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Citations for Qiagen :
301 - 350 of 1207 citations for Respiratory Syncytial Virus A Glycoprotein G Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was finally extracted using Genomic Tip G-500 columns (Qiagen) and cleaned using the PowerClean DNA Clean-Up kit (MoBio Labs) ...
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bioRxiv - Genomics 2021Quote: ... 56 μL of amplification mix (REPLI-g Single Cell Kit, Qiagen) that contained alpha-thio-ddNTPs (Trilink Bio Technologies ...
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bioRxiv - Genetics 2019Quote: ... and DNA was extracted using Genomic Tip G-500 columns (Qiagen) and cleaned using the PowerClean DNA Clean-Up kit (MoBio Labs) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... whole genome amplification was performed using REPLI-g Mini kit (Qiagen) due to the low concentrations of gDNA ...
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bioRxiv - Genomics 2021Quote: ... HMW gDNA was captured by magnetic particles (Qiagen MagAttract Suspension G), and then the magnetic particles with HMW gDNA was washed in wash buffer and eluted in EB Buffer (10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... A nonmethylated control was prepared using REPLI-g Mini kit (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... Control unmethylated DNA was prepared using a Repli-g kit (QIAGEN). C ...
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bioRxiv - Neuroscience 2022Quote: ... using Qiagen Genomic-tip 100/G HMW Kit (Qiagen, Hilden, Germany), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: We extracted DNA using the Qiagen Genomic Tip 20/G (Qiagen) and followed the manufacturer’s protocol “Preparation Gram-negative and some Gram-positive Bacterial Samples” ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the QIAGEN Genomic-tip 20/G standard protocol (Qiagen, UK). All samples were sequenced at the University of Liverpool from Illumina TruSeq Nano libraries with 350bp inserts using 125bp paired-end reads on an Illumina HiSeq2500 platform ...
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bioRxiv - Microbiology 2019Quote: ... pylori-infected mice using a genomic-tip 20/G kit (Qiagen, catalog no ...
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bioRxiv - Genomics 2021Quote: ... 4 μL of Buffer D2 (REPLI-g Single Cell Kit, Qiagen) and 1 μL of 500 μM exonuclease-resistant random primer were then added to the lysed cells to denature the DNA prior to vortexing ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was purified using Qiagen Genomic-tip 20/G (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... followed by DNA extraction using Genomic Tip G-500 columns (Qiagen) and cleaning with the PowerClean DNA Clean-Up kit (MoBio Labs) ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... 20 μL REPLI-g sc DNA Polymerase (Qiagen, catalog no. 150345), 30 μL 10 x Eva green (biotium ...
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bioRxiv - Evolutionary Biology 2022Quote: ... genomic DNA was directly amplified using the Repli-G kit (Qiagen), and then sequenced with Illumina HiSeq from TruSeq gDNA libraries by GenomeQuebec ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 g roots using RNeasy Plant Mini Kit (QIAGEN, Canada). Extracted RNA was treated with DNAse (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used a Repli-G whole- genome amplification kit (Qiagen, Inc.) to increase the amount of DNA prior to library preparation ...
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bioRxiv - Microbiology 2024Quote: Plasmid DNA was amplified using REPLI-g® Midi Kit (QIAGEN), according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2019Quote: ... Short CAP was expressed and His tag purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen, Hilden, Germany). The shortCAP recombinant protein was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Developmental Biology 2020Quote: ... mUHRF1 C-terminally tagged with GFP- and 6xHis-tag was expressed in HEK 293T cells and then purified using Qiagen Ni-NTA beads (Qiagen #30230). Recombinant mDPPA3 WT and 1-60 were purified as described above ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatants containing TtgR and SmtB were purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The eluted fractions from the FPLC (for TetR ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clarified supernatant containing mRFP1 was then purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The elution from the gravity column was concentrated and buffer exchanged (25 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and Vero E6 cells infected with 2 patient virus isolates was converted to cDNA using reverse transcriptase from qiaseq SARS-CoV-2 primer pool (Qiagen kit, cat no. 333896). APOBEC3b (sense ...
