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Citations for Qiagen :
251 - 300 of 5585 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were subsequently passed through a QIAshredder column (Qiagen, 79654) to eliminate debris before proceeding with nucleic acid purification ...
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bioRxiv - Bioengineering 2024Quote: ... Cleared lysate was applied to a Ni-NTA column (Qiagen) and eluted using DPBS containing 250 mM imidazole.
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was further treated with 25 µL of proteinase K (20 mg/mL) (Qiagen Singapore Pte. Ltd, Cat. No. 19133), gently mixed by flicking of tube and incubated at 56 °C for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
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bioRxiv - Immunology 2023Quote: ... followed by magnetic depletion using goat anti-rat beads (QIAGEN). For adoptive transfer experiments ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified and bound to a Ni-NTA column (Qiagen) for 1h ...
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bioRxiv - Genomics 2019Quote: ... Lysates were washed twice with 650 µL of PE buffer (Qiagen) and spun through the columns at 6000 rpm for 1 minute ...
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bioRxiv - Biochemistry 2019Quote: ... the cleared lysate was loaded onto a Ni-NTA column (Qiagen) and eluted with an imidazole gradient ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was purified from the lysate using RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Centrifugation-cleared lysate was applied to Ni-NTA agarose beads (Qiagen), washed with 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Centrifugation-cleared lysate was applied to Ni-NTA agarose beads (Qiagen), washed with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Centrifugation-cleared lysate was applied to Ni-NTA agarose beads (Qiagen), washed with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... Fusion proteins were purified from clarified lysate by Ni-NTA (Qiagen) affinity chromatography ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... Clarified lysate was incubated with Ni-NTA agarose (Qiagen, Cat# 30210) for 1 h at 4 °C and after washing with buffer A + 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was incubated with Strep-Tactin Superflow resin (Qiagen) for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The clear lysate was incubated with Ni-NTA agarose beads (Qiagen) for 2 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Lysates were incubated with 1 ml of Ni-NTA resin (QIAGEN) in 4°C for 1 hr ...
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bioRxiv - Microbiology 2019Quote: ... The homogenized lysate was passed through genomic DNA eliminator columns (Qiagen) and subsequently applied to RNeasy spin columns ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was loaded onto preequilibrated Ni-NTA beads (30230, Qiagen) by gravity-flow ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was loaded onto preequilibrated Ni-NTA beads (30230, Qiagen) by gravity-flow ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was loaded onto preequilibrated Ni-NTA beads (30230, Qiagen) by gravity-flow ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared lysate was loaded onto a Ni-NTA column (Qiagen) and eluted with an imidazole gradient ...
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bioRxiv - Neuroscience 2019Quote: ... The cleared lysate was loaded onto a gravity NiNTA column (Qiagen), washed with 8M urea ...
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bioRxiv - Genetics 2020Quote: ... Worm lysates were generated by TissueLyser with steel beads (Qiagen #69989), and re-suspended in 500 μl of the lysis buffer containing 0.8 M sucrose ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) for 1 hr and washed with 50 mL wash buffer (1X PNI ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted from lysates using an RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was extracted from lysates using an RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... After the lysate was homogenized using the QIAshredder Kit (Qiagen; 79656), mRNA was extracted using the RNeasy Plus Micro Kit and eluted in 30μL of RNAse-free water ...
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bioRxiv - Microbiology 2022Quote: ... and lysate was passed over 8 mL Ni-NTA resin (Qiagen) using gravity chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... the lysate was processed using total RNeasy mini kit (Qiagen, Germany). The lysate was resuspended in a 1:1 volume of 70% ethanol and loaded onto an extraction column and centrifuged (8000g for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate-ethanol mix was then purified using RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and clarified lysate was incubated with Ni-NTA affinity resin (Qiagen) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
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bioRxiv - Cell Biology 2023Quote: ... and clarified lysate was loaded into Ni-NTA Agarose (Qiagen, USA) gravity column pre-equilibrated with lysis buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cleared lysates were incubated with 2-4ml nickel NTA beads (Qiagen) for 20-40 minutes before washing beads with 5-10 column volumes of lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with Ni-NTA agarose beads (Qiagen) for 1.5 hours ...
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bioRxiv - Physiology 2023Quote: ... The tissue lysates were treated with 1% DNase (Qiagen, Hilden, Germany) and diluted to a protein concentration of 10μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was loaded onto tandem Streptactin XP columns (QIAGEN), equilibrated in Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate was applied to the Ni-NTA agarose resin (Qiagen) and the resin was washed three times using a buffer [20 mM Tris-HCl (pH 8) ...
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bioRxiv - Developmental Biology 2023Quote: ... Lysates were thawed on ice and the remaining steps from Qiagen kit were carried out at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were then homogenized by passing them through QIAshredder cartridges (Qiagen). Proteins were precipitated by adding an equal volume of ice cold 55% Trichloroacetic acid (TCA ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified bacterial lysate was then incubated with Ni-NTA agarose (Qiagen) at 4 °C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was then purified with Ni-NTA resin (Qiagen) in batch mode ...
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bioRxiv - Physiology 2023Quote: ... Tissue lysates were further clarified by microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate centrifuged at 10,000g for 2min at 41C ...
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bioRxiv - Microbiology 2024Quote: ... Cleared lysate was bound to Strep-Tactin Superflow Plus resin (Qiagen). The resin was washed first with lysis buffer and then with RNP buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified lysate was incubated with 0.5 mL NiNTA resin (QIAGEN) for 1 hr at 4°C on a rotary ...
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bioRxiv - Microbiology 2024Quote: ... cleared lysates were incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were mixed with anti-rat IgG-conjugated BioMag beads (QIAgen) and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec) ...