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Citations for Qiagen :
601 - 650 of 1566 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Plasmids were purified by using Spin Miniprep Kit (Qiagen). Sequencing (Eurofins Genomics ...
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bioRxiv - Biochemistry 2023Quote: ... MN (Germany) or Plasmid Midi kits from Qiagen (Germany).
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bioRxiv - Developmental Biology 2024Quote: ... Expression plasmids were transfected using Effectene transfection reagent (QIAGEN). Plasmids were generated via Gateway® technology (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Pathway-specific primer mixes (Rat Antibacterial Response, PBR-148Z, and Human Antibacterial Response, PBH-148Z; Qiagen) were used for preamplification and qPCR arrays (Rat Antibacterial Response ...
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bioRxiv - Neuroscience 2020Quote: Total hippocampus RNA was isolated from 4-month olds rats using RNeasy Lipid Tissue Kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... All constructs were sequenced before use and plasmids used for transfection experiments were purified with the EndoFree® plasmid maxi kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... The ligated cDNA was transformed into XL10-gold and the plasmids were purified using a plasmid plus midi kit (Qiagen, Germantown, MD, USA). The complexity of the cDNA library was 2 × 106 ...
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bioRxiv - Microbiology 2023Quote: ... bacterial colonies were pooled into LB-ampicillin culture media and amplified followed by plasmid purification with the Plasmid Maxi Kit (Qiagen, Cat. No. 12165). Barcode diversity in the plasmid stock was confirmed via next-generation sequencing (Amplicon-EZ – Azenta Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: We isolated genomic DNA from rat tail tips using Qiagen genomic DNA extraction kit (Qiagen Inc., USA). We performed genotyping via PCR ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from rat or mouse hippocampal cultures using the RNeasy Mini Kit (Qiagen, #74104). DNA contamination was removed by digestion with DNase I (Qiagen ...
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bioRxiv - Immunology 2021Quote: Total DNA was extracted from Wistar rat tissues samples using the DNeasy Blood & Tissue Kit (Qiagen, Germany) as per manufacturer’s instructions ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... B220 (RA3-6B2) and Ly-6C/G (RB6-8C5) and anti-rat IgG-coupled magnetic beads (Qiagen) as previously described [34] ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from rat lower esophageal tissues using the RNeasy Fibrous Tissue Kit (Qiagen, Germantown, MD). Each sample was homogenized in 400 µL of Buffer RLT with beta-mercaptoethanol for 3 – 10 second pulses with the homogenizer (Pro-Scientific Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... genomic DNA was extracted from rat PSC cell pellets using the DNeasy Blood and Tissue kit (Qiagen) and PCR was performed using the Platinum PCR SuperMix High-Fidelity kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified using QIAprep Spin Miniprep kit (Qiagen) for Sanger sequencing (Macrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was isolated (QIAprep Spin Miniprep Kit, Qiagen, 27104) from each transformant and subsequently 5 μL plasmid DNA (80-100 ng/μL ...
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bioRxiv - Biophysics 2022Quote: ... After purification using a Qiagen Maxiprep plasmid purification kit (Qiagen). The 64x repeat was then cleaved out of the plasmid using XbaI ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were extracted and purified using a Miniprep Kit (QIAGEN), and sequenced (GENEWIZ) ...
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bioRxiv - Developmental Biology 2019Quote: ... using 2µg of plasmid DNA and Polyfect transfection reagent (Qiagen). Positive selection was performed by adding 25µg/ml of hygromycin B (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were prepared for injection using standard miniprep extraction (Qiagen) and submitted for injection (Microinjection service ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmid DNA was purified using QuickLyse Miniprep Kit (Qiagen, USA). The construct containing OGR1 was further confirmed by sequencing using the ABI Prism 377 Automated DNA Sequencer (Applied Biosystems ...
