was then purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The elution from the gravity column was concentrated and buffer exchanged (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... and the RNA-containing aqueous phase was subjected to a column purification kit using RNeasy Mini RNA extraction kit (Qiagen, USA). RNA was DNase treated ...
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bioRxiv - Biochemistry 2023Quote: ... after 1 in 4 dilution to each sample prior the extraction and then homogenised samples in 200 μL ice-cold 60% methanol (MeOH) containing 0.01% (w/v) butylated hydroxytoluene (BHT) by using TissueLyserII (Qiagen, Hilden, Germany) at 25 Hertz for one minute and incubating for 20 minutes in an ultrasonic bath (Soniclean Pty Ltd. ...
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bioRxiv - Microbiology 2023Quote: ... 20-40 mg of tissue was placed in a tube containing 600 μl of OptiMEM and a 5 mm stainless steel bead (Qiagen; #69989) and homogenized in the Qiagen TissueLyser II by two dissociation cycles (1,800 oscillations/minute for 2 minutes ...
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bioRxiv - Biophysics 2023Quote: ... beads were discarded then and the supernatant containing reconstituted into nanodiscs A2AAR was incubated with 250 μL of Ni-NTA resin (Qiagen, Germany) (2 h with constant rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells containing the desired plasmids were amplified by broth culture and the plasmids were prepared by maxiprep kit (Qiagen, 12663). The plasmids were stored at −20°C until use.
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bioRxiv - Evolutionary Biology 2023Quote: ... The complete set of 15 loci were amplified in four separate 15 μL multiplex-PCR reactions containing 7.5 μL of 2X Master Mix (Type-It Microsatellite PCR kit, Qiagen, Venlo, Netherlands), 0.015-0.15 μL of unlabeled forward primer with tail (10 μM) ...
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bioRxiv - Neuroscience 2023Quote: ... we transferred the microdissected tissue to RNase-free microcentrifuge tubes containing ice-cold RNAprotect reagent (catalog # 76104; Qiagen; Venlo, The Netherlands). We stored the tissue samples in RNAprotect at 4□ overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Beads/cells were placed on a magnetic stand and the supernatant containing DNA fragments was transferred into a new tube for DNA purification (MinElute Qiagen, 28004). The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek ...
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bioRxiv - Bioengineering 2024Quote: ... posterior eye cups (PECs) were separated and collected in 2 mL reinforced tubes (SPEX Sample Prep) containing 5 mM stainless steel beads (Qiagen, LLC).
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bioRxiv - Bioengineering 2019Quote: ... The bacterial pellet was processed to purify all containing DNA by alkaline lysis and column purification (Qiagen miniprep spin kit, Qiagen, Inc., Germany).
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bioRxiv - Microbiology 2019Quote: ... The resulting 11.4kb HIV vector-containing fragments free of plasmid backbone were purified from agarose using QIAquick Gel Extraction Kit (Cat No./ID: 28706 Qiagen, Germantown, MD). A 304 bp zip code-containing insert fragment pool was generated by PCR using NL4-3 GPP or GPV- as template ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting supernatant containing soluble LCC was subjected to purification by immobilized metal ion chromatography (IMAC) using Ni-NTA Superflow (Qiagen, Hilden, Germany). Protein elution using 250 mM imidazole was then dialyzed against 100 mM potassium phosphate buffer (pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: The genomic DNA was extracted from the blood and the microfilaria-containing tissues using the QIAGEN DNA tissues extraction kit (QIAGEN, Hilden, Germany), following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: ... the pCMV-Neo-Bam p53 R248W vector containing the ampr gene was purified from the transfected SKOV-3 cells using the Qiaprep Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... existing media was removed and the resulting myotubes received either 750 µl of fresh DM alone or 750 µl DM containing 9 µl HiPerFectTransfection™ (Qiagen, UK) and 20 nmol Flexitube GeneSolutions™ (siUBR5 ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged proteins were recovered from the clarified lysate using a drip column containing a 5.0 ml bed volume of Ni-NTA resin (Qiagen, Mississauga, ON, Canada). After extensive washing with buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1,000 T-cells/CAR T-cells were FACS-sorted in single PCR tubes containing 4 ul of QIAGEN lysis buffer from QIAseq FX Single Cell RNA Library Kit (QIAGEN, Hilden, Germany) and stored at −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and areas containing >30% tumor cells as determined by an expert pathologist were macrodissected and deparaffinated using Deparaffinization Solution (Qiagen, Hilden, Germany). DNA was extracted and purified with GeneRead DNA FFPE Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...