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Citations for Qiagen :
151 - 200 of 842 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and compact bead mill (TissueLyser LT, Qiagen). Colon tissue was placed in TRIsure reagent during homogenization ...
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bioRxiv - Microbiology 2019Quote: ... The resulting 6xHis-tagged expression constructs were utilized to generate recombinant enzymes that were purified via Ni-NTA agarose (Qiagen). IDS enzyme assays were carried out in triplicate as previously described [45] ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose, Qiagen) following the purification hybrid method from the ProBond purification system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged PSK1 or RGF1 precursors were purified from bacterial extracts by metal chelate affinity chromatography on NiNTA Agarose (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The suspensions were lysed with TissueLyser LT (Qiagen), followed centrifuged at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissue were homogenized immediately using TissueLyser LT (Qiagen), for 10 minutes at 50 Hz and cell suspension were vortexed for 3 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and then vortexed using the TissueLyser LT (Qiagen) for three minutes at 45 oscillation per second ...
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bioRxiv - Cell Biology 2021Quote: Tissue biopsies were homogenized using TissueLyser LT (Qiagen) before RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... tissue was homogenized using the TissueLyser LT (Qiagen) for 5 min at 50 Hz ...
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bioRxiv - Microbiology 2020Quote: ... then mechanically lysed using a TissueLyser LT (Qiagen) with Lysing Matrix B tubes (MP Biomedicals ...
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bioRxiv - Molecular Biology 2021Quote: ... Homogenization was performed with a TissueLyser LT (Qiagen) for 2 minutes at 50 Hz ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenization was performed with a TissueLyser LT (Qiagen) for 2 minutes at 50 Hz in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 Hz at room temperature (TissueLyser LT, Qiagen). Homogenized tissue lysates were further processed according to the TRIsure manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... The samples were homogenized using TissueLyser LT (Qiagen). The protein concentration was measured in triplicate using Pierce BCA Protein assay (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using a TissueLyser LT (Qiagen) and RNEasy MiniKit (Qiagen 74106) ...
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bioRxiv - Developmental Biology 2023Quote: ... using a bead beater (TissueLyser LT, Qiagen, 69980) at 50 Hz for 5min ...
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bioRxiv - Biochemistry 2022Quote: ... Tissue pieces were disrupted with TissueLyser LT (Qiagen) at 50 Hz (1 min ‘on’ ...
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bioRxiv - Neuroscience 2023Quote: ... with initial homogenization on a TissueLyser LT (Qiagen). RNA concentrations were determined using the QubitTM RNA Broad Range Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: Tissue biopsies were homogenized using TissueLyser LT (Qiagen) before RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) and expression of the His-tagged Cas proteins was performed following the instruction of the protein purification kit (Qiagen, Valencia, CA, USA). Single colonies of transformed cells were cultivated overnight ...
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bioRxiv - Cell Biology 2021Quote: The GST fusion proteins were expressed in bacteria (E. coli, BL21) and purified by glutathione-Sepharose (Cat No. 27-4574-01, Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Bacterial clones on selection plates were scraped and pooled to extract recombinant plasmids containing the recipient barcodes and DNA blocks (donor barcodes, oligonucleotides, and E. coli ORFs) using Plasmid Plus Mini Kit (QIAGEN). The pooling capacity is determined by the number of unique positioning barcodes ...
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bioRxiv - Immunology 2021Quote: ... and HRP-anti-His (Penta-His Ab, Qiagen) was used in the detection step ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... with a steel bead in a TissueLysser LT (Qiagen) as described above ...
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bioRxiv - Neuroscience 2022Quote: ... with a steel bead in a TissueLyser LT (Qiagen) for 3 min at 50 Hz ...
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bioRxiv - Cell Biology 2022Quote: ... and liver was extracted using the TissueLyser LT (Qiagen) and the RNeasy® Lipid Tissue Mini Kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and homogenized by Tissue Lyser LT (Qiagen, Madrid, Spain). After a 10-min centrifugation (12,000 × g and 4 °C) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were lysed using Tissuelyzer LT bead mill (Qiagen), and DNA was purified using the MO BIO PowerSoil kit according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Halt Protease Inhibitor Cocktail (Thermo Scientific)] with a steel bead in a TissueLyser LT (Qiagen) for 5 min at 50 Hz ...
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bioRxiv - Neuroscience 2020Quote: ... Halt Protease Inhibitor Cocktail (Thermo Scientific)] with a steel bead in a TissueLyser LT (Qiagen) for 5 min at 50 Hz at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... through mechanical disruption using Qiagen TissueLyser LT (Qiagen, UK), at 4oC (40Hz ...
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bioRxiv - Immunology 2023Quote: ... Lung tissue was homogenized using a TissueLyser LT (Qiagen), and total RNA was extracted using RNeasy Mini kit including DNA removal (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: TAs were lysed using a TissueLyser LT (Qiagen, 85600) with 5mm (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... These tubes were placed in the TissueLyser LT (QIAGEN) for next 10 mins at 40 1/s ...
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bioRxiv - Immunology 2022Quote: ... and kidney were homogenized in the TissueLyser LT (Qiagen). After centrifuging at high speed (14000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... and homogenized in TissueLyser LT (Qiagen, Hilden, NRW, Germany). Each RNA sample (1 µg ...
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bioRxiv - Microbiology 2024Quote: ... and cells were disrupted with a TissueLyse LT (Qiagen) for 5 mins at 50Hz ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 min at 40 Hz using TissueLyser LT (Qiagen). 500 µl PBS-tween (0.01% ...
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bioRxiv - Physiology 2022Quote: ... Samples were homogenized using a TissueLyser LT (Qiagen, Hilden, Germany) set to 50 Hz for 5 min (with a rotor pre-chilled to −20°C) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Tissues were homogenized using a TissueLyser LT (Qiagen, Venlo, Netherlands), and total RNA was extracted using the RNeasy Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... the collected tissues were homogenised using the TissueLyser LT (Qiagen) in 600 μl of prepared RLT lysis buffer (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by mechanical disruption of the tissue (TissueLyser LT, Qiagen). Samples were centrifuged at 5000RPM for 10 minutes ...
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bioRxiv - Physiology 2019Quote: ... with a TissueLyser LT (Qiagen N.V. #69980, Venlo, The Netherlands). The lysate centrifuged at 12,000g (15 min at 4°C ...
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bioRxiv - Zoology 2020Quote: ... Each pool was homogenized with TissueLyser LT (Qiagen, Hilden, Germany) in 500 μl of 0.15 M NaCl solution ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with homogenization conducted using a Tissuelyser LT (Qiagen, Hilden, Germany). Concentration and purity of samples were determined by spectrophotometric analysis (Implen nanofotometer ...
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bioRxiv - Genomics 2021Quote: ... in which samples were lysed using the TissueLyser LT (QIAGEN) (30 second oscillations/3 minutes at 30Hz) ...
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bioRxiv - Physiology 2022Quote: ... using the Qiagen TissueLyser LT Bead Mill (QIAGEN, Venlo, Netherlands). Total RNA from each sample was extracted and reverse-transcribed to cDNA with PrimeScript RT reagent kit (Takara Clontech ...