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Citations for Qiagen :
551 - 600 of 1207 citations for Respiratory Syncytial Virus A Glycoprotein G Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 2 g powdered clay samples using the PowerMax Soil DNA Isolation Kit (Qiagen, Germany) and modifications to the manufacturer’s instructions previously validated (22) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer A supplemented with 200 mM imidazole in gravity flow ...
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bioRxiv - Immunology 2022Quote: Bacterial genomic DNA was isolated using the Blood and Cell Culture DNA kit and 500/G genomic tips (Qiagen) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting with buffer A supplemented with 250 mM imidazole in gravity flow ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B supplemented with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... cleared by centrifugation (20 min, 20,000 × g, 4°C) and the clarified lysate was purified on Ni-NTA resin (QIAGEN) eluting in buffer C supplemented with 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and clarified lysate was purified on Ni-NTA agarose resin (QIAGEN) eluted with buffer A supplemented with 0.5 mM TCEP and 200 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and cleared lysate was subjected to Ni-NTA resin (QIAGEN), washed and protein eluted with buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Systems Biology 2022Quote: ... Ten millilitres of culture were pelleted by immediate centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins and bacterial membranes were separated by centrifugation (30 min, at 15,000 ×g) and the supernatant was applied to Ni-NTA beads (Qiagen) for 2h ...
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bioRxiv - Microbiology 2020Quote: ... The homogenates were clarified by centrifugation at 9,000 × g for 5 min and RNA was extracted using RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... Six replicate 0.35 g extractions were combined and re-purified with the MO BIO PowerClean Pro kit (QIAGEN, Germany) and eluted in 50 mL of 10 mM Tris buffer.
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from the 0.25 g of soil from the resulting pellet using the DNeasy PowerSoil Kit (Qiagen). DNA extractions were verified by gel electrophoresis in a 1% agar gel.
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The mitochondrial genome of the red icefish (Channichthys rugosus) casts doubt on its species statusbioRxiv - Zoology 2022Quote: ... the lysates were centrifuged at 17,000 x g for 5 minutes and 300 μL supernatant mixed with 3000 μL Buffer PB (Qiagen). The mixtures were loaded onto Minelute silica columns (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... Debris was removed by centrifugation (20,000×g, 30 min, 4°C) and the supernatant was incubated with Ni-NTA resin (Qiagen) for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA from Cas9 control and OCT4-targeted was isolated using the REPLI-g Single Cell Kit (QIAGEN, 150343). DNA samples isolated for cytogenetic analysis were amplified with the SurePlex Kit (Rubicon Genomics) ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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Germline Selection by Meiosis Defends the Transmission of defective Mitochondria with mtDNA variantsbioRxiv - Cell Biology 2020Quote: ... Then each PB1 sample was amplified with whole genome amplification (WGA) using REPLI-g Single Cell Kit (QIAGEN, 150345). For each oocyte sample ...
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bioRxiv - Microbiology 2020Quote: ... and IMX2137 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 g of meat or processed food powder was extracted and purified using DNeasy Mericon Food Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was isolated by centrifugation for 30 min at 20,000 ×g and then incubated with a Ni2+-NTA column (QIAGEN) for 5h ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris and unlysed cells were pelleted at 48,000 × g and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The solution was further cleared at 20,000 × g at 4 °C and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
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Phylogenomics of a new fungal phylum reveals multiple waves of reductive evolution across HolomycotabioRxiv - Evolutionary Biology 2021Quote: ... Whole genome amplification (WGA) was carried out by multiple displacement amplification with the single-cell REPLI-g kit (QIAGEN). DNA amplification was quantified using a Qubit fluorometer (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: Soil DNA was extracted from 0.3 g of well-mixed soil for each sample using the DNeasy PowerLyzer PowerSoil Kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell debris was cleared by centrifugation for 30 min at 10,000 x g and passed over Ni-NTA agarose resin (Qiagen) pre-equilibrated with buffer A at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Samples were spun at 5000 x g for 10 minutes and supernatant was discarded before resuspension in 1.5 mL QIAzol reagent (Qiagen). Screw-cap tubes were prepared for each sample by adding ∼250 µL of sterile beads and the QIAzol suspension mixture was added and incubated for 5 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were collected by centrifuging the plates for 10 min at 1,200 x g and the plasmids’ DNA was purified using the QIAGEN Plasmid Plus 96 Miniprep Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Genomics 2022Quote: ... High molecular weight DNA was extracted from liver tissue using a Genomic-tip 20/G DNA kit (QIAGEN, 10223).
