Labshake search
Citations for Qiagen :
701 - 750 of 1207 citations for Respiratory Syncytial Virus A Glycoprotein G Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: Cells were pelleted by centrifugation after isolation (500 g for 10 min) and subsequently lysed for RNA isolation using RNeasy Micro Kit (Qiagen). For some assays ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted from entire individuals with a salting out method57 and amplified using the REPLI-g Mini Kit (QIAGEN) according to the manufacturer’s protocol with volume modifications (Supplementary Tab ...
-
bioRxiv - Cancer Biology 2022Quote: ... Remaining circular DNA was amplified by Multiple Displacement Amplification using φ29 DNA polymerase and random hexamer primers using the Qiagen REPLI-g Mini Kit (Qiagen). Magnetic bead-based purification was used to remove the polymerase and primers ...
-
bioRxiv - Genomics 2022Quote: ... the genomic DNA of the Nichi01 strain was isolated from the silk glands of two male fifth-instar larvae using Genomic-tips 100/g and Genomic DNA Buffer Set (QIAGEN). Long-read sequencing for genome assembly was performed using continuous long-read (CLR ...
-
bioRxiv - Genomics 2022Quote: ... 32 μl Master Mix containing 30 μl MDA reaction buffer and 2 μl Phi29 polymerase (REPLI-g UltraFast Mini Kit; Qiagen) were added ...
-
bioRxiv - Microbiology 2022Quote: ... The ssDNA genome of the ICBM5 phage was converted to dsDNA by using the REPLI-g Mini kit (Qiagen, Netherlands) and then sequenced with Illumina (paired-end technology 2×300 bp ...
-
bioRxiv - Microbiology 2022Quote: ... the beads were suspended in Buffer D2 (denaturation buffer) and subjected to MDA reaction using a REPLI-g Single Cell Kit (QIAGEN).
-
bioRxiv - Microbiology 2022Quote: Cyanobacterial cells were collected by centrifugation of liquid cultures at 10000 g for 5 min and DNA was extracted from cell pellets with the DNeasy PowerBiofilm kit (Qiagen) following manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Total DNA and RNA were extracted from ~1.5 g of rhizosphere soil using the RNA PowerSoil Kit with the DNA elution kit (Qiagen, Germany). DNA and RNA were extracted using ~0.03 g of roots using the DNeasy Plant DNA Extraction Kit ...
-
bioRxiv - Neuroscience 2023Quote: ... freshly sorted cells were pelleted at 500 g for 10 minutes at 4° and then resuspended in RLT Plus buffer (Qiagen). Cells were vortexed and frozen for at least one day at −80° before being thawed on ice and processed for RNA using an RNeasy Mini Kit (Qiagen) ...
-
bioRxiv - Microbiology 2023Quote: Total DNA for SCS_HM2 sediments (∼0.5 g) were extracted using the PowerSoil DNA Isolation Kit (Catalog No. 12888-50, Qiagen, Germantown, MD) following the user’s instructions ...
-
bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted from stationary broth culture using genomic DNA-Tip 100/G system and buffer set (Qiagen) following the manufacturer’s instruction ...
-
bioRxiv - Evolutionary Biology 2023Quote: High-molecular weight DNA was extracted from podetia of Noboribetsu samples using buffers in DNeasy Blood & Tissue Kit and Genomic-tip 20/G (QIAGEN) with lysis time extended to 5 hours ...
-
bioRxiv - Genomics 2023Quote: ... A second DNA library was produced using 2.5 µL native DNA from the same sample after whole genome amplification procedure following the REPLI-G® (Qiagen) manufacturer’s recommendation.
-
bioRxiv - Molecular Biology 2023Quote: ... MDA controls were performed employing a random-primers-based MDA (RP-MDA) kit as random-primer-based method: Repli-g Mini Kit from QIAGEN; and a TruePrime-based-MDA (PrimPol-MDA ...
-
bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
-
bioRxiv - Molecular Biology 2023Quote: ... Approximately 1 μg of small RNAs in 8.5 μl were incubated with 2 μl of QIAseq FastSelect –rRNA Yeast Kit (Qiagen, #334215) at 75°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then collected by centrifugation at 4000 x g for 20 minutes and purified using Ni–NTA–agarose (Qiagen) using standard conditions ...
-
bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
-
bioRxiv - Microbiology 2023Quote: ... 2 ml of the culture was harvested and centrifuged at 11,000 × g for 10 minutes where the cell pellet was used for genome extraction with the DNeasy Blood & Tissue Kit (Qiagen) protocols ...
-
bioRxiv - Cancer Biology 2023Quote: ... by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
-
bioRxiv - Genomics 2023Quote: ... 1µM Template-Switching Oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” prefixes a ribonucleic acid base and “+” prefixes a locked nucleic acid base, Qiagen) then incubated using a ThermoMixer C with the following conditions ...
