Labshake search
Citations for Qiagen :
51 - 100 of 218 citations for Streptococcus Pneumoniae Antigen Native Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... DNA extracts were prepared using Allprep Bacterial DNA/RNA/Protein Kit (Qiagen) following the manufacturer’s instructions and further submitted to Genewiz for bacterial 16S-EZ sequencing (V3 and V4 hypervariable regions ...
-
bioRxiv - Plant Biology 2022Quote: ... the cell extracts were applied to a Ni-NTA spin column (Qiagen) to concentrate the BrBt-SCS proteins and then dialyzed with the same buffer used in the SCS assay ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA extracts were further purified on the GenomicTip G/20 column (Qiagen) by the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... The crude RNA extracts were purified by a RNeasy Mini kit (Qiagen) with a DNase I (Qiagen ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... we extract DNA from toepad tissue mainly following the instructions from Qiagen for animal tissue with the addition of Dithiothreitol (DTT ...
-
bioRxiv - Cell Biology 2019Quote: ... at 23 °C using the previously created pQE60-Rab11 vector (Li et al., 2007; Satoh et al., 2005) and purified in native conditions using Ni-NTA Agarose (QIAGEN, Hilden, Germany). To obtain antisera ...
-
Immune profiling in M. tuberculosis infection enables stratification of patients with active diseasebioRxiv - Immunology 2019Quote: ... 1mL of whole blood was collected directly in QFT Gold tubes (Nil, TB Antigens, Mitogen) (Qiagen) according to manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Cancer Biology 2020Quote: Cell extracts were harvested by direct addition of QIAzol lysis reagent (79306, Qiagen [WERFEN ESPAÑA ...
-
bioRxiv - Microbiology 2019Quote: ... filtered extracts by loading onto a pre-equilibrated Ni-NTA Superflow Cartridge (Qiagen) at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting DNA extracts were purified by using DNeasy Blood & Tissue Kit (Qiagen).
-
bioRxiv - Biophysics 2020Quote: ... This periplasmic extract was then incubated with 5 ml Ni-NTA beads (Qiagen) at 4°C for 15 minutes and the resin slurry was packed on an open chromatography column ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer then eluted in buffer containing 250 mM imidazole ...
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... the soluble extracts were subject to Ni-NTA agarose resin (QIAGEN cat#30210). After passing 20 volumes of washing buffer (50 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... whole cell RNA extract was prepared using the RNeasy Mini kit (Qiagen, 741106) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... from total RNA isolated from whole lung extracts (Qiagen – RNeasy Plus – cat. # 74034) and mRNA expression analysed by a comparative Ct method.
-
bioRxiv - Cell Biology 2023Quote: ... The viral RNA extracts were performed using QIAamp viral RNA kit (Qiagen, UK) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: DNA contents in native/control kidneys (n = 4) and decellularized kidneys (n = 4) were measured using a Qiagen DNeasy Kit (Qiagen, Valencia, CA, USA). The tissues were initially minced and stored overnight at −80°C ...
-
bioRxiv - Physiology 2023Quote: DNA content in native/control kidneys (n = 4) and decellularized kidneys (n = 4) were measured using a Qiagen DNeasy Kit (Qiagen, Valencia, CA, USA). The tissues were initially minced and stored overnight at −80°C ...
-
bioRxiv - Bioengineering 2024Quote: ... we isolated and purified total DNA from native and decellularized small intestine using a DNeasy Blood and Tissue Kit (Qiagen, Cat. No. 69504) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... total RNA extract was prepared from E14Tg2a ESCs using RNeasy Mini Kit (Qiagen, 74104). The first cDNA strand was synthesized using oligo d(T ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA from the whole cell extracts was purified using the RNA-Easy Kit (Qiagen). RNA extracted by TRIzol-Chloroform or the RNA-Easy kit was treated with DNaseI (NEB ...
-
bioRxiv - Evolutionary Biology 2020Quote: RNA extracts in which the virus was detected were DNAse I treated (Qiagen, Germany) for 15 min at room temperature prior to sequencing ...
-
bioRxiv - Molecular Biology 2020Quote: ... The DNAse-digested extracts were then purified using the RNeasy MinElute Cleanup Kit (Qiagen). RNA was reverse transcribed into cDNA using iScript™ Reverse Transcription Supermix (Bio Rad) ...
-
bioRxiv - Microbiology 2021Quote: ... The soluble extracts were applied to a 1 ml of Ni2+- NTA-agarose (Qiagen) column that had been equilibrated with buffer A containing 10 mM imidazole ...
