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Citations for Qiagen :
1 - 50 of 94 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... anion exchange chromatography and by Ni-NTA-Agarose (Qiagen #1018244) affinity chromatography as described previously [2,39–41] ...
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bioRxiv - Biophysics 2021Quote: ... DNA plasmids were purified using anion-exchange gravity flow chromatography (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2022Quote: ... The Klenow polymerization reactions were incubated at 37 °C for 1 h and then purified on ion-exchange cartridges (cat. no. 28306; Qiagen). The 194 purified reactions were dissolved in 50 µL of water each and pooled to generate the 3,880 members library (DEL3880 ...
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bioRxiv - Developmental Biology 2023Quote: ... BeWo conditioned media and MEHP (10μM) + BeWo conditioned media) using the RNeasy PLUS Mini Kit from Qiagen (Germantown, MD). RNA extraction followed the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... for the isogenic pair (APOE3 and APOE4) of iMGLs exposed to spheroid conditioned media or unspent neuronal media was achieved with RNeasy Plus Mini Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2023Quote: ... the media was aspirated and RLT buffer (Qiagen) was added to the wells ...
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bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2020Quote: ... media containing cadherin was flowed through Ni-NTA agarose beads (Qiagen) packed on 1 ml Empty Bio-Scale Mini Cartridges (Biorad #7324660) ...
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bioRxiv - Biochemistry 2020Quote: ... Media was removed by filtration using a QIAvac 96 vacuum manifold (Qiagen) and wells were washed two times with 150 μL media ...
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bioRxiv - Genomics 2022Quote: ... exRNA was isolated from cell culture media by two methods: exoRNeasy (Qiagen) and Exosome Total Isolation Chip (ExoTIC) ...
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bioRxiv - Developmental Biology 2024Quote: ... media was removed and cells were lysed with Qiazol (Qiagen, Cat. #79306). RNA extraction was performed according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: RNA was isolated from growth media using QIAmp Viral RNA Mini Kit (Qiagen). Reverse transcription was performed using ProtoScript First Strand cDNA Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... media were aspirated and cells were lysed with RLT plus lysis buffer (Qiagen). Lysates were transferred to 1.5 ml tubes and were fully homogenized using a handheld pestle and mortar ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... The media was then incubated with 1.5 mL of Ni-NTA beads (Qiagen) at 4°C on a rolling bed for 1 hr ...
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were recovered into growth media and then resuspended in RLT lysis buffer (Qiagen). RNA extraction was performed using the Qiagen RNeasy kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Developmental Biology 2021Quote: ... the media was taken off and cells were lysed directly in Qiazol (Qiagen, 79306), followed by RNA preparation.
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bioRxiv - Molecular Biology 2022Quote: ... change fresh media gently and transfect cells according to Effectene Transfection Reagent Kit (QIAGEN).
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bioRxiv - Bioengineering 2023Quote: RNA was extracted from media samples using QIAamp Viral RNA Mini Kit (250) (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and aliquots stored in viral transport media (VTM) and AVL buffer (Qiagen, Milton Keynes, UK) at −80°C until assay.
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bioRxiv - Microbiology 2021Quote: ... Total RNA extraction of the media was performed using Qiamp viral RNA Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Culture media or cells were collected for RNA extraction using an RNA extraction kit (Qiagen). DENV-2 copy number was quantified by one step real time RT-PCR as previously described (16) ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from 140 µl of cultured media using QIAamp RNA extraction kit (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: DNA extraction from media was performed using the DNeasy Blood and Tissue kit (QIAGEN, Hilden, Germany) following manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted from supernatant culture media using the QIAamp 96 Virus QIAcube HT Kit (Qiagen). E-gene expression was determined using the SensiFAST Probe No-Rox One Step Kit (Bioline ...
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bioRxiv - Cell Biology 2021Quote: ... Media and osteoblasts were harvested 3 days later and EVs were collected using Exoeasy kit (Qiagen). Total RNA was extracted using miRNeasy micro (Qiagen).
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bioRxiv - Biophysics 2021Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acid was extracted from cell culture media manually using a QIAamp Viral RNA Mini Kit (QIAGEN) or using NucliSENS easyMAG or EMAG platforms (both BioMérieux) ...
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bioRxiv - Biophysics 2022Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25 mM HEPES ...
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bioRxiv - Genomics 2019Quote: ... Cell-free DNA was extracted from the thawed media using the Qiagen circulating DNA extraction kit (Qiagen) according to the manufacturer’s instructions and quantified using Qubit High Sensitivity DNA quantification kit (Life Technologies) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The RBD was purified from the expression media by metal-affinity chromatography using Ni-NTA beads (Qiagen). The Fabs were purified from the expression media using rProtein A Sepharose Fast Flow beads (GE healthcare) ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed by removing media from culture dishes and adding 200ul of RLT plus buffer (Qiagen) supplemented with 0.5mM 2-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... in serum-free media containing a transfection reagent (HiPerFect Transfection Reagent, catalog no. 301704; Qiagen, Valencia, CA). M- or G-MDSC cultures were subjected to a transient elastase treatment (0.4 U ...
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bioRxiv - Cell Biology 2022Quote: ... total RNA was extracted from EV enriched samples isolated from conditioned media using the miRNeasy Micro kit (QIAGEN), again following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was removed and cells were re-suspended in 30 μL of Turbo Capture Lysis (TCL) buffer (Qiagen) containing 1% beta-mercaptoethanol (BME) ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from roots of 10-day-old plants grown at optimal pH (5.5) or exposed for six-hour to pH 7.5 media using the RNeasy Plant Mini Kit (Qiagen). Three independent biological replicates were performed ...
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bioRxiv - Systems Biology 2024Quote: ... Media was removed and cells were re-suspended in 30 μL of Turbo Capture Lysis (TCL) buffer (Qiagen) containing 1% beta-mercaptoethanol (BME) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from 20 µL of pooled oocyte culture media using the miRNeasy Serum/Plasma kit (Qiagen) in a final elution volume of 10 µL ...
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bioRxiv - Microbiology 2019Quote: Total RNA was extracted from each sample according to the manufacturer’s recommendations using 100 µL of Viral Transport Media as input for the RNeasy Micro Kit (Qiagen). Influenza genomic RNA was subsequently converted into cDNA and amplified (40 cycles of PCR ...
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bioRxiv - Microbiology 2020Quote: ... or 7-recrudescent populations as well as PruQ tachyzoites and alkaline-induced PruQ bradyzoites (pH8.2 media for 72 h) were purified using the RNeasay kit (Qiagen) according to manufacturer protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were grown to approximately 80% confluency in light and heavy media and transfected using Attractene (#301007, Qiagen, Germany) with SBP-empty vector (control cells ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 10-day-old whole seedlings grown on 1/2X MS media containing 1% sucrose and 0.8% agar (Plant RNeasy kit (Qiagen)) ...
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bioRxiv - Microbiology 2022Quote: Viral genomic RNA was purified from 280 μL virus-containing media using the QIAamp Viral RNA Mini Kit (Qiagen) as per the manufacturer’s instructions and eluted in 60 μL nuclease-free water ...
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bioRxiv - Synthetic Biology 2023Quote: ... Colonies were cultured in 2x YT liquid media overnight and plasmids were extracted using QIAprep Spin Miniprep kit (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... Drosophila S2 cells were cultured in Excel media without fetal bovine serum (FBS) and co-transfected with Effectene (Qiagen). The expression of the copper-inducible transgenes was induced by adding CuSO4 (700 µM ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).