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bioRxiv - Genomics 2022Quote: Long fragment DNA extraction from sub-cultured strains was performed using the Qiagen Genomic tip 100/G kit (Qiagen). Quality and fragment length assessments were measured with the Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The aqueous phase was separated by centrifugation at 13,000 g at 4°C and RNA was purified using the RNeasy Mini Kit (Qiagen) with on-column DNAse digestion ...
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bioRxiv - Immunology 2022Quote: ... Lysate was cleared from debris by centrifugation at 30,000 g and 4°C for 30 min before being loaded onto a Ni-NTA Superflow cartridge (Qiagen) using an Äkta Explorer chromatography system (GE Healthcare) ...
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bioRxiv - Physiology 2022Quote: ... Lysates were centrifuged at 100,000 x g for 1 h and the supernatant were affinity purified on Ni-NTA resin (Qiagen) by batch binding for 30 min-1h ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was recovered by centrifugation at 40 000 × g in a JLA25.50 rotor and put through a gravity flow column with 5 ml of NiNTA Agarose (Qiagen). Bound fraction was washed in Buffer B (300 mM NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... The lysed solution was centrifuged at 12,000 × g for 30 min and the supernatant was incubated with Ni-NTA agarose (Qiagen) at 4 □ for 3 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 50 μg of gDNA were digested with KpnI and passed through a QIAGEN Genomic-tip 20/G column (QIAGEN) to enrich for replication intermediates ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by centrifugation (2500 x g, 3.5 min) and washed with 1 mL of RNAse-free water (Qiagen) before being spun down (10k rpm ...
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bioRxiv - Microbiology 2023Quote: ... the lungs were removed and total RNA was isolated by homogenising 0.1 g of lung tissue in a TissueLyser LT (Qiagen) then incubating with TRIzol™ (Invitrogen™ ...
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bioRxiv - Microbiology 2023Quote: ... 50000 x g) and the supernatant was filtered and loaded on a column containing Ni2_NTA (nitrilotriacetic) agarose (Qiagen, Netherlands). The column was equilibrated with wash buffer and the bound protein washed ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were harvested through centrifugation at 7025 x g and re-suspended in non-denaturing lysis buffer (Qiagen) containing 10 mM Tris ...
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bioRxiv - Microbiology 2023Quote: Total microbial DNA was isolated from 0.2–0.5 g faecal material using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany) using the QIAcube (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 24,000 g for 30 min and applied to a 5 mL column bed of Ni-NTA resin (Qiagen) for purification by IMAC ...
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bioRxiv - Biochemistry 2023Quote: ... The sonicate was centrifuged at 38,000 × g for 30 min and the supernatant was loaded on a Ni-NTA agarose column (2.5 × 5 cm, Qiagen) pre-equilibrated with buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysate was cleared by centrifugation at 100,000 × g for 30 min at 4 ⁰C and then incubated with Ni2+-NTA agarose beads (Qiagen) for 30 min at 4⁰C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (3,000 g, 15 min, 4°C) and total RNA was isolated using the miRNeasy Mini Kit (QIAGEN) in combination with the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Genomics 2023Quote: Genomic DNA from each cell was amplified using the Repli-G Advanced DNA Single-Cell kit and protocol (QIAGEN) for amplifying purified genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... 0.25 g of homogenized tissue were transferred to the beating tube of DNeasy®PowerSoil®Kit (Qiagen, Germany) following the manufacturer’s instructions ...