-
bioRxiv - Developmental Biology 2020Quote: ... TRIzol LS Reagent (LifeTechnologies) was used to extract RNA and RNAeasy mini kit (Qiagen) and used to purify RNA ...
-
bioRxiv - Plant Biology 2022Quote: ... CTAB-based method was used to extract DNA and the EpiTect Bisulfite kit (QIAGEN) was used for DNA conversion ...
-
bioRxiv - Microbiology 2023Quote: RNA was extract from 8 x 106 cells using the RNeasy Mini Kit (Qiagen) protocol ...
-
bioRxiv - Microbiology 2023Quote: ... were used to extract and purify the RNA from GAS cells (Qiagen, Valencia, CA). The sequencing library was constructed using an NEBNext rRNA Depeletion Kit (Bacteria ...
-
bioRxiv - Immunology 2021Quote: ... and 12 dpi in order to extract viral RNA (QIAamp® Viral RNA kit, Qiagen).
-
bioRxiv - Neuroscience 2021Quote: ... the hippocampus tissue RNA was extract by using RNeasy® Mini Kit (Qiagen, Hilden, Germany). In brief ...
-
bioRxiv - Microbiology 2019Quote: ... Selected colonies were used to extract plasmid DNA (QIAprep Spin Miniprep Kit; Qiagen, Hilden, Germany). Introduction of mutations were confirmed by Sanger sequencing ...
-
bioRxiv - Bioengineering 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4 °C for 2 h with rotation.
-
bioRxiv - Biochemistry 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4°C for 2 h with rotation ...
-
bioRxiv - Bioengineering 2021Quote: ... to extract RNA based on manufacturer instructions with an on-column DNase I digestion (Qiagen) for minimizing genomic DNA contamination ...
-
bioRxiv - Microbiology 2021Quote: ... The extract was DNAse-treated and purified using a Qiagen RNeasy mini kit (Qiagen; 74104) as per the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: RNA was extract from cells using the RNeasy Mini or Micro Kit (Qiagen, Hilden, Germany) and cDNA was synthesized using iScript cDNA Synthesis Kit (BioRad) ...
-
bioRxiv - Evolutionary Biology 2021Quote: Cytoplasm extracts or nuclei suspensions were mixed with 40 mM DTT-supplemented RLT buffer (Qiagen) and 100% ethanol at 2:7:5 ratio ...
-
bioRxiv - Bioengineering 2022Quote: To extract the RNA a column-based RNA extraction kit purchased from Qiagen (cat# 52904) was utilized ...
-
bioRxiv - Genomics 2019Quote: ... we ran an independent experiment to extract total RNA using a Qiagen kit (Qiagen, #74004) and measured using a BioAnalyzer RNA 6000 Pico kit (Agilent ...
-
bioRxiv - Cell Biology 2020Quote: Five million cells were used to extract total RNA using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... 6x Histagged proteins were purified from the crude extract using Ni-NTA agarose columns (Qiagen) according manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Heart total RNA was extracted from left ventricular extract using the RNeasy Mini Kit (Qiagen) as previously described (50) ...
-
bioRxiv - Microbiology 2023Quote: ... The extract was cleared by ultracentrifugation then nutated with 1mL NiNTA resin (Qiagen, Alameda, CA) at 4◦C for two hours ...
-
bioRxiv - Neuroscience 2023Quote: ... was used to extract RNA from cells that were lysed with QIAzol® reagent (Qiagen). The extraction was conducted according to the manufacturer’s protocol ...
-
bioRxiv - Physiology 2024Quote: Total RNA was isolated from fat tissue extracts or EMSCs using RNeasy mini-Kit (Qiagen). Reverse transcription was performed using 500 ng of total RNA and iScriptTM reverse transcription super mix (Bio-Rad ...
-
bioRxiv - Genomics 2020Quote: ... DNA extracts from sorted cells were prepared using with DNeasy Blood and Tissue Kit (Qiagen, 69504) and single-end 100-base sequencing libraries prepared using TruSeq kit (Illumina ...
-
bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated using RNeasy minicolumns from cell extracts prepared with QiaShredder (Qiagen RNeasy Kit). The concentration and purity of RNA were assessed by a NanoDrop DM-1000 spectrophotometer (NanoDrop Technologies) ...
-
bioRxiv - Cancer Biology 2019Quote: ... 16 mg of total cell extract (TCE) were incubated with 320 μL of NiNTA beads (Qiagen) at 4 °C ...