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Citations for Qiagen :
401 - 450 of 1566 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: All plasmid vectors were prepared by Qiagen midi prep and 10-20 ug of DNA linearized by digest with KpnI-HF and XbaI restriction enzymes at 37°C in NEB Cutsmart buffer with enough units of activity to generate ~5X overdigest in two hours (New England Biolabs ...
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bioRxiv - Neuroscience 2019Quote: ... and QIAGEN Plasmid Maxi Kit (Qiagen, 12162).
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bioRxiv - Biochemistry 2021Quote: ... and plasmids were isolated by Miniprep (Qiagen). This plasmid pool was used to generate a lentiviral library ...
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bioRxiv - Cell Biology 2021Quote: ... and Qiagen Plasmid Midi kit (Qiagen 12143). All constructs were verified by sequencing (Eton Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... using a QIAfilter Plasmid Midi Prep (Qiagen). Wild type strain 854 or 854ΔhsdM was subjected to electroporation with 1 μg of plasmid DNA ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids for transfection were prepared by Qiagen maxiprep.
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were transfected using Effectene (Qiagen, Germany) according to the manufacturer’s protocols for 22 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... purified using the Plasmid Maxi Kit (Qiagen), and used as a DNA template vector for cleavage experiments ...
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bioRxiv - Genetics 2022Quote: ... by the plasmid maxi-kit (Qiagen Inc.)
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bioRxiv - Immunology 2022Quote: ... Recombinant plasmids were then isolated (MiniPrep, Qiagen) and sequenced (DNA Sequencing Facility ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli DH5α using Plasmid Miniprep Kit (Qiagen) for transformation of B ...
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bioRxiv - Genomics 2023Quote: ... purified using Plasmid plus midi kit (QIAGEN) and tested for its quality via sequencing on a MiSeq (see below section).
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bioRxiv - Biophysics 2023Quote: ... using Qiafilter plasmid midi kit (Qiagen, Germany). These plasmids were digested with NotI-HF and XhoI for 2 h at 37°C (R3189 ...
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bioRxiv - Microbiology 2023Quote: ... and extracted using Plasmid Maxi Kit (QIAGEN), according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Plasmids were isolated (Qiagen Miniprep kit, 27106) and sequenced for appropriate insertion ...
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bioRxiv - Developmental Biology 2023Quote: ... plasmids were extracted using Miniprep kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were extracted by mini-prep (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... and plasmids were recovered by miniprep (Qiagen). Plasmids were checked for proper gene insertion by Sanger sequencing (Genewiz).
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bioRxiv - Immunology 2023Quote: ... and the plasmids were purified by QIAGEN plasmid plus midi kit according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... and plasmids were recovered by miniprep (Qiagen). Plasmids were checked for proper gene insertion by Sanger sequencing (Genewiz).
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bioRxiv - Developmental Biology 2023Quote: ... amplified using Plasmid Maxi Kits (Qiagen 12163) and verified by Sanger sequencing before egg injection ...
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bioRxiv - Microbiology 2019Quote: ... Singe transformants were then grown in batch culture for plasmid extraction using the Plasmid Midi Kit (Qiagen, Germantown, MD). KPPR1ΔgltA competent cells were prepared as previously described [26] ...
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bioRxiv - Molecular Biology 2021Quote: HOS cells were cotransfected with a Rev/Env-encoding pSVIIIenv plasmid and a Tat-encoding plasmid at a 1:0.125 ratio using the Effectene transfection reagent (Qiagen). At 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Single transformants were then grown in batch culture for plasmid extraction using the Plasmid Midi Kit (Qiagen, Germantown, MD). MG1655 and NTUH-K2044 competent cells were prepared as previously described45 ...
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bioRxiv - Immunology 2022Quote: ... The recombinant plasmids for mouse vaccination were prepared using an EndoFree Plasmid Purification Kit (Cat# 12391, Qiagen, Hilden, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Milligram quantities of the various plasmids used in this work were purified using the Plasmid Giga Kit (QIAGEN, Germany) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... and pooled plasmids for each half library were isolated separately by Maxipreps using an EndoFree Plasmid Maxi Kit (Qiagen). To prepare the pooled lentiviral library ...
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bioRxiv - Genomics 2020Quote: ... 50ml of overnight E.coli culture was harvested to purify the Aβ plasmid library with a midi prep (Plasmid Midi Kit, Qiagen). The resulting library contained 29.9% of WT Aβ ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were collected by centrifuging the plates for 10 min at 1,200 x g and the plasmids’ DNA was purified using the QIAGEN Plasmid Plus 96 Miniprep Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2023Quote: ... all the colonies were collected as a pool for plasmid library extraction with Endotoxin-Free Plasmid Maxiprep (Qiagen, #12362).