-
bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
-
bioRxiv - Biophysics 2023Quote: ... The solubilized supernatant was collected by another round of ultracentrifugation (200,000 × g, 1 h) and applied to a Strep-Tactin resin column (Qiagen, USA) equilibrated with buffer A containing 0.025% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: ... the lysate was spun out of the Feather cartridge at 4000x g and added 100 µL of Ni-NTA agarose resin (Qiagen) to capture HIS-tagged proteins ...
-
bioRxiv - Neuroscience 2023Quote: ... The remaining cells were pelleted at 1000 x g for 10 minutes at 4°C and lysed in 350 µL of Buffer RLT (Qiagen) supplemented with 3.5 µL of 2-β mercaptoethanol (#444203 ...
-
bioRxiv - Microbiology 2023Quote: ... an unbiased amplification method was employed by amplifying the purified DNA at 30°C for 8 hours using a REPLI-g Single Cell kit (Qiagen). The amplified DNA was purified using AMPure XP magnetic beads (Beckman Coulter ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1.5 h in 50 mL conical tubes ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were selected in puromycin (1 μg/ml) the next day and after 4 days genomic DNA was extracted using DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sonicated to an average size of 500 bp using S2 Focused-ultrasonicator (Covaris ...
-
bioRxiv - Microbiology 2023Quote: ... DNA for genomic sequencing was further purified using a Qiagen Genomic-tip 20/G kit as described in the manufacturer’s protocol (Qiagen, Germany). Cells from cultures were concentrated at 10,000 × g for 15 min before DNA extraction ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting total cell lysate was clarified by centrifugation (30,000 g for 25 min) and the supernatant fraction was applied to a 4 mL packed Ni-NTA resin (Qiagen) and gently rocked at 4 °C for 1 h in 50 mL conical tubes ...
-
bioRxiv - Microbiology 2023Quote: cDNA was generated from 1 µg of RNA using qScript cDNA SuperMix (QuantaBio) and the cDNA was purified using a QIAquick PCR Purification Kit (Qiagen). 2 µL of cDNA was used in a PCR reaction with primers specific to EF_B0058 and EF_B0059 (Table S1) ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged for 30 min at 38,000 g and 4°C and cleared supernatants loaded onto Ni-NTA columns with 0.5 mL bed volume (1018244 Qiagen, Germany). The columns were washed sequentially with 3 column volumes (CV ...
-
bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... GFP+ and GFP- nuclei were sorted using a BD AriaFACS III (University of Washington Pathology Flow Cytometry Core) into a PCR tube strip containing 3 µL of REPLI-g Advanced Single Cell Storage buffer (Qiagen). Whole genome amplification (WGA ...
-
bioRxiv - Immunology 2023Quote: ... The resulting cell suspension was concentrated by centrifugation (7 min, 350 g) and lysed in RLT buffer(Qiagen, cat. 79216) 0.1-0.5 *10^6 lymphocytes /sample density ...
-
bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... The bacterial cells were harvested by centrifugation and the DNA extraction was performed by the used of Genomic-tip 100/G column (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: The DNA was extracted from the FACS sorted cells and whole genomes were amplified using multiple displacement amplification technique (MDA) with the REPLI-g Single Cell kit (Qiagen) by following the manufacturer instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was centrifuged again at 5,000 x g for 20 minutes at 4°C and equilibrated for 1 hour in 3 mL of Ni-NTA resin (Qiagen) that was pre-equilibrated in the extraction buffer ...
-
bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from the purified oocysts using the Qiagen DNA Mini Kit and amplified using the REPLI-g Midi Kit (QIAGEN). WGA products were purified using the EasyPure PCR Purification Kit (TransGen Biotech ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... alphenor females that were homozygous for the h or H alleles using the QIAgen GenomicTip G-100 kit (QIAgen, USA). Extractions followed the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Cytosolic fractions were prepared by centrifugation at 10,000 × g for 30 min at 4°C and DNA was isolated from them using the DNeasy Blood & Tissue kit (Qiagen). The copy number of mitochondrial DNA encoding 16S RNA (RNR2 ...
-
bioRxiv - Microbiology 2024Quote: ... Lysates were centrifuged for 30 min at 30000 x g in 4 °C and the resulting supernatants were gently mixed with Ni-NTA Agarose beads (Qiagen) pre-equilibrated with lysis buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
-
bioRxiv - Molecular Biology 2024Quote: ... whole genome amplifications were performed on individual embryos by using REPLI-g Advanced DNA Single Cell Kit (catalog no. 150363, Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell lines and mouse tails using DNeasy Blood & Tissue kit (Qiagen). Exome libraries were prepared by the Advanced Sequencing Facility at the Crick and sequenced using an Illumina HiSeq 4000 using 100 base pair paired-end reads ...
-
bioRxiv - Physiology 2019Quote: ... Total RNA from mouse islets was extracted using RNeasy microkit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2020Quote: RNA was isolated from mouse liver using the RNeasy Kit (Qiagen). RNA degradation was monitored using a BioAnalyzer (Agilent) ...
-
bioRxiv - Physiology 2020Quote: ... mouse placenta tissue was homogenized using the Qiagen TissueLyser II (Qiagen) with 5 mm beads in RLT buffer (Qiagen ...