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bioRxiv - Neuroscience 2022Quote: RNA from frozen FC area 8 was extracted following the supplier’s instructions (RNeasy Mini Kit, Qiagen® GmbH, Hilden, Germany). RNA integrity and 28S/18S ratios were determined with the Agilent Bioanalyzer (Agilent Technologies Inc ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and whole-genome amplified using a REPLI-g Single Cell kit (Qiagen) before sequencing on either Illumina NextSeq500 at the Department of Biochemistry ...
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bioRxiv - Genomics 2020Quote: ... total gDNA was obtained from tissue using Genomic-tip 100/G (Qiagen) for the spotty (Notolabrus celidotus) ...
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bioRxiv - Genomics 2019Quote: DNA was isolated using Qiagen Genomic-tip 100/G (Qiagen, Hilden Germany) according to the instructions of the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... D1 buffer from REPLI-g single cell kit (Qiagen, catalog no. 150343) was added to denature the DNA ...
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bioRxiv - Microbiology 2019Quote: ... DNA was then extracted using the Genomic-Tip G/500 kit (Qiagen) following the manufacturers protocol for Gram negative bacteria and eluted into 5 mM Tris pH 7.5 0.5 mM EDTA buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... WGA was conducted using the REPLI-g Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... multiple displacement amplification (MDA) reaction mix (REPLI-g Single Cell Kit, QIAGEN) was added to the lysate and incubated at 30 °C for 3 hours ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was extracted using a ‘Genomic-tip 20/G’ kit (Qiagen) and sequenced with 150 bp paired-end ...
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bioRxiv - Microbiology 2023Quote: ... Amplified DNA libraries were prepared with the Repli-G mini kit (Qiagen) and LSK114 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA extracts were further purified on the GenomicTip G/20 column (Qiagen) by the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... the supernatant was loaded on equilibrated Genomic-tip 20/G column (QIAGEN) and washed and eluted according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA was extracted using the Genomic tip 20/G kit (Qiagen) per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP-positive cells were sorted into REPLI-g cell storage buffer (Qiagen).
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bioRxiv - Genomics 2024Quote: Genomic DNA was isolated using the Genomic-tip 100/G columns (Qiagen) according to “Protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... followed by two purification steps with Genomic tip 500/G (QIAGEN, Germany). To generate a high-quality genome assembly ...
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bioRxiv - Microbiology 2024Quote: As outlined by the REPLI-g cell WGA & WTA handbook from Qiagen, 8 µL of lysis buffer was added to 13 µL of the final RNA fraction of interest and was incubated at 24 °C for 5 minutes followed by 95 °C for 3 mins and cooled down at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid was extracted from 200 μl of each sample using the QIAamp MiniElute™ Virus Spin Kit nucleic acid (for DNA and RNA) according to the manufacturer (QIAgen Canada, Toronto, ON, Canada). DNA samples were stored at −80°C and assayed in duplicate by qPCR ...
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bioRxiv - Bioengineering 2020Quote: ... The RBD wildtype and mutant proteins of SARS-CoV-2 spike with 10×his tag at N terminal were expressed in HEK 293 and purified with Ni-NTA affinity columns (Qiagen, Hilden, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis of 100 ng total RNA was performed with miRCURY LNA RT Kit (10 µl volume reaction) which adds a 5’ universal tag of a poly(A) tail to mature miRNA templates (QIAGEN, Germantown, MD). cDNA template was diluted 1:10 ...
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bioRxiv - Microbiology 2020Quote: ... bearing a C-terminal histidine tag (ACRO Biosystems, Newark, NJ) was coated at 2 μg/ml on a Ni-NTA plate (Qiagen, Valencia, CA). After washing and blocking ...