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Citations for Qiagen :
451 - 500 of 842 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... the samples were thawed and physically disrupted using a beadmill (1 min max speed) using a TissueLyser LT (Qiagen, Hilden, DE) and then extracted with phenol-chloroform including an extra chloroform step and several RNA washing steps ...
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bioRxiv - Physiology 2024Quote: 50 mg of mouse spleens were homogenized in 500 μl of the same lysis buffer using the TissueLyser LT (Qiagen, #85600) (50 sec for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... coli using the QIAprep Spin Miniprep kit (Qiagen, #27104). Genomic DNA (gDNA ...
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bioRxiv - Microbiology 2020Quote: ... coli by affinity chromatography using Ni-NTA agarose (Qiagen) according to the manufacturer’s instructions (Ruc-His6 was purified under urea denaturing conditions) ...
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bioRxiv - Microbiology 2020Quote: ... coli using the QIAprep Miniprep kit (QIAGEN, Germantown, MD) and confirmed by PCR to contain the construct again using the CjNC110F2 and CjNC110R2 primers.
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bioRxiv - Microbiology 2022Quote: ... coli via QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) prior to restriction enzyme digestion ...
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bioRxiv - Microbiology 2021Quote: ... coli with the Qiagen mini plasmid preparation kit (Qiagen). Pneumococcal DNA was isolated by phenol/chloroform extraction and ethanol precipitation from an exponentially growing culture in ThyB media (30 mg/ml Todd-Hewitt Broth powder and 0.2 mg/ml yeast) ...
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bioRxiv - Microbiology 2019Quote: ... coli using the QIAprep® Spin Miniprep Kit (Qiagen) and the DNA was eluted with nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified on Ni-NTA agarose beads (Qiagen) using standard protocols ...
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bioRxiv - Immunology 2023Quote: ... coli cultures with AllPrep PowerFecal DNA/RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Genetics 2022Quote: ... primary erythroblasts or BFU-E using the RNeasy micro kit (QIAGEN). Reverse transcription of mRNA was performed using the SuperScriptIII First-Strand Synthesis System for RT-PCR (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Organs were homogenized by high-speed shaking in 2 mL microcentrifuge tubes filled with sterile PBS and 5/7 mm stainless steel beads using TissueLyser LT (Qiagen, Hilden, Germany).
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA from honey bees was isolated from the abdomen of individual bees after homogenization in 200 μl of Trizol reagent using the TissueLyser LT (Qiagen N. V.) for 3 min ...
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bioRxiv - Microbiology 2020Quote: ... Lung tissue was homogenized with a Qiagen TissueLyser LT and RNA extracted from supernatant using Qiagen RNeasy Mini Kit (Qiagen, Cat #74104) following manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Microbiology 2023Quote: ... The MLB and MLBE tubes were then subjected to bead-beating for 6 min using the TissueLyser Lt (Qiagen®, Hilden, Germany), with the instrument set at 50 Hz ...
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bioRxiv - Plant Biology 2023Quote: ... Collected tissue was ground to a fine powder at -80°C using 3 mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA), and between 10-15 mg of ground tissue per sample was used for auxin extraction ...
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bioRxiv - Genetics 2024Quote: ... Frozen tissues were disrupted in 50:50 cold methanol/0.1 M hydrogen chloride and homogenized at high speed by shaking with stainless-steel beads into a TissueLyser LT (Qiagen, Duesseldorf, Germany). The metabolites-containing supernatant was isolated from the protein pellet by centrifugation at 13,000 rpm for 60 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Electroblotted membranes were incubated with penta-His antibody (Qiagen, Valencia, CA, USA). The antigen–antibody complex was visualized with alkaline phosphatase-linked to anti-rabbit IgG followed by staining with 5-bromo-4-chloroindol-2-yl phosphate and nitro blue tetrazolium [36].
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Biochemistry 2021Quote: ... The primary and secondary antibodies used were anti-penta His (Qiagen #34660) and goat anti-mouse IgG (LiCOR #926-68070 ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tag ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse penta-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Microbiology 2019Quote: ... His-cyAbrB1 was purified with the Ni-NTA Fast Start kit (Qiagen). The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... His-NaERF2-like were expressed and purified with Ni-NTA agarose (QIAGEN). Biotin labeled probe EM13 (5’-tagattATCTaattctact-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-TRIM39 proteins were purified on Ni-NTA agarose beads (Qiagen, #1018244) and then eluted in a buffer containing 0.5 M imidazole before dialysis in PBS.
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bioRxiv - Cell Biology 2022Quote: ... and 6×His-Flag-Ub was conducted using Ni-NTA agarose (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... His-tag proteins were purified using a Ni-NTA purification system (Qiagen) by following the specification of the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... a conjugate of mouse monoclonal penta-His antibody and horseradish peroxidase (Qiagen) was used ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transiently transfected with HA-tagged RILP or ORP1L constructs using Effectine (Qiagen), harvested at 24 hours post-transfection and fixed in 3.7% formaldehyde in PBS for 15-20 min ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged Lem27 or its truncation mutants was purified using Ni2+-NTA columns (Qiagen). After washing with a buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... coli lysates using a nickel-nitrilotriacetic acid superflow resin (Qiagen) then combined and sent for anti-sera production (Covance) ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21-RIL and purified using Ni-NTA agarose (Qiagen) and Mono S affinity chromatography ...
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bioRxiv - Microbiology 2019Quote: ... coli cells and purified using the QiaQuick Miniprep kit (Qiagen) and quantified via Qubit Fluorometric Quantitation (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... coli DH5α cells and purified with Qiagen miniprep kit (Qiagen). The recovered plasmid stocks were verified through sequencing (Eton Bioscience).
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bioRxiv - Microbiology 2021Quote: ... coli pKAS32 cultures using a QIAprep Spin Miniprep Kit (Qiagen) and restriction digested with SacI and XbaI ...
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bioRxiv - Developmental Biology 2022Quote: ... Coli using the Plasmid Plus Mega Kit (Qiagen, Cat#12981). To assess library complexity ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21 RIL cells and purified on NiNTA beads (Qiagen), then concentrated and desalted using Vivaspin 500 columns (Sartorius).
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bioRxiv - Biochemistry 2019Quote: ... coli DH5α cells following QIAprep Spin Miniprep Kit protocol (QIAGEN) and used to transform the expression strains E ...
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bioRxiv - Molecular Biology 2020Quote: ... coli with the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions and subsequently sequenced using the BigDye Terminator v1.1 Cycle Sequencing Kit and an ABI 3130 capillary sequencer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... coli cell lysate using Ni-nitriloacetic acid agarose resin (Qiagen) under denaturing conditions according to the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using an endotoxin-free Maxi kit (Qiagen, ON, Canada) and resuspended in nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... coli TOP10 using a QIAprep Spin Miniprep Kit (Qiagen, Germany) and was verified by sequencing (using 9 pairs of overlapping primers ...