Labshake search
Citations for Qiagen :
301 - 350 of 351 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Cancer Biology 2019Quote: ... Germany) using a metal ball for 2×2 min at 25 s−1 in 600 μl of RLT buffer (Qiagen, Germany) with 1% β-mercaptoethanol ...
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bioRxiv - Genetics 2020Quote: ... we split the 50 μl reaction volume into two and we added 25 μl of guanidine hydrochloride (Buffer PB, Qiagen 28606) to each as a chaotropic agent to stop the reaction and dissociate the proteins and transposase from the DNA ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted with 1 volume of 25:24:1 phenol-chloroform-isoamyl alcohol and purified with QIAquick PCR Purification Kit (QIAGEN 28104). Libraries were prepared with the Ovation Ultralow V2 DNA-Seq Library Preparation Kit (NuGEN (Redwood City ...
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bioRxiv - Microbiology 2020Quote: ... or remaining mosquito bodies were collected separately in a 2ml Eppendorf Safe Lock tube with 250μl DMEM (2% FBS, 1% Sodium Pyruvate) with 25 mg/ml Amphotericin B and a stainless steel bead (Qiagen, Hilden, Germany). Samples were stored at −80°C.
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bioRxiv - Immunology 2021Quote: ... Freshly harvested or in -80°C stored organ in 1 ml complete cell culture medium was homogenized using a metal bead at 25 Hz for 1 - 1.5 min by TissueLyser (Qiagen, Hilden, Germany). After centrifugation at 3500g for 5 min ...
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bioRxiv - Genomics 2022Quote: ... The sample was incubated at room temperature for 5 min and 3.75μL (25 fmol final concentration) of the synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples ...
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bioRxiv - Neuroscience 2022Quote: Dissected cortex and striatum were homogenized 2 x 1min at 25 Hz in 750μL of QIAzol Lysis Reagent (Qiagen, Valencia, CA) with TissueLyser (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... polymerase chain reactions (PCRs) were performed in a final volume of 25 µL using the Taq DNA Polymerase from Qiagen (www.qiagen.com) with a modified mix proportions as followed ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 x 2 minute intervals at 25 Hz with the addition of one 7 mm stainless steel bead (Qiagen #69990). 200 uL of tissue lysate was used for KingFisher Flex (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... a small amount of tissue (>25 mg) was added to 1 mL of TRIzol with a single 5mm stainless steel bead (Qiagen, Germany) and homogenised using a TissueLyser II (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... UK) were prepared using genomic DNA extracted from approximately 25 male beetles using the Qiagen MagAttract HMW DNA Kit (Qiagen, Germany) and run on MinION flow cells at the Millard and Muriel Jacobs Genetics and Genomics Laboratory ...
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bioRxiv - Biophysics 2023Quote: Genomic DNA was isolated from 25 mg of frozen septum samples by using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... was added to each sample tube and homogenization was conducted with two shaking steps at 25 Hz for 2 min by TissueLyser (Qiagen, USA). RNA extraction was performed with the Direct-zol RNA MicroPrep Plus kit (Zymo Research ...
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bioRxiv - Developmental Biology 2022Quote: We collected 25 mg of tissue and we purified DNA using the QIAamp Fast DNA Tissue Kit from QIAGEN (Cat. No. 51404). We verified the integrity of the DNA using 1% agarose gels ...
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bioRxiv - Bioengineering 2019Quote: ... Up to ∼2 million cells were resuspended in 100 μl High-TE buffer (10 mM Tris-Cl, pH 8, 10 mM EDTA, 25-100 μg/ml RNase A (QIAGEN, Cat. # 19101)) and lysed by adding 2.5 μl 20% SDS ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from a quarter of blood spot (approx. 25 μL) using the QIAamp DNA blood Mini Kit (QIAGEN, Hilden, Germany) with a modified procedure.37 The kelch13 gene propeller domain sequence was determined by capillary sequencing of PCR products ...
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bioRxiv - Microbiology 2020Quote: ... Plasmodium falciparum DNA was extracted from a quarter of a blood spot (25 µL) using the QIAamp DNA blood Mini Kit (QIAGEN, Hilden, Germany).
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bioRxiv - Neuroscience 2021Quote: ... The DNA was extracted from a small portion of the sample (∼25 mg) using the Qiagen DNeasy kit (Qiagen, German-town, MD). The mitochondrial COXI genes were amplified using Folmer’s universal COXI primers LCO1490 (5’-GGT CAA CAA ATC ATA AAG ATA TTG G ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was further treated with 25 µL of proteinase K (20 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19133), gently mixed by flicking of tube and incubated at 56 °C for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Real Time PCR reaction was carried out in a total of 25 µl reaction using Rotor-Gene Multiplex PCR kit (Qiagen, Hilden, Germany) and a final concentration of primer and probe at 0.5 µm and 0.2 µm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted from up to 25 mg tissue with an extraction robot and the DNAeasy Blood & Tissue kit (Qiagen, Hilden, Germany) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2 μL of fecal DNA from each mouse was used as template in 25 μL total reactions of SYBR Green Real Time PCR Master Mix as instructed by the manufacturer (Qiagen; Hilden, Germany). Reactions were carried using CFX Connect Real Time PCR Detection System (BioRad ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Each primer (3.5 μl) was combined with reverse-transcriptase reagent (1 μl) and SYBR® Green master mix (25 μl) (Qiagen, Valencia, CA). The thermal profile was as follows ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR reactions were performed in triplicates in a total volume of 25 µL with 1X of PCR buffer (including 3mM MgCl2 and 400 μM each dNTP, QIAGEN® Multiplex PCR kit, Qiagen, Germany), 0.5 µM of each primer and 10 ng of template DNA for bulk DNA or 5 µL of template DNA for etDNA ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 25 mg of homogenized tissue from each sample using the DNeasy Blood & Tissue Kit (Qiagen, catalog number 69506) and eluted in 30 μl of Buffer AE ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.1 g of tissue per 1 mL of PBS was homogenized with stainless steel beads of 5 mm (25–30 Hz for 5 min) by a Tissuelyser (Qiagen, Venlo, Netherlands). The homogenates were centrifuged at 10,000 x g for 10 min and the supernatant was collected and stored at −80 °C until CD quantification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bisulfite converted DNA (∼20 ng) was amplified by PCR in a 25 μl reaction volume using HotStartTaq plus DNA polymerase (Qiagen; Germantown, MD) with 1.5mM MgCl2 and 0.12 μM each of the forward and reverse primer (Supplemental Table 2) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA extracts with visible impurities (green or brown colour; ~25% of samples) were subsequently purified using the DNeasy PowerClean Pro Cleanup Kit (Qiagen, Hilden, Germany). Genomic DNA was stored in the DNA bank of Naturalis Biodiversity Center ...
