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Citations for Qiagen :
1 - 50 of 351 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted in from triplicate flasks (RNeasy, Qiagen) and stored at −80°C before sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL Multiplex Mastermix (Qiagen Multiplex PCR Plus Kit ...
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bioRxiv - Genetics 2019Quote: ... using 25 mL tube extenders (Qiagen) and a vacuum manifold (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... 25 μl HotStarTaq Master Mix (Qiagen) and 10 pmol of each primer ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 µl MgCl2 (25 mM; Qiagen), 0.8 µl Bovine Serum Albumine (BSA ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted from ∼108 parasites per flask using the QIAamp DNA Mini Kit (QIAGEN) for subsequent barcode and whole genome sequencing.
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bioRxiv - Genetics 2021Quote: ... 1.5 μl of 25 mM MgCl2 (Qiagen), 0.3 μl each of 10 μM forward and reverse primers (Table 1) ...
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bioRxiv - Microbiology 2019Quote: ... 25 μl of TopTaq master mix (Qiagen), 0.5 μl of each forward and reverse bar-coded primer (57) ...
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bioRxiv - Cell Biology 2021Quote: ... 25 nM of S1PR1 #1 (Qiagen, #SI00376201) 5’-ATGATCGATCATCTATAGCAA-3’ and 25nM S1PR1 #2 (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... 1 ul of 25 mM MgCl2 (Qiagen) and 1 ul of the primer mix (40 uM of the Forward primer ...
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bioRxiv - Microbiology 2022Quote: HEK293T cells at 50% confluency in a T75 flask were cotransfected using the Effectine Transfection Reagent (Qiagen) with DNA plasmids expressing GFP (gift of Maria Blasi ...
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bioRxiv - Cancer Biology 2019Quote: ... Pathways significantly affected in non-T cell-inflamed or non-T cell-inflamed-enriched mutated genes were identified by IPA® (QIAGEN Inc., Germany).
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bioRxiv - Neuroscience 2021Quote: ... clusterProfiler (v3.12.14) (25) and Ingenuity Pathway Analysis (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: ... and eluted in 25 µl of EB (Qiagen). The resulting sample was quantified using a Quant-iT PicoGreen dsDNA assay (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... 25 ng of XPC and GAPDH (Qiagen, France) cDNAs were then used to launch the qPCR reaction using gene-specific primers along with the Platinum SYBR green qPCR SuperMix-UDG (#Cat.11733038 ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... and 25 pmol primers in a 25 μL reaction volume and subjected to qPCR using Rotor-Gene Q (Qiagen, Venlo, Netherlands). We amplified the tdTomato gene sequence for vector genome quantification using the following primers ...
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bioRxiv - Cancer Biology 2019Quote: ... T cells were sorted directly into 250μl RNAprotect buffer (Qiagen), spun down for 1 minute at 2000 RPM ...
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bioRxiv - Microbiology 2019Quote: ... total RNA from two replicate flasks per time point was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) with on-column DNA digestion using RNase-free DNase (Qiagen) ...
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bioRxiv - Bioengineering 2020Quote: ... The samples were ground by bead beating for 25 sec at 25 Hz using a Tissue Lyser II machine (Qiagen, Hilden, Germany). For CFU-based bacterial enumeration ...
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bioRxiv - Biochemistry 2021Quote: ... 25% PEG 8000 from the PEG suite screen (Qiagen) after 48 hours at 18°C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNase A (25 µg/mL final concentration; Qiagen, 19101) was added ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from ctrl and HD-iNs converted in T25 flasks (200,000 fibroblasts cells plated for conversion per sample) using DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from ∼1000 iDANoids (containing 2000 seeded fibroblasts each) or from a T25 flasks (1.2 × 106 fibroblasts plated) using DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Purified CD4+ T cells were homogenized using the Qiashredder (Qiagen, 79654) and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... DNA was eluted in 25 μL of AVE buffer (Qiagen).
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bioRxiv - Genomics 2021Quote: ... and DNA eluted in 25 µL nuclease-free H2O (Qiagen). When required ...
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bioRxiv - Microbiology 2022Quote: ... 25% (w/v) PEG 1500 (QIAGEN PACT screen, condition 42).
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bioRxiv - Genomics 2023Quote: ... using a TissueLyzer II (QIAGEN, 2min, 25 Hz, 2 times). The samples were incubated at room temperature for 5min ...
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bioRxiv - Neuroscience 2024Quote: ... at 25 Hz for 1.5-3min (Qiagen/Retsch Bead Beater). Following homogenization samples were centrifuged at 16,000 x g for 10 mins at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... at 25 Hz for 1.5-3min (Qiagen/Retsch Bead Beater). Following homogenization samples were centrifuged at 16,000 x g for 10 mins at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Sorted T cells were lysed in Buffer RLT Plus (Qiagen, Hilden, Germany) with 1% β-mercaptoethanol (Millipore-Sigma ...
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bioRxiv - Physiology 2020Quote: ... and Student’s t tests for significance per the manufacturer’s analytical software (Qiagen), with results plotted as fold change showing 95% confidence limits ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... and an undisclosed peptide pool inducing CD8+ T lymphocyte stimulation (Qiagen, 2017); rmsHBHA which tubes contain recombinant M ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from isolated CD8+ T cells (RNeasy kit, Qiagen) and 300 ng of RNA were used for cDNA synthesis (First-Strand Synthesis kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from murine T cells via RNeasy Kit (Qiagen) with DNase digestion (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and CD95+ CD4 T cells using a RNeasy plus mini kit (QIAgen). Isolated RNA sample quality was assessed using a BioAnalyzer RNA pico assay (Agilent Technologies Inc. ...
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bioRxiv - Genetics 2023Quote: ... ~250 × 106 cells from each flask were collected and subjected for genomic DNA extraction using Qiagen’s QIAamp DNA Blood Maxi Kit (Qiagen, Cat # 51194). The integrated gRNAs were amplified (first PCR ...
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bioRxiv - Genetics 2022Quote: ... and sex-matched controls were grown to 80-90% confluency in T75 flasks before total RNA extraction via RNeasy Plus Mini Kit (Qiagen, 74136). Whole blood samples were collected and purified using MagMAX for Stabilized Blood Tubes RNA Isolation Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 25 mL of nuclease-free water (Qiagen). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 25 mL of nuclease-free water (Qiagen). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Ni-NTA agarose resin 25 ml (Qiagen, Germany; cat. no. 30210)
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bioRxiv - Cell Biology 2023Quote: ... to which was added 25 μL of a HiPerFect (Qiagen, 301705) transfection mix (0.75 μL HiPerFect + 24.25 μL serum-free DMEM) ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was isolated from mouse T cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2024Quote: ... total RNA was extracted from T cells by using RNeasy mini kit (QIAGEN) and reverse-transcribed into cDNA with Superiorscript III reverse transcriptase (Enzynomics ...
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bioRxiv - Immunology 2024Quote: T cells were subjected to RNA extraction utilizing the RNeasy Micro Kit (QIAGEN). RNA from the renal cortex was isolated with the NucleoSpin Kit (Macharey-Nagel ...
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bioRxiv - Genomics 2019Quote: ... The final library was eluted in 25 µl Elution Buffer (QIAGEN #19086) and quantitated on an Agilent TapeStation 4200 System using the High Sensitivity D1000 ScreenTape.