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Citations for Qiagen :
51 - 94 of 94 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Colonies were cultured in 2x YT liquid media overnight and plasmids were extracted using QIAprep Spin Miniprep kit (Qiagen).
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bioRxiv - Biophysics 2023Quote: ... Transformants were grown to saturation in 5-8mL of LB media supplemented with ampicillin (100µg/mL) and miniprepped (Qiagen) for Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... They were harvested from culture media for genomic DNA extraction using the Qiagen DNeasy Plant Minikit (QIAGEN Inc., Mississauga, Ontario). A pure culture corresponding to the voucher of cf ...
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bioRxiv - Neuroscience 2019Quote: ... EST plasmids were grown in Luria Bertani (LB) media overnight at 37°C and purified before further use (QIAprep Spin Miniprep Kit, Qiagen). Plasmids were linearized with PauI (New England Biolabs ...
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bioRxiv - Plant Biology 2019Quote: ... RNA for ISO-seq was extracted from cells grown for 3 days in mating inducing media (Sørensen et al., 2014) using the RNeasy Mini kit (Qiagen, USA). Nuclei were isolated from the cell pellets (Raimundo et al. ...
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 nucleic acids were isolated from 300 µl viral transport media using the QIAamp Viral RNA Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were washed with PBS to remove as much media as possible prior to resuspension in 3 mL RNA Protect Bacteria Reagent (Qiagen). After incubating for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen) the next day ...
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bioRxiv - Molecular Biology 2022Quote: ... Extracellular vesicles and RNA were sequentially isolated from the media using the Qiagen exoRNeasy Midi kit (#77144, Qiagen, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... of 0.2-0.3 was pelleted by centrifugation at 7000 x g for 5 minutes and pellets were resuspended in 1mL of ATGN media and incubated with 2 mL of RNAProtect reagent (QIAgen) for 15 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Viral RNA was extracted from 140 μL of cell-free culture media using QIAamp Viral RNA mini kit (Qiagen, Germany), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Swabs were stored without media at - 20°C until DNA was extracted with QiaAmp DNA Mini kit (Qiagen, Germantown, MD). Nasal shedding status of M ...
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bioRxiv - Immunology 2020Quote: ... Phage particles were purified from the culture media using PEG/NaCl and uridine containing single-stranded DNA (dU-ssDNA) extracted using a QIAprep spin M13 kit (Qiagen). Mutagenesis was carried out by annealing degenerated oligonucleotides to the dU-ssDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...
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bioRxiv - Immunology 2022Quote: ... diluted in fresh culture media or left untreated (n = 4) for 6 h prior to harvesting RNA in RLT buffer (Qiagen) with added β-ME ...
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bioRxiv - Pathology 2023Quote: Total RNA from HDLEC treated or not with conditioned media containing APLN were harvested 24h after treatment and isolated using the RNeasy mini kit (Qiagen). DNA digestion was performed using the RNase-Free DNase set (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from cell lysates or the conditioned media using the RNeasy Plus Micro kit (Qiagen, Hilden Germany) or QIAamp Viral RNA kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor samples were transferred in RPMI media supplemented with antibiotics and stored in RNAse later until RNA isolation using the RNeasy Mini Kit (QIAGEN). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Cancer Biology 2023Quote: RNA from 100 µL of serum (35 µL for samples from pregnant dams and 15 µL for samples from P14-16 pups) or 200 µl conditioned media was isolated using the miRNeasy Serum/Plasma Advanced Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... CD4+ T cells were allowed to recover in RPMI-IL2 media for 72 hours post nucleofection before genomic extraction using the Qiagen QiAamp DNA Blood Mini Kit (cat#51104, Qiagen). For VPX co-delivery experiments ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA libraries were created using genomic DNA extracted from 2-week old Col-0 seedlings grown on ½ Murashige and Skoog media supplemented with 1% sucrose with DNeasy Plant Mini kit (Qiagen). Plasmids containing the proteins of interest in the pIX-HALO backbone were ordered from ABRC ...
