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Citations for Qiagen :
51 - 100 of 5585 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Total RNA was prepared from cell lysates using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Set (Qiagen ...
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bioRxiv - Immunology 2019Quote: Total RNA was isolated from cell lysates using the RNeasy Micro Kit (Qiagen) including on-column DNase digestion ...
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bioRxiv - Microbiology 2020Quote: ... and RNA from cell lysates was extracted using a RNeasy Mini Kit (Qiagen) as described previously (20 ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from infected cell lysates using RNeasy® Mini Kit (QIAGEN) with an on-column DNase treatment (QIAGEN ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from whole-cell lysates with RNeasy Plus Mini kit (Qiagen) and was reverse-transcribed with cDNA synthesis kit (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from cell lysates using the RNeasy Micro Kit (QIAGEN), including on-column deoxyribonuclease digestion ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was prepared from cell lysates using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Set (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysates were either mock-treated or treated with RNase A (Qiagen 19101) and then incubated at 4°C for 2 hours with biotinylated oligos ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered cell lysate was applied to 10 mL of Ni-NTA resin (Qiagen) which had been previously equilibrated with 10 column volumes of H2O ...
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bioRxiv - Bioengineering 2023Quote: ... RNA extraction was performed from each cell lysate employing a miniprep kit (Qiagen). The RNA quality was analyzed using the 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... genomic DNA was isolated from cell lysates with a QIAamp DNA Mini Kit (Qiagen). Barcode sequences were amplified by PCR using 300 ng of genomic DNA (100,000-genome and 500,000-barcode equivalent ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen), and protein was purified following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant of the cell lysate was loaded onto Ni-NTA agarose resin (Qiagen) in Poly-prep chromatography columns (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was then extracted from cell lysates using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNAs were extracted from homogenized cell lysate using RNEasy Plus mini kit (Qiagen), and the quality of extracted total RNA were examined using Nanodrop spectrum analysis and agarose gel electrophoresis ...
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bioRxiv - Genetics 2020Quote: ... Cell lysates were incubated with 100 μl of Ni-NTA superflow beads (Qiagen, 30430) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted from total cell lysate and cytosolic fractions using DNeasy kit (Qiagen). DNA samples were each diluted 1:10 and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Bioengineering 2022Quote: ... cell lysates were harvested using the RNeasy Mini kit (Qiagen #74104, Germantown, MD, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were processed for total RNA extraction using the RNeasy Micro Kit (Qiagen) and treatment with RNase-free DNase using the Ambion™ DNase I protocol (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from cell lysates and purified with RNeasy Mini Kit (Qiagen) or Direct-zolTM RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Systems Biology 2023Quote: ... total RNA was extracted from cell lysate by RNeasy Plus Mini Kit (Qiagen, 74136). Total RNA which was carried out following the method described above was removed rRNA by rRNA Depletion Kit (Vazyme ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was extracted from thawed cell lysates using the RNeasy Plus Micro Kit (QIAGEN) following the manufacturer’s instructions and genomic DNA depleted using the supplied gDNA eliminator columns ...
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bioRxiv - Genomics 2024Quote: ... total RNA was isolated from cell lysates using AllPrep DNA/RNA Mini Kit (QIAGEN). RNA quality was assessed based on RNA integrity number (RIN ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were treated with 4 μl of Qiagen protease (QIAGEN, Hilden, Germany, #1017782,) at 56°C for 10 min ...
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bioRxiv - Genomics 2024Quote: RNA from cell lysates was extracted using the Qiagen RNeasy mini kit (Qiagen 74106). Cell lysates were extracted in a randomized order to prevent batch effects in downstream analysis ...
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bioRxiv - Genomics 2020Quote: ... The supernatant of cell lysate was incubated with 1 mL Ni-NTA agarose beads (QIAGEN) at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Valencia, CA, USA), according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... The soluble cell lysate fraction was loaded on a 4 mL Ni-NTA column (QIAGEN) pre-equilibrated with binding buffer ...
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bioRxiv - Biophysics 2019Quote: ... All the proteins were obtained in cell lysates and subsequently purified using Ni-NTA (Qiagen) affinity-columns and superdex 200 increase 10/300 GL (GE Lifesciences ...
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bioRxiv - Genomics 2019Quote: ... The cell lysate was sampled (100 μL) and mixed with 700 μL of QIAzol (QIAGEN) for total RNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... The clarified cell lysate was loaded onto a 5 mL Ni-NTA superflow cartridge (Qiagen), washed with buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was centrifuged and the soluble cell fraction was incubated with Ni2+ –NTA (Qiagen) beads for 3 hr ...
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bioRxiv - Microbiology 2020Quote: ... and then cell lysates were collected by washing cells twice with PBS and adding 100ul of RNA lysis buffer (Qiagen) to each well.
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2μg was used for cDNA synthesis (Omniscript RT kit) ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from rat blood cells using QIAmp DNA Mini Kit (Qiagen, City, state) following manufacturer’s instructions using a QIAcube automated device ...
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bioRxiv - Genetics 2020Quote: DNA/RNA extraction was performed from cell lysates using the AllPrep DNA/RNA Micro Kit (Qiagen). For initial optimisation experiments using an HPRT gRNA-containing RNP (or non-targeting control) ...
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bioRxiv - Genetics 2020Quote: ... Total DNA was isolated from the cell lysates using the QIAquick® PCR Purification Kit (QIAGEN). The following primers used were designed to the lucia DNA sequence for amplification of the reporter gene (JE041 ...
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bioRxiv - Microbiology 2021Quote: ... Toxins were then purified from bacterial cell lysates through affinity chromatography on a Nickel-resin (Qiagen), ion exchange ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acids were isolated from cell lysates or lung homogenates using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were spun on the QIashredder column and RNA extracted using the RNeasy kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was isolated from Trizol/cell lysate mixture by using RNAeasy Mini Kit (Qiagen #74104). Total RNA was sequenced on Illumina NextSeq using Illumina TruSeq Stranded Total RNA library Prep and paired-end sequencing ...
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bioRxiv - Microbiology 2023Quote: ... total RNA of infectious cell lysate was extracted using an RNeasy Mini Kit (QIAGEN, Hilden, Germany). RT–PCR was performed using a Superscript III One-Step RT-PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA (tRNA) from the cell lysate were extracted immediately using Qiagen RNeasy Microkit (Qiagen, Germany) before reverse transcription into cDNA using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and clarified lysate was loaded onto a Strep-Tactin column (QIAGEN). The column was washed with 20 CVs of wash buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysate was treated with DNaseI (Qiagen) for 15 min at room temperature (RT) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was clarified by centrifugation and soluble lysate was incubated with Ni-NTA Agarose beads (Qiagen, 30210) for 2 hr at 4 °C.