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNAs of positive clones were propagated and purified using the standard Qiagen maxiprep plasmid purification kit (#12163, Qiagen). HEK-293 cells (ATCC type ...
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bioRxiv - Biophysics 2023Quote: ... coli culture were harvested next morning to extract the mutagenesis plasmid library using the QIAfilter Plasmid Midi Kit (QIAGEN). To obtain the final libraries into the yeast plasmids ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen). The isolated plasmid was subsequently transformed into D.radiodurans R1 chemically competent cells (above) ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNAs were isolated from cerebral cortices (P15 rats) using Mini RNeasy kit (Qiagen) then converted to cDNA using 1 µg RNA and a QuantiTect Reverse Transcription kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... A customized RT2 Profiler PCR array for rat angiogenesis markers (PARN 042Z, 330231, Qiagen) containing SYBR Green RT PCR assays for 84 genes of interest ...
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bioRxiv - Cancer Biology 2021Quote: ... Inoculants from glycerol stocks or stab culture were cultured overnight in liquid LB at 37°C and plasmids were extracted using Plasmid miniprep kit (Qiagen). See Key Resources Table for shRNA identity ...
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bioRxiv - Genomics 2019Quote: ... was used to incorporate nicks in one of the plasmids (extracted from bacteria with the Plasmid Plus Midi Kit, Qiagen) used ...
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bioRxiv - Immunology 2019Quote: ... Cells were expanded in an overnight liquid culture and the pooled plasmid library was extracted using QIAfilter Plasmid Maxi Kit (Qiagen). If necessary ...
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bioRxiv - Genetics 2020Quote: ... were cloned into pX458 plasmid backbone68 by the Genome Engineering Facility (WIMM, University of Oxford) and purified using Plasmid Midi Kit (Qiagen). pX458 (pSpCas9(BB)-2A-GFP ...
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bioRxiv - Cancer Biology 2020Quote: ... coli TOP10 cells were used again for preparation and amplification of the resulting destination plasmid that was subsequently purified by the Qiagen Plasmid Maxi Kit (Qiagen). The resulting destination plasmid was stored at -20 °C.
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bioRxiv - Synthetic Biology 2021Quote: ... from our assembly reaction transformation into LB and immediately purified their plasmid DNA using the Plasmid Plus Midi Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... We incubated these cultures on a shaker at 225 rpm and 32°C for 12-14 hours then pelleted the cultures by centrifugation and isolated plasmid using the EndoFree plasmid maxiprep kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: 293FT cells were transfected with pMSCV-dnCRTC or pMSCV-GFP constructs together with packaging plasmid pMD.MLV and pseudotyped envelope plasmid pMD2-VSV-G using Effectene transfection reagent (Qiagen #301425) as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen) the next day ...
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bioRxiv - Immunology 2022Quote: ... The next day cells were washed off the plates and plasmids were purified using the HiSpeed Plasmid Maxi kit (Qiagen). The resulting replicate libraries were packaged into a lentiviral library using the packaging vectors psPAX2 and pMD2G and the LV-MAX lentiviral production system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with 500 ng of pGL3- DARPP-32 plasmids and 50 ng of pRL-SV40 plasmids (renilla; internal reference control) using Polyfect transfection reagent (Qiagen). Lastly ...
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bioRxiv - Genetics 2020Quote: ... the bacterial colonies were scraped into a collection bottle and plasmid pools were extracted with a HiSpeed Plasmid Maxiprep kit (QIAgen). 2 - 3 small plates were prepared in parallel with diluted transformed cells in order to count colonies and confirm the transformation efficiency was sufficient to maintain at least 30x library coverage ...
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bioRxiv - Immunology 2021Quote: ... These plasmids were purified and DNA extracted using QIAGEN Plasmid Midi Kit (100) (cat. n° 12145, QIAGEN, Valencia, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... Cell pellets were collected by centrifugation at 6000xg for 15min and plasmid DNA was isolated using a QIAfilter Plasmid Giga Kit (Qiagen).
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bioRxiv - Genetics 2019Quote: ... Bacterial cells were cultured overnight and plasmid DNA encoding an intermediate library was extracted using QIAGEN Plasmid Midi Kit (QIAGEN). A dsDNA gBlock (IDT ...