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bioRxiv - Microbiology 2023Quote: ... in vitro in L-lactate containing minimal media and total RNA was isolated by the TRIzol method and RNeasy minikit (Qiagen). After digestion with TURBO Dnase treatment ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted from 300 µl of viral transport media (VTM, Himedia, India) using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... 50 µM LNA oligo in 100 µL serum free media is mixed with 12 µL HiPerfect transfection reagent (Qiagen, Valencia, CA) and incubated for 20 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... These cells were incubated overnight in MEM-α media free of FBS and supplemented with 1% P/S followed by collection of Supernatant (conditioned media) and total RNA extraction (Cat# 74134, Qiagen, Germany). The resulting samples were stored at −80°C until analyzed further in downstream applications.
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bioRxiv - Cell Biology 2020Quote: ... Spike-in Cel-miR-39 was added to cell conditioned media (CCM) and explanted swine aortic valves for normalization and quality control (219610, QIAGEN, Germany). All reactions were performed in technical triplicates ...
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bioRxiv - Molecular Biology 2021Quote: ... Organ samples collected during necropsy were weighed and homogenized in sterile media using beads in a tissue lyser machine (Qiagen, Germany). The lysate was used for RNA extraction using the MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The next day cells were given fresh media and 24 hours later collected for RNA extraction using the RNeasy Mini Kit (Qiagen #74104) according to manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: ... We then resuspended the viruses in 500 μL of residual media and extracted viral RNA using a QIAamp Viral RNA Mini Kit (Qiagen, 52906). To accurately measure mutation frequencies ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated and the cell pellet was re-suspended in 350 μL RLT buffer (Qiagen RNeasy Mini Kit, No. 74104). Extraction was automated using a QIAcube set to the RNA-RNeasy mini protocol with DNaseI digest and 35 μL DEPC water elution ...
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bioRxiv - Cancer Biology 2023Quote: ... and COMMA1D INSR (-/-) (Clones #87 and #160) cultured in triplicate in COMMA1D media was isolated using the Qiagen RNeasy mini kit (Qiagen # 74104) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
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bioRxiv - Plant Biology 2019Quote: ... For cDNA synthesis RNA of 14 days old seedlings germinated in liquid media under long day conditions was extracted with the RNeasy Mini Kit from Qiagen (Düsseldorf, Germany) and cDNA was synthesized with RevertAid H Minus Reverse Transcriptase from Thermo Fisher Scientific (Karlsruhe ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was isolated from the study strains following overnight growth in media with and without NaHCO3 supplementation using an RNeasy Kit (Qiagen, Valencia, CA) (66) ...
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bioRxiv - Immunology 2022Quote: ... For RNA extraction 140uL of sample (plasma, 1st BAL wash, or swab media) was processed using a Viral RNA mini kit (Qiagen Cat# 52906) and eluted in 50uL RNase Free water ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from cells grown in M9 media containing pyruvate (0.4% w/v) as sole carbon source by column capture (Qiagen RNeasy Mini Kit). Purified RNA was subjected to additional DNase treatment (Turbo™ DNase ...
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bioRxiv - Microbiology 2021Quote: Bacterial genomic DNA was extracted from 1mL overnight cultures grown in BHI media using a Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted from 1 mL overnight cultures grown in LB media using a Qiagen DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from serially diluted and the 4 different media SARS-CoV-2 samples using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, Hilden, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the viral transport media (VTM) containing the nasopharyngeal swabs using the QIAamp® DNA Microbiome Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... bacterial colonies were pooled into LB-ampicillin culture media and amplified followed by plasmid purification with the Plasmid Maxi Kit (Qiagen, Cat. No. 12165). Barcode diversity in the plasmid stock was confirmed via next-generation sequencing (Amplicon-EZ – Azenta Life Sciences) ...