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bioRxiv - Physiology 2019Quote: ... plasmids were extracted using the QIAprep Spin Miniprep kit (Qiagen) and sequenced by ABI 3730 automated DNA sequencer at Davis Sequencing (Davis ...
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bioRxiv - Cell Biology 2019Quote: Plasmids were transfected for 22 hours using Effectene (Qiagen, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Genomics 2019Quote: ... We miniprepped plasmids using the Qiaprep Spin Mini kit (Qiagen) and verified plasmid sequences through Sanger sequencing with the RV3 primer.
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bioRxiv - Genetics 2019Quote: ... DNA maxiprep kit was from Qiagen (EndoFree Plasmid Maxi Kit). All plasmids were cloned with Gibson assembly (42 ...
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bioRxiv - Cancer Biology 2020Quote: ... The phagemid DNA was isolated using plasmid miniprep kit (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... dsDNA was extracted using the Plasmid Miniprep Kit (Qiagen, 27104). dsDNA concentration was measured using a Nanodrop (Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids were purified using the QIAprep spin miniprep kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... Plasmid DNAs were initially isolated using a maxiprep kit (Qiagen). To remove nicked plasmid species ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected in U87 cells with Effectene (Qiagen, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2021Quote: ... isolated with the use of a Plasmid Maxi Kit (Qiagen), and then subjected to in vitro transcription in a transcription mixture of 100 μl containing 10 μg of plasmid as template DNA ...
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bioRxiv - Biochemistry 2020Quote: ... The vector was isolated using Qiagen plasmid maxi kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid and DNA fragments were purified using kits from Qiagen according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... or the pEhEx plasmid backbone using Attractene transfection reagent (Qiagen). Stable transfectants were initially selected at 3 µg/ml Geneticin (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were prepared using the QIAprep Spin Miniprep Kit (Qiagen). Sanger sequencing was performed by Genewiz using M13 forward and reverse primers ...
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bioRxiv - Microbiology 2019Quote: ... The plasmids were transfected into RAW264.7 with Attractene Reagent (Qiagen) and the overexpression efficiency determination or the following assays were also carried out 48 h later.
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bioRxiv - Microbiology 2019Quote: ... Plasmids were extracted and purified using a Miniprep Kit (Qiagen) and sequenced by Sanger Sequencing at the Plate-forme d’Analyse Génomique of Laval University ...
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bioRxiv - Microbiology 2019Quote: ... and purified plasmid with the QIAprep Spin Miniprep Kit (Qiagen). Each plasmid was Sanger sequenced using the pRCS1seqR primer (S4 Table).
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Plasmids were prepared using the QIAprep Spin Miniprep Kit (Qiagen). Sanger sequencing was performed by Genewiz using M13 forward and reverse primers ...
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bioRxiv - Genomics 2020Quote: ... The plasmid was then purified via maxiprep (Qiagen Hilden, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were extracted by a QIAprep spin miniprep kit (Qiagen) and sequenced ...
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bioRxiv - Biochemistry 2022Quote: ... and purified with the Qiagen Plasmid Plus Midiprep kit (Qiagen) for transfections.
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bioRxiv - Genomics 2022Quote: ... Plasmids were purified by mini prep (QIAprep Miniprep Kit, Qiagen) and transformed into yeast cells using one transformation tube of the transformation protocol described above ...
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bioRxiv - Genomics 2022Quote: ... the cultured bacteria was pooled prior plasmid purification (Qiagen, 12963). 20 μg of Δorf duo library plasmid was digested with180 U of PmeI and 20 U of AsiSI at 37°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Coli using the Plasmid Plus Mega Kit (Qiagen, Cat#12981). To assess library complexity ...
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bioRxiv - Microbiology 2020Quote: ... 100 μg of plasmid DNA (maxi prepped using Qiagen kit) was preloaded into O+ RBCs at 50% hematocrit in cytomix prior to culturing with parasite-infected RBCs ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid isolations were performed using QIAprep spin miniprep kits (Qiagen).