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Citations for Qiagen :
51 - 100 of 1216 citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... HIS-tag (Qiagen 34610 1:100) and HER3 (R&D Systems AF4518 1:400) ...
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bioRxiv - Biophysics 2023Quote: ... anti-Strep-tag antibody (Qiagen, 34850), or anti-HA-tag antibody (Cell Signaling ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was extracted from 10 ul of the purified virus using the MinElute Virus Spin Kit (Qiagen Cat#57704), and the viral genome titer was determined by qPCR using an AAV2 rep gene specific primer probe set (repF ...
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bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... For next generation sequencing RNA from virus-containing samples were extracted using the QIAsymphony DSP Virus/Pathogen mini kit (Qiagen). RNA was DNase-treated using the TURBO-free Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Whole genome sequencing of the isolated virus was done isolating viral RNA using the QIAamp MinElute Virus Spine kit (Qiagen) and performing Illumina sequencing ...
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bioRxiv - Genetics 2022Quote: ... The QIAamp MinElute Virus Spin Kit (QIAGEN, Germany) was used for viral nucleic acid extraction ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-His tag (1:2000; QIAGEN) and rabbit anti-GST (1:2000 ...
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bioRxiv - Genetics 2020Quote: ... or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... QIAamp MinElute virus spin kit (Qiagen Sciences, Maryland, MD) was used to extract total nucleic acids from each wastewater sample as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and a dedicated kit (QIAsymphony DSP Virus/Pathogen, Qiagen). All the extracts were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... Equine arteritis virus (EAV) in AVL lysis buffer (Qiagen) was spiked into the reagent as internal control for extracellular RNA samples ...
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bioRxiv - Microbiology 2021Quote: ... Virus RNA was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... using the “QIAamp Min ELUTE Virus Spin” Kit (QIAGEN), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using the QIAamp 96 Virus kit QIAcube HT (Qiagen). To detect SARS-CoV-2 RNA ...
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bioRxiv - Microbiology 2021Quote: ... whole ticks were bisected using a sterile 16-gauge needle and incubated in a lysis buffer (i.e., 180 μl Qiagen Buffer ATL and 20 μl proteinase K) at 56°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: Lineage tracing plasmids and envelope-expressing plasmids were prepared using a plasmid maxi kit (QIAGEN cat. 12162). To produce lentivirus ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was extracted from the challenge virus inoculum and virus-positive clinical samples using the QIAamp viral RNA mini kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... we also probed for the Strep-tag on γTuNA (1:1000 dilution of Strep-tag mouse monoclonal antibody, cat. # 34850, Qiagen). Band intensities were measured in ImageJ and normalized in Prism 7 by the wildtype γTuNA band (positive control) ...
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bioRxiv - Microbiology 2020Quote: ... a monoclonal mouse Strep-tag antibodies (#34850, Qiagen), a monoclonal anti-actin antibody (A5441 ...
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bioRxiv - Microbiology 2020Quote: ... resulting in an N-terminal 6xHis-tag (Qiagen) for each construct and were then cloned into E ...
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bioRxiv - Bioengineering 2023Quote: ... before his-tag purification with Ni-NTA (QIAGEN) and wash and elution buffers of the same composition as the solubilization buffer ...
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bioRxiv - Microbiology 2019Quote: ... using the EZ1 Virus Mini Kit v2.0 (Qiagen Hilden, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... and QiaAmp 96 Virus QiaCube HT kit (Qiagen, Cat. 57731). RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated using EZ1 Virus Mini Kit v2.0 (QIAGEN). Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche ...
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bioRxiv - Immunology 2020Quote: ... from mouse plasma using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted using the QIAamp MinElute Virus Kit (Qiagen, #57704). Purified DNA was eluted in 50μl of Ultra-Pure Water (UltraPure™ DNase/RNase-Free Distilled Water ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using QIASymphony DSP Virus Pathogen Midi kit (Qiagen) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Cancer Biology 2021Quote: 293FT cells were transfected with pMSCV-dnCRTC or pMSCV-GFP constructs together with packaging plasmid pMD.MLV and pseudotyped envelope plasmid pMD2-VSV-G using Effectene transfection reagent (Qiagen #301425) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... microdissected human brain tissue was flash frozen and lysed to extract proteins using a TissueLyser II system (QIAGEN) and total protein concentration determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific 23225) ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
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bioRxiv - Zoology 2021Quote: Nucleic acids were extracted using a QIAamp MinElute Virus Spin Kit (QIAGEN) and used to construct the sequencing libraries ...
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bioRxiv - Plant Biology 2021Quote: ... Virion DNA was isolated using QIAamp MinElute Virus Spin Kit (Qiagen, Maryland).
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bioRxiv - Immunology 2020Quote: Plasma RNA was extracted using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011) or ddPCR using One-Step RT ddPCR Adv kit (Bio-Rad 1864022) ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011) or ddPCR using One-Step RT ddPCR Advanced Kit for Probes (Bio-Rad 1864022) ...
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bioRxiv - Microbiology 2023Quote: ... and recLI9-NS1-GFP11 reporter virus was purified using RNeasy kit (Qiagen). cDNA synthesis was performed as above using random hexamer primers (25°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen). Then ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Strep-tag II epitope mouse monoclonal antibody # 34850 (QIAGEN), anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: anti-STrEP-Tag (#34850, Qiagen), monoclonal anti-β-actin antibody (A5441 ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Molecular Biology 2019Quote: ... and virus RNA was purified by using the QIAamp viral RNA kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was extracted from the virus preparation using the RNeasy kit (Qiagen). The first cDNA strand corresponding to the entire 10 kb-long genomic RNA was produced using Superscript III Reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from virus stock using QIAamp Viral RNA Mini Kit (Qiagen) and treated with TURBO DNase (Ambion) ...
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bioRxiv - Immunology 2019Quote: Viral RNA was isolated from virus particles with RNeasy-Kit (Qiagen, Valencia, CA). Access RT-PCR kit (Promega ...
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bioRxiv - Neuroscience 2020Quote: SIV RNA was measured by qRT-PCR using the QuantiTech Virus Kid (Qiagen) and the following primers and probes in the SIV gag region (Fwd ...