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bioRxiv - Microbiology 2023Quote: Each PCR amplification method was performed in a 25 µl reaction volume using HotStar Taq Plus Master Mix kit (Qiagen, Hilden, Germany). Details of primers used in this study are given in the Table 1.
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bioRxiv - Genetics 2023Quote: ... amplifications were performed in reaction volumes of 25 μL using a reaction mix containing 1x Type-it Multiplex PCR Master Mix (Qiagen, Gmbh, Hilden), 0.2 μM of each forward and reverse primer pair ...
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bioRxiv - Microbiology 2023Quote: ... in-house mock community (25 diverse Gram-positive and Gram-negative bacteria and fungi) and a negative reagent control (nuclease-free water, Qiagen, Hilden, Germany) were included ...
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bioRxiv - Systems Biology 2023Quote: ... a total of 600 µL of cold 2:1 methanol:chloroform was added and homogenized in a bead-based tissue homogenizer at 25 Hz for 4 minutes (TissuLyser II, Qiagen, Hilden, Germany). 200 µL each of water and chloroform were then added ...
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bioRxiv - Cancer Biology 2023Quote: ... we adapted the same conditions used in Dip-C40 and lysed each nucleus in 150 nL of lysis buffer containing 20 mM Tris pH8/20 mM NaCl/25 mM DTT/0.15% Triton X-100/1 mM EDTA/5 µg/mL Qiagen Protease (Qiagen, cat. no. 19157). After dispensing ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of the template was used in a 25 μL reaction using the QuantiFast Probe RT-PCR Kit (Qiagen, Inc., Valencia, CA) with 0.4 μM of each primer and 0.2 μM probe ...
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bioRxiv - Microbiology 2019Quote: Total DNA was extracted from the same 25 isolates showing similar AMR phenotypic profiles using the DNeasy® Blood & Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer's recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... 30 mg of sample were aliquoted in Eppendorf tubes and homogenized in 400 mL of methanol for 5 min at 25 Hz with a sample disruptor Qiagen TissueLyser II Laboratory Mixer (Qiagen, Valencia, CA, USA). The homogenized mixture was centrifuged for 10 minutes at 10000g (4 °C) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with 500 ng of complementation plasmid (described before) + 25 ng of pCoBlast (Thermo Fischer, Cat. No K5150-01) using Effectene Transfection Reagent (QIAgen, Cat. No 301425). 48 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... the total reaction volume of 25 µl per sample included 12.5 µl TaqMan® qPCR master mix (QuantiTect® Multiplex PCR NoROX Kit (QIAGEN, Hilden, Germany), 1.25 µl of the respective primer/probe mix (Table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA extraction was performed from 25 mg (±2 mg) of arthropod powder with Qiagen Dneasy® Blood & Tissue extraction kit (Qiagen, Hilden – Germany) following the manufacturer’s protocol (see Sire et al ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was extracted from 5 to 10 million cells or 15 to 25 mg of tissue using the MagAttract HMW DNA kit (Qiagen N.V., Venlo, Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 25 mg of whole kidney tissue by manual homogenization using a 25-gauge needle in RLT buffer as described by the manufacturer (Qiagen RNeasy Mini Kit). The purity of RNA was evaluated using a spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... flies were separated in to four pools of 25 and DNA was extracted using a column-based DNA extraction kit (DNeasy Qiagen kit, Cat # 69506). The extracted DNA from each of the four pools of 25 for distinct samples were combined such that the same concentration of DNA was added from each pool (equimolar) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was prepared from day one adult animals raised at 25°C or 20°C by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). endu-2(tm4977 ...
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bioRxiv - Molecular Biology 2020Quote: ... Animals were raised at 25°C for 48 h after hatching and total RNA was purified by using RNeasy® Mini Kit (Qiagen, Venlo, The Netherlands). Microarray and the following data analysis were performed as described before (Qi et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was extracted from ∼25 mg of wing membrane tissue using the Qiagen® DNeasy Blood and Tissue Kits (Qiagen® Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR reactions were performed in triplicates in a total volume of 25 µL with 1X of PCR buffer (including 3mM MgCl2 and 400 μM each dNTP, QIAGEN® Multiplex PCR kit, Qiagen, Germany), 0.5 µM of each primer and 10 ng of template DNA for bulk DNA or 5 µL of template DNA for etDNA ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.
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bioRxiv - Microbiology 2021Quote: ... leaf-disc punches were collected from plants and ground in 250 µL of 10 mM MgCl2 using the TissueLyser II (QIAGEN; 2 cycles of 30 seconds at 25 Hz) and 3-mm zirconium oxide beads (Glen Mills Inc.) ...