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Citations for Qiagen :
1 - 50 of 1216 citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Immunology 2022Quote: ... To sequence the envelope from serum virus we isolated viral RNA (Qiagen), synthesized cDNA ...
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bioRxiv - Epidemiology 2019Quote: ... and then individual ticks were homogenized with TissueLyser LT (Qiagen, Germany). Thereafter ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the tick using DNeasy Blood & Tissue kit (Qiagen) and was submitted to Yale Center for Genome Analysis for Illumina (NovaSeq ...
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bioRxiv - Molecular Biology 2019Quote: ... Total DNA was isolated from individual ticks using QIAamp DNA Mini Kit (Qiagen, Germany), following its protocol ...
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bioRxiv - Microbiology 2019Quote: ... ticks were washed in ethanol and homogenised in lysis buffer using a TissueRuptor (Qiagen) and RNA was extracted using the RNeasy plus mini kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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bioRxiv - Microbiology 2022Quote: ... 6XHis-tag fusion proteins were purified using Ni-NTA affinity chromatography (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... His-tag proteins were purified using a Ni-NTA purification system (Qiagen) by following the specification of the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Tick DNA was extracted either using the BioSprint All Wet Vet Kit (Qiagen, Hilden, Germany) or a Maxwell® 16 LED DNA kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Virus RNA virus was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from each tick pool with the RNeasy Mini Kit (QIAGEN, Hiden, Germany). According to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA extraction from tick cell lines was performed using TRIzol LS and RNeasy-spin columns (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and total RNA was extracted from tick pools with an RNeasy Mini Kit (QIAGEN, Hiden, Germany), followed by cDNA synthesis using M-MLV reverse transcriptase (TaKaRa Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each tick using the DNeasy Blood and Tissue kit (#69506, Qiagen, Germantown, MD). Samples were submitted for 16S rRNA sequencing of the hypervariable region v6 on the Illumina MiSeq platform to assess the baseline species composition of field-collected blacklegged ticks.
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bioRxiv - Microbiology 2020Quote: ... Total RNA from 16 tick pools was then extracted using the RNeasy® Plus Universal kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from these whole ticks using a 96-well DNeasy Tissue Kit (Qiagen, Valencia, CA, USA) modified for use with the QIAvac vacuum filtration system ...
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bioRxiv - Biochemistry 2020Quote: ... The His-tag protein was purified by using a column of Ni-Sepharose 4B (Qiagen), followed by gel filtration using a Superdex 75 column (G.E ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from the tick homogenates using the Qiagen DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies against His-tag and S-tag were purchased from Qiagen and GenScript respectively ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA and/or enriched small RNA fractions were isolated from whole-tick samples using the miScript microRNA isolation kit according to the manufacturer’s instructions (QIAGEN, China). The quantity and integrity of the total RNA was assessed using an Agilent 2100 Bioanalyzer system (Agilent Technologies ...
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bioRxiv - Zoology 2020Quote: ... Genomic DNA was extracted from each individual tick homogenate using a DNeasy blood and tissue kit (Qiagen, Valencia, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: His-tag purified recombinant proteins were expressed in E.coli and purified by Ni+-NTA-agarose column (Qiagen). Then ...
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a TEV cleavable N-terminal His-tag were purified using Ni-NTA agarose (Qiagen) resin and were treated to gel filtration using a Superose 6 Increase 16/600 column or Superdex 200 Hiload 16/600 column (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... hexa-histidine tag (Qiagen), GFP (Santa Cruz Biotechnology ...
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bioRxiv - Evolutionary Biology 2019Quote: ... QIAsymphony DSP virus/pathogen Kit (Qiagen) for blood samples ...
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bioRxiv - Microbiology 2023Quote: ... with EZ1 DSP Virus kit (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... using Virus Extraction Mini Kit (Qiagen). The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific ...
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bioRxiv - Epidemiology 2020Quote: ... then ticks were washed by gentle shaking over 2-3 min at 7 Hz/s in a Tissue Lyzer (Qiagen, Germany). After discarding the supernatant ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from 50 individual ticks using the QIAamp DNA Mini Kit according to the manufacturer’s protocols (QIAGEN, Shanghai, China). The extracted DNA was stored at -20°C until use.
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Zoology 2021Quote: ... unfed and partially engorged tick tissues (salivary gland, midgut, ovary) from PA only with the DNeasy Blood and Tissue kit catalog # 69506 (Qiagen, Valencia, CA) using th standard protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... anti-Strep-tag (34850, Qiagen), anti-HSP90 (4874 ...
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bioRxiv - Biochemistry 2019Quote: ... anti-Strep-tag (34850; Qiagen), anti-His-tag (11922416001 ...
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bioRxiv - Immunology 2024Quote: ... anti-Strep Tag (Qiagen 34850), anti-MLKL (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... anti-strep tag (Qiagen, 34850), anti-6*his tag (Thermo Fisher Scientific,11533923) ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from virus supernatants using QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). qPCR was then performed using AgPath RT-PCR (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted from SIV and SIVΔNef virus stocks with the QIAamp UltraSens Virus kit (Qiagen, Germantown, MD), reverse transcribed with the Superscript VILO kit (Thermofisher ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose, Qiagen) following the purification hybrid method from the ProBond purification system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... with the Virus Mini Kit v2.0 (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... ZIKA Viral RNA was isolated from Zika virus stock using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) to create a standard curve using serial 10-fold dilutions of ZIKV RNA ...
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bioRxiv - Microbiology 2022Quote: ... copies were extracted from plasma virus using the Qiagen BioRobot EZ1 Workstation with EZ1 Virus Mini Kit v2.0 (Qiagen). Eluted vRNA was subsequently used as a template for cDNA synthesis and reverse-transcribed using the reverse primer SHIV.Env.R1 (5’-TACCCCTACCAAGTCATCA-3’ ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) as fusion proteins containing an N-terminal His6- tag and purified by immobilized metal-affinity chromatography (Ni-NTA, Qiagen). Full-length DosR was also expressed with N-terminal GST-tag ...
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bioRxiv - Immunology 2019Quote: ... StrEP-Tag (mouse monoclonal, Qiagen, #34850). Peroxidase-conjugated secondary antibodies against rabbit IgG